A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a
Ciprofloxacin (CIP) is a broad-spectrum fluoroquinolone antibiotic, and its excessive residues in food and water sources pose potential risks to human health. Therefore, there is a need for a rapid and convenient method for its accurate quantification. The clustered regularly interspaced short palin...
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Elsevier
2025-02-01
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| Series: | Molecular and Cellular Probes |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0890850824000501 |
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| author | Fangyue Guo Jianghao Li Peizhi Ma Mengying Liu Jing Wu Hai Qu Yehuan Zheng Mengying Wang Seyed Sepehr Marashi Zhijian Zhang Shanfeng Zhang Guangyu Fu Pei Li |
| author_facet | Fangyue Guo Jianghao Li Peizhi Ma Mengying Liu Jing Wu Hai Qu Yehuan Zheng Mengying Wang Seyed Sepehr Marashi Zhijian Zhang Shanfeng Zhang Guangyu Fu Pei Li |
| author_sort | Fangyue Guo |
| collection | DOAJ |
| description | Ciprofloxacin (CIP) is a broad-spectrum fluoroquinolone antibiotic, and its excessive residues in food and water sources pose potential risks to human health. Therefore, there is a need for a rapid and convenient method for its accurate quantification. The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas12a system has gained extensive application in signal detection and amplification due to the trans-cleavage activity of Cas12a. In this study, we devised a novel magnetic bead-based dual sandwich aptamer coupled with a CRISPR/Cas12a system for the precise quantification of CIP in milk, river water, and honey. Through the incorporation of a magnetic bead-based dual aptamer sandwich approach, the concentration of CIP in the samples was pre-enriched. Additionally, by optimizing the Fluorescence-Quencher (F-Q) probe concentration, detection aptamer (APTd) concentration, and assay duration, the limit of blank (LOB) of the system was determined as 362 nM, while the limit of detection (LOD) was determined as 403 nM. This enabled the accurate quantification of CIP within the linear range of 0.5 μM to 0.2 mM with high specificity. Moreover, the performance of this detection method was comparable to that of high-performance liquid chromatography (HPLC) in river water, milk, and honey samples. |
| format | Article |
| id | doaj-art-fb5eb7825bd64cf79b9ae53f03c4db61 |
| institution | OA Journals |
| issn | 0890-8508 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular and Cellular Probes |
| spelling | doaj-art-fb5eb7825bd64cf79b9ae53f03c4db612025-08-20T02:13:52ZengElsevierMolecular and Cellular Probes0890-85082025-02-017910199810.1016/j.mcp.2024.101998A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12aFangyue Guo0Jianghao Li1Peizhi Ma2Mengying Liu3Jing Wu4Hai Qu5Yehuan Zheng6Mengying Wang7Seyed Sepehr Marashi8Zhijian Zhang9Shanfeng Zhang10Guangyu Fu11Pei Li12School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; School of Basic Medical Sciences Innovation and Entrepreneurship Base for College Students, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Department of the First Clinical Medicine, Zhengzhou University, Zhengzhou, Henan, 450052, PR ChinaR & D Center, Autobio Diagnostics Co., Ltd, Zhengzhou, Henan, 450016, PR ChinaSchool of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; School of Basic Medical Sciences Innovation and Entrepreneurship Base for College Students, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Department of the First Clinical Medicine, Zhengzhou University, Zhengzhou, Henan, 450052, PR ChinaSchool of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; School of Basic Medical Sciences Innovation and Entrepreneurship Base for College Students, Zhengzhou University, Zhengzhou, Henan, 450001, PR ChinaSchool of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; School of Basic Medical Sciences Innovation and Entrepreneurship Base for College Students, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Department of the First Clinical Medicine, Zhengzhou University, Zhengzhou, Henan, 450052, PR ChinaR & D Center, Autobio Diagnostics Co., Ltd, Zhengzhou, Henan, 450016, PR ChinaSchool of Basic Medical Sciences, Xi'an Jiaotong University, Xian, 710061, PR ChinaState Key Laboratory of Biotherapy, Sichuan University, Chengdu, 610041, PR ChinaCollege of International Education, Zhengzhou University, Zhengzhou, Henan, 450001, PR ChinaSchool of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR ChinaSchool of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; School of Basic Medical Sciences Innovation and Entrepreneurship Base for College Students, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Corresponding author. School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China.R & D Center, Autobio Diagnostics Co., Ltd, Zhengzhou, Henan, 450016, PR China; Corresponding author.School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; School of Basic Medical Sciences Innovation and Entrepreneurship Base for College Students, Zhengzhou University, Zhengzhou, Henan, 450001, PR China; Corresponding author. School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China.Ciprofloxacin (CIP) is a broad-spectrum fluoroquinolone antibiotic, and its excessive residues in food and water sources pose potential risks to human health. Therefore, there is a need for a rapid and convenient method for its accurate quantification. The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas12a system has gained extensive application in signal detection and amplification due to the trans-cleavage activity of Cas12a. In this study, we devised a novel magnetic bead-based dual sandwich aptamer coupled with a CRISPR/Cas12a system for the precise quantification of CIP in milk, river water, and honey. Through the incorporation of a magnetic bead-based dual aptamer sandwich approach, the concentration of CIP in the samples was pre-enriched. Additionally, by optimizing the Fluorescence-Quencher (F-Q) probe concentration, detection aptamer (APTd) concentration, and assay duration, the limit of blank (LOB) of the system was determined as 362 nM, while the limit of detection (LOD) was determined as 403 nM. This enabled the accurate quantification of CIP within the linear range of 0.5 μM to 0.2 mM with high specificity. Moreover, the performance of this detection method was comparable to that of high-performance liquid chromatography (HPLC) in river water, milk, and honey samples.http://www.sciencedirect.com/science/article/pii/S0890850824000501Dual-aptamer sandwich assayCiprofloxacinCRISPR/Cas12aDetection |
| spellingShingle | Fangyue Guo Jianghao Li Peizhi Ma Mengying Liu Jing Wu Hai Qu Yehuan Zheng Mengying Wang Seyed Sepehr Marashi Zhijian Zhang Shanfeng Zhang Guangyu Fu Pei Li A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a Molecular and Cellular Probes Dual-aptamer sandwich assay Ciprofloxacin CRISPR/Cas12a Detection |
| title | A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a |
| title_full | A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a |
| title_fullStr | A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a |
| title_full_unstemmed | A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a |
| title_short | A magnetic bead-based dual-aptamer sandwich assay for quantitative detection of ciprofloxacin using CRISPR/Cas12a |
| title_sort | magnetic bead based dual aptamer sandwich assay for quantitative detection of ciprofloxacin using crispr cas12a |
| topic | Dual-aptamer sandwich assay Ciprofloxacin CRISPR/Cas12a Detection |
| url | http://www.sciencedirect.com/science/article/pii/S0890850824000501 |
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