Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China
Clostridioides difficile sequence type (ST) 37 (ribotype 017) is one of the most prevalent genotypes circulating in China. However, its genomic evolution and virulence determinants were rarely explored. Whole-genome sequencing, phylogeographic and phylogenetic analyses were conducted for C. difficil...
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1943538 |
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| author | Xingxing Xu Yuo Luo Huan Chen Xiaojun Song Qiao Bian Xianjun Wang Qian Liang Jianhong Zhao Chunhui Li Guangzhong Song Jun Yang Lingli Sun Jianmin Jiang Huanying Wang Bo Zhu Guangyong Ye Liang Chen Yi-Wei Tang Dazhi Jin |
| author_facet | Xingxing Xu Yuo Luo Huan Chen Xiaojun Song Qiao Bian Xianjun Wang Qian Liang Jianhong Zhao Chunhui Li Guangzhong Song Jun Yang Lingli Sun Jianmin Jiang Huanying Wang Bo Zhu Guangyong Ye Liang Chen Yi-Wei Tang Dazhi Jin |
| author_sort | Xingxing Xu |
| collection | DOAJ |
| description | Clostridioides difficile sequence type (ST) 37 (ribotype 017) is one of the most prevalent genotypes circulating in China. However, its genomic evolution and virulence determinants were rarely explored. Whole-genome sequencing, phylogeographic and phylogenetic analyses were conducted for C. difficile ST37 isolates. The 325 ST37 genomes from six continents, including North America (n = 66), South America (n = 4), Oceania (n = 7), Africa (n = 9), Europe (n = 138) and Asia (n = 101), were clustered into six major lineages, with region-dependent distributions, harbouring an array of antibiotic-resistance genes. The ST37 strains from China were divided into four distinct sublineages, showing five importation times and international sources. Isolates associated with severe infections exhibited significantly higher toxin productions, tcdB mRNA levels, and sporulation capacities (P < 0.001). Kyoto Encyclopedia of Genes and Genomes analysis showed 10 metabolic pathways were significantly enriched in the mutations among isolates associated with severe CDI (P < 0.05). Gene mutations in glycometabolism, amino acid metabolism and biosynthesis virtually causing instability in protein activity were correlated positively to the transcription of tcdR and negatively to the expression of toxin repressor genes, ccpA and codY. In summary, our study firstly presented genomic insights into genetic characteristics and virulence association of C. difficile ST37 in China. Gene mutations in certain important metabolic pathways are associated with severe symptoms and correlated with higher virulence in C. difficile ST37 isolates. |
| format | Article |
| id | doaj-art-fb5a3e1bb6474f9f839d522b6df72ef8 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-fb5a3e1bb6474f9f839d522b6df72ef82025-08-20T02:30:45ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-011011331134510.1080/22221751.2021.1943538Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in ChinaXingxing Xu0Yuo Luo1Huan Chen2Xiaojun Song3Qiao Bian4Xianjun Wang5Qian Liang6Jianhong Zhao7Chunhui Li8Guangzhong Song9Jun Yang10Lingli Sun11Jianmin Jiang12Huanying Wang13Bo Zhu14Guangyong Ye15Liang Chen16Yi-Wei Tang17Dazhi Jin18Department of Clinical Laboratory, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, AustraliaKey Laboratory of Microorganism technology and bioinformatics research of Zhejiang Province, Hangzhou, People’s Republic of ChinaCentre of Laboratory Medicine, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, People’s Republic of ChinaZhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People’s Republic of ChinaDepartment of Laboratory Medicine, Hangzhou First People’s Hospital, Hangzhou, People’s Republic of ChinaKey Laboratory of Microorganism technology and bioinformatics research of Zhejiang Province, Hangzhou, People’s Republic of ChinaDepartment of Clinical Microbiology, Second Hospital of Hebei Medical University, Hebei Provincial Center for Clinical Laboratories, Shijiazhuang, People’s Republic of ChinaInfection Control Center, Xiangya Hospital of Central South University, Changsha, People’s Republic of ChinaSchool of Laboratory Medicine, Hangzhou Medical College, Hangzhou, People’s Republic of ChinaSchool of Laboratory Medicine, Hangzhou Medical College, Hangzhou, People’s Republic of ChinaKey Laboratory of Microorganism technology and bioinformatics research of Zhejiang Province, Hangzhou, People’s Republic of ChinaZhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People’s Republic of ChinaKey Laboratory of Microorganism technology and bioinformatics research of Zhejiang Province, Hangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaCenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USACepheid, Danaher Diagnostic Platform, Shanghai, People’s Republic of ChinaSchool of Laboratory Medicine, Hangzhou Medical College, Hangzhou, People’s Republic of ChinaClostridioides difficile sequence type (ST) 37 (ribotype 017) is one of the most prevalent genotypes circulating in China. However, its genomic evolution and virulence determinants were rarely explored. Whole-genome sequencing, phylogeographic and phylogenetic analyses were conducted for C. difficile ST37 isolates. The 325 ST37 genomes from six continents, including North America (n = 66), South America (n = 4), Oceania (n = 7), Africa (n = 9), Europe (n = 138) and Asia (n = 101), were clustered into six major lineages, with region-dependent distributions, harbouring an array of antibiotic-resistance genes. The ST37 strains from China were divided into four distinct sublineages, showing five importation times and international sources. Isolates associated with severe infections exhibited significantly higher toxin productions, tcdB mRNA levels, and sporulation capacities (P < 0.001). Kyoto Encyclopedia of Genes and Genomes analysis showed 10 metabolic pathways were significantly enriched in the mutations among isolates associated with severe CDI (P < 0.05). Gene mutations in glycometabolism, amino acid metabolism and biosynthesis virtually causing instability in protein activity were correlated positively to the transcription of tcdR and negatively to the expression of toxin repressor genes, ccpA and codY. In summary, our study firstly presented genomic insights into genetic characteristics and virulence association of C. difficile ST37 in China. Gene mutations in certain important metabolic pathways are associated with severe symptoms and correlated with higher virulence in C. difficile ST37 isolates.https://www.tandfonline.com/doi/10.1080/22221751.2021.1943538Clostridioides difficileST37whole genome sequencingphylogenysevere CDI |
| spellingShingle | Xingxing Xu Yuo Luo Huan Chen Xiaojun Song Qiao Bian Xianjun Wang Qian Liang Jianhong Zhao Chunhui Li Guangzhong Song Jun Yang Lingli Sun Jianmin Jiang Huanying Wang Bo Zhu Guangyong Ye Liang Chen Yi-Wei Tang Dazhi Jin Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China Emerging Microbes and Infections Clostridioides difficile ST37 whole genome sequencing phylogeny severe CDI |
| title | Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China |
| title_full | Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China |
| title_fullStr | Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China |
| title_full_unstemmed | Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China |
| title_short | Genomic evolution and virulence association of Clostridioides difficile sequence type 37 (ribotype 017) in China |
| title_sort | genomic evolution and virulence association of clostridioides difficile sequence type 37 ribotype 017 in china |
| topic | Clostridioides difficile ST37 whole genome sequencing phylogeny severe CDI |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1943538 |
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