Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging
Optical imaging is a modality that is cost-effective, rapid, easy to use, and can be readily applied to studying disease processes and biology in vivo. For this study, we used a green fluorescent protein (GFP)- and luciferase-expressing mouse tumor model to compare and contrast the quantitative and...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03355rr02 |
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| _version_ | 1850153021321773056 |
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| author | Garry Choy Sarah O’Connor Felix E. Diehn Nick Costouros H. Richard Alexander Peter Choyke Steven K. Libutti |
| author_facet | Garry Choy Sarah O’Connor Felix E. Diehn Nick Costouros H. Richard Alexander Peter Choyke Steven K. Libutti |
| author_sort | Garry Choy |
| collection | DOAJ |
| description | Optical imaging is a modality that is cost-effective, rapid, easy to use, and can be readily applied to studying disease processes and biology in vivo. For this study, we used a green fluorescent protein (GFP)- and luciferase-expressing mouse tumor model to compare and contrast the quantitative and qualitative capabilities of a fluorescent reporter gene (GFP) and a bioluminescent reporter gene (luciferase). We describe the relationship between tumor volume, tumor mass, and bioluminescent/fluorescent intensity for both GFP and luciferase. Bioluminescent luciferase imaging was shown to be more sensitive than fluorescent GFP imaging. Luciferase-expressing tumors were detected as early as 1 day after tumor cell inoculation, whereas GFP-expressing tumors were not detected until 7 days later. Both bioluminescent and fluorescent intensity correlated significantly and linearly with tumor volume and tumor weight, as measured by caliper. Compared to bioluminescent imaging, fluorescent imaging does not require the injection of a substrate and may be appropriate for applications where sensitivity is not as critical. Knowing the relative strengths of each imaging modality will be important in guiding the decision to use fluorescence or bioluminescence. |
| format | Article |
| id | doaj-art-fb376fbbb9664cc38f13988fc2c6d7c2 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-fb376fbbb9664cc38f13988fc2c6d7c22025-08-20T02:25:50ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-11-013551022103010.2144/03355rr02Comparison of noninvasive fluorescent and bioluminescent small animal optical imagingGarry Choy0Sarah O’Connor1Felix E. Diehn2Nick Costouros3H. Richard Alexander4Peter Choyke5Steven K. Libutti61National Cancer Institute, NIH, Bethesda1National Cancer Institute, NIH, Bethesda2Warren G. Magnuson Clinical Center, NIH, Bethesda, MD, USA1National Cancer Institute, NIH, Bethesda1National Cancer Institute, NIH, Bethesda2Warren G. Magnuson Clinical Center, NIH, Bethesda, MD, USA1National Cancer Institute, NIH, BethesdaOptical imaging is a modality that is cost-effective, rapid, easy to use, and can be readily applied to studying disease processes and biology in vivo. For this study, we used a green fluorescent protein (GFP)- and luciferase-expressing mouse tumor model to compare and contrast the quantitative and qualitative capabilities of a fluorescent reporter gene (GFP) and a bioluminescent reporter gene (luciferase). We describe the relationship between tumor volume, tumor mass, and bioluminescent/fluorescent intensity for both GFP and luciferase. Bioluminescent luciferase imaging was shown to be more sensitive than fluorescent GFP imaging. Luciferase-expressing tumors were detected as early as 1 day after tumor cell inoculation, whereas GFP-expressing tumors were not detected until 7 days later. Both bioluminescent and fluorescent intensity correlated significantly and linearly with tumor volume and tumor weight, as measured by caliper. Compared to bioluminescent imaging, fluorescent imaging does not require the injection of a substrate and may be appropriate for applications where sensitivity is not as critical. Knowing the relative strengths of each imaging modality will be important in guiding the decision to use fluorescence or bioluminescence.https://www.future-science.com/doi/10.2144/03355rr02 |
| spellingShingle | Garry Choy Sarah O’Connor Felix E. Diehn Nick Costouros H. Richard Alexander Peter Choyke Steven K. Libutti Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging BioTechniques |
| title | Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging |
| title_full | Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging |
| title_fullStr | Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging |
| title_full_unstemmed | Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging |
| title_short | Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging |
| title_sort | comparison of noninvasive fluorescent and bioluminescent small animal optical imaging |
| url | https://www.future-science.com/doi/10.2144/03355rr02 |
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