Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis
Abstract Background Gene mutations may play an important role in the development, response to treatment and prognosis of colorectal cancer (CRC). This retrospective study aimed to investigate the mutation profiling of Chinese patients with CRC, and its correlation with clinicopathological features a...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.2727 |
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| author | Zu‐Lu Ye Miao‐Zhen Qiu Tao Tang Fang Wang Yi‐Xin Zhou Meng‐Jie Lei Wen‐Long Guan Cai‐Yun He |
| author_facet | Zu‐Lu Ye Miao‐Zhen Qiu Tao Tang Fang Wang Yi‐Xin Zhou Meng‐Jie Lei Wen‐Long Guan Cai‐Yun He |
| author_sort | Zu‐Lu Ye |
| collection | DOAJ |
| description | Abstract Background Gene mutations may play an important role in the development, response to treatment and prognosis of colorectal cancer (CRC). This retrospective study aimed to investigate the mutation profiling of Chinese patients with CRC, and its correlation with clinicopathological features and prognosis. Methods This study included 1190 Chinese CRC patients who were diagnosed between May 1998 and December 2018 and received clinical genetic testing. The OncoCarta Panel was used to test a total of 238 possible mutations in 19 common oncogenes. Results Five hundred and eighty‐two (48.9%) cases were detected with gene mutations. Of the 582 cases, there were 111 cases (19.7%) with two concurrent mutations, and six cases (1.0%) with three concurrent mutations. KRAS was the most common gene mutation that occurred in all cases (429, 36.1%), followed by PIK3CA (121, 10.2%), NRAS (47, 3.9%), BRAF (35, 2.9%), HRAS (11, 0.9%) and epidermal growth factor receptor (EGFR) (11, 0.9%). AKT1, KIT, FGFR1, FGFR3, FLT3, CDK, ERBB2, ABL1, MET, RET and PDGFRA mutations were also detected in several cases. When it came to prognosis, we found that KRAS/NRAS/PIK3CA/BRAF mutation was not associated with prognosis. But BRAF mutation was associated with poor prognosis in patients who accepted anti‐EGFR therapy. Conclusions The molecular testing offered the clinical data and mutation profile of Chinese CRC patients. The information of these mutated genes may help to find out the correlation between mutated genes and the development or prognosis of CRC. |
| format | Article |
| id | doaj-art-fb33df96c7804c699fc3222c00a4c6c0 |
| institution | Kabale University |
| issn | 2045-7634 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-fb33df96c7804c699fc3222c00a4c6c02025-08-25T10:14:05ZengWileyCancer Medicine2045-76342020-01-019274575610.1002/cam4.2727Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosisZu‐Lu Ye0Miao‐Zhen Qiu1Tao Tang2Fang Wang3Yi‐Xin Zhou4Meng‐Jie Lei5Wen‐Long Guan6Cai‐Yun He7Department of Molecular Diagnostics State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Medical Oncology State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Molecular Diagnostics State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Molecular Diagnostics State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of VIP State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of VIP State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Medical Oncology State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Molecular Diagnostics State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaAbstract Background Gene mutations may play an important role in the development, response to treatment and prognosis of colorectal cancer (CRC). This retrospective study aimed to investigate the mutation profiling of Chinese patients with CRC, and its correlation with clinicopathological features and prognosis. Methods This study included 1190 Chinese CRC patients who were diagnosed between May 1998 and December 2018 and received clinical genetic testing. The OncoCarta Panel was used to test a total of 238 possible mutations in 19 common oncogenes. Results Five hundred and eighty‐two (48.9%) cases were detected with gene mutations. Of the 582 cases, there were 111 cases (19.7%) with two concurrent mutations, and six cases (1.0%) with three concurrent mutations. KRAS was the most common gene mutation that occurred in all cases (429, 36.1%), followed by PIK3CA (121, 10.2%), NRAS (47, 3.9%), BRAF (35, 2.9%), HRAS (11, 0.9%) and epidermal growth factor receptor (EGFR) (11, 0.9%). AKT1, KIT, FGFR1, FGFR3, FLT3, CDK, ERBB2, ABL1, MET, RET and PDGFRA mutations were also detected in several cases. When it came to prognosis, we found that KRAS/NRAS/PIK3CA/BRAF mutation was not associated with prognosis. But BRAF mutation was associated with poor prognosis in patients who accepted anti‐EGFR therapy. Conclusions The molecular testing offered the clinical data and mutation profile of Chinese CRC patients. The information of these mutated genes may help to find out the correlation between mutated genes and the development or prognosis of CRC.https://doi.org/10.1002/cam4.2727colorectal cancermutation profilingprognosis |
| spellingShingle | Zu‐Lu Ye Miao‐Zhen Qiu Tao Tang Fang Wang Yi‐Xin Zhou Meng‐Jie Lei Wen‐Long Guan Cai‐Yun He Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis Cancer Medicine colorectal cancer mutation profiling prognosis |
| title | Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis |
| title_full | Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis |
| title_fullStr | Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis |
| title_full_unstemmed | Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis |
| title_short | Gene mutation profiling in Chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis |
| title_sort | gene mutation profiling in chinese colorectal cancer patients and its association with clinicopathological characteristics and prognosis |
| topic | colorectal cancer mutation profiling prognosis |
| url | https://doi.org/10.1002/cam4.2727 |
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