Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp.
Pretreatment of seaweed biomass for bioethanol production. This study aimed to determine the effect of biomass pretreatment with 5% NaOH alkali on enzymatic hydrolysis in the bioethanol production process using Gracilaria sp. as raw material. Pretreatment with 5% NaOH alkali was carried out with dif...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01028.pdf |
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| Summary: | Pretreatment of seaweed biomass for bioethanol production. This study aimed to determine the effect of biomass pretreatment with 5% NaOH alkali on enzymatic hydrolysis in the bioethanol production process using Gracilaria sp. as raw material. Pretreatment with 5% NaOH alkali was carried out with different time variations, namely 60, 90, and 120 minutes at a temperature of 50oC. After pretreatment, the enzymatic hydrolysis process was carried out using cellulase enzymes on a shaker incubator at a temperature of 50oC, 150 rpm for 72 hours, then the fermentation process for 72 hours using Saccharomices cerevisiae mold. The results showed that 5% NaOH alkali pretreatment could improve the quality of Gracilaria sp. biomass. The lignin content produced was 10.43 ± 0.15%. |
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| ISSN: | 2117-4458 |