Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation
Objectives. To study the short-term effect of eye opening and use of topical dexamethasone phosphate 0.1% and levofloxacin 0.5% on the cytokine levels in human tears. Methods. Prospective experimental design was used for tear collection from eyes of 10 healthy controls and 20 patients four days afte...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/570685 |
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| author | Mariann Fodor Goran Petrovski Dorottya Pásztor Péter Gogolák Éva Rajnavölgyi András Berta |
| author_facet | Mariann Fodor Goran Petrovski Dorottya Pásztor Péter Gogolák Éva Rajnavölgyi András Berta |
| author_sort | Mariann Fodor |
| collection | DOAJ |
| description | Objectives. To study the short-term effect of eye opening and use of topical dexamethasone phosphate 0.1% and levofloxacin 0.5% on the cytokine levels in human tears. Methods. Prospective experimental design was used for tear collection from eyes of 10 healthy controls and 20 patients four days after penetrating keratoplasty (PKP) at awakening and after instilling dexamethasone or levofloxacin. The concentrations of different cytokines were measured by cytometric bead array. Results. At eye opening, IL-6 levels were higher in the PKP group as compared to the controls. Thirty minutes later, the released levels of IL-10, IL-13, IL-17, IFNγ, and CCL5 increased in controls, while CXCL8 decreased in both control and PKP groups. The release of the cytokines remained stable after 30 mins except for IFNγ, which showed a decrease in the controls following levofloxacin instillation. No short-term effects of the topically used dexamethasone and levofloxacin could be detected on the cytokine levels in controls and after PKP. Conclusions. Evidence of changes in the levels and time course of tear cytokines after awakening or eye opening could be established and the short-term confounding effects of dexamethasone and levofloxacin on the levels of released cytokines in human tears could be excluded. |
| format | Article |
| id | doaj-art-fb2e3eb2163d4f3a9aa0483fa42d91e8 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-fb2e3eb2163d4f3a9aa0483fa42d91e82025-08-20T03:25:56ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/570685570685Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal TransplantationMariann Fodor0Goran Petrovski1Dorottya Pásztor2Péter Gogolák3Éva Rajnavölgyi4András Berta5Department of Ophthalmology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, HungaryDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, Szeged 6720, HungaryDepartment of Ophthalmology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, HungaryDepartment of Immunology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, HungaryDepartment of Immunology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, HungaryDepartment of Ophthalmology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, HungaryObjectives. To study the short-term effect of eye opening and use of topical dexamethasone phosphate 0.1% and levofloxacin 0.5% on the cytokine levels in human tears. Methods. Prospective experimental design was used for tear collection from eyes of 10 healthy controls and 20 patients four days after penetrating keratoplasty (PKP) at awakening and after instilling dexamethasone or levofloxacin. The concentrations of different cytokines were measured by cytometric bead array. Results. At eye opening, IL-6 levels were higher in the PKP group as compared to the controls. Thirty minutes later, the released levels of IL-10, IL-13, IL-17, IFNγ, and CCL5 increased in controls, while CXCL8 decreased in both control and PKP groups. The release of the cytokines remained stable after 30 mins except for IFNγ, which showed a decrease in the controls following levofloxacin instillation. No short-term effects of the topically used dexamethasone and levofloxacin could be detected on the cytokine levels in controls and after PKP. Conclusions. Evidence of changes in the levels and time course of tear cytokines after awakening or eye opening could be established and the short-term confounding effects of dexamethasone and levofloxacin on the levels of released cytokines in human tears could be excluded.http://dx.doi.org/10.1155/2014/570685 |
| spellingShingle | Mariann Fodor Goran Petrovski Dorottya Pásztor Péter Gogolák Éva Rajnavölgyi András Berta Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation Journal of Immunology Research |
| title | Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation |
| title_full | Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation |
| title_fullStr | Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation |
| title_full_unstemmed | Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation |
| title_short | Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation |
| title_sort | effects of awakening and the use of topical dexamethasone and levofloxacin on the cytokine levels in tears following corneal transplantation |
| url | http://dx.doi.org/10.1155/2014/570685 |
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