Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour

ABSTRACT The reproductive systems of natural populations can significantly impact their genetic diversity by either preventing or promoting inbreeding. Therefore, it is crucial to have a comprehensive understanding of the mating system to evaluate a population's ability to maintain genetic dive...

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Main Authors: Violaine Dolfo, Cécile Gaspar, Miri Tatarata, Emilie Boissin, Serge Planes
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.70855
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author Violaine Dolfo
Cécile Gaspar
Miri Tatarata
Emilie Boissin
Serge Planes
author_facet Violaine Dolfo
Cécile Gaspar
Miri Tatarata
Emilie Boissin
Serge Planes
author_sort Violaine Dolfo
collection DOAJ
description ABSTRACT The reproductive systems of natural populations can significantly impact their genetic diversity by either preventing or promoting inbreeding. Therefore, it is crucial to have a comprehensive understanding of the mating system to evaluate a population's ability to maintain genetic diversity over time. In this study, we examine the mating system of an endangered population of green sea turtles in Tetiaroa, French Polynesia. We determine if different mating behaviours serve as strategies to avoid inbreeding. We genotyped 107 nesting females and 1483 hatchlings from 549 nests and used 23 microsatellite markers to reconstruct the genotypes of the sires. We assessed the level of inbreeding and relatedness among the parent pairs and explored the correlation between relatedness and indicators of reproductive success. We investigated the mating behaviours of both males and females and determined whether specific behaviours were linked to different levels of relatedness. We explored for the first time the relatedness bias of mating behaviours in green turtles. Our results showed that the global Fis was significant in the population, and the levels of relatedness were higher than expected through random mating, indicating inbreeding and non‐random partner selection for related mates. No mating behaviours were associated with lower relatedness levels, suggesting inbreeding tolerance or preference in this population. Finally, we discovered unexpected plasticity in the reproductive frequency of females, the length of the inter‐nesting interval, and the relative timing of breeding and nesting. If confirmed in other populations and with a larger sample size, these new findings may reshape our understanding of the green turtle's intricate reproductive system.
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spelling doaj-art-fb2b2f98ff49403599c3e66ef1d5b1ce2025-08-20T03:35:01ZengWileyEcology and Evolution2045-77582025-07-01157n/an/a10.1002/ece3.70855Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive BehaviourViolaine Dolfo0Cécile Gaspar1Miri Tatarata2Emilie Boissin3Serge Planes4PSL Research University: EPHE–UPVD–CNRS UAR 3278 CRIOBE Perpignan FranceTe Mana O Te Moana Foundation Faaa French PolynesiaDirection de l'Environnement de la Polynésie Française Papeete French PolynesiaPSL Research University: EPHE–UPVD–CNRS UAR 3278 CRIOBE Perpignan FrancePSL Research University: EPHE–UPVD–CNRS UAR 3278 CRIOBE Perpignan FranceABSTRACT The reproductive systems of natural populations can significantly impact their genetic diversity by either preventing or promoting inbreeding. Therefore, it is crucial to have a comprehensive understanding of the mating system to evaluate a population's ability to maintain genetic diversity over time. In this study, we examine the mating system of an endangered population of green sea turtles in Tetiaroa, French Polynesia. We determine if different mating behaviours serve as strategies to avoid inbreeding. We genotyped 107 nesting females and 1483 hatchlings from 549 nests and used 23 microsatellite markers to reconstruct the genotypes of the sires. We assessed the level of inbreeding and relatedness among the parent pairs and explored the correlation between relatedness and indicators of reproductive success. We investigated the mating behaviours of both males and females and determined whether specific behaviours were linked to different levels of relatedness. We explored for the first time the relatedness bias of mating behaviours in green turtles. Our results showed that the global Fis was significant in the population, and the levels of relatedness were higher than expected through random mating, indicating inbreeding and non‐random partner selection for related mates. No mating behaviours were associated with lower relatedness levels, suggesting inbreeding tolerance or preference in this population. Finally, we discovered unexpected plasticity in the reproductive frequency of females, the length of the inter‐nesting interval, and the relative timing of breeding and nesting. If confirmed in other populations and with a larger sample size, these new findings may reshape our understanding of the green turtle's intricate reproductive system.https://doi.org/10.1002/ece3.70855genotype reconstructioninbreedingmating behaviourmultiple paternityrelatednessreproductive success
spellingShingle Violaine Dolfo
Cécile Gaspar
Miri Tatarata
Emilie Boissin
Serge Planes
Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour
Ecology and Evolution
genotype reconstruction
inbreeding
mating behaviour
multiple paternity
relatedness
reproductive success
title Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour
title_full Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour
title_fullStr Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour
title_full_unstemmed Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour
title_short Parentage Analysis in a Green Sea Turtle (Chelonia mydas) Population From French Polynesia Reveals a Tendency for Inbreeding and Unexpected Plasticity in Reproductive Behaviour
title_sort parentage analysis in a green sea turtle chelonia mydas population from french polynesia reveals a tendency for inbreeding and unexpected plasticity in reproductive behaviour
topic genotype reconstruction
inbreeding
mating behaviour
multiple paternity
relatedness
reproductive success
url https://doi.org/10.1002/ece3.70855
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