Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals
Alteration of the gut microbiota (GM) is associated with various diseases, including colorectal cancer (CRC). With the development of next-generation sequencing techniques, metagenomic sequencing, along with metabolic function and antibiotic-resistant gene analyses, has been used to investigate diff...
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| Language: | English |
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2025-03-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://doi.org/10.33073/pjm-2025-006 |
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| author | Li Jun Zhu Yanyun Chang Qing Gong Yuan Wan Jun Xu Shiping |
| author_facet | Li Jun Zhu Yanyun Chang Qing Gong Yuan Wan Jun Xu Shiping |
| author_sort | Li Jun |
| collection | DOAJ |
| description | Alteration of the gut microbiota (GM) is associated with various diseases, including colorectal cancer (CRC). With the development of next-generation sequencing techniques, metagenomic sequencing, along with metabolic function and antibiotic-resistant gene analyses, has been used to investigate differences in GM between CRC patients and healthy controls. Fecal samples were obtained from seven CRC patients and six healthy subjects, and the sequencing data were analyzed for similarity, a-diversity, principal component analysis (PCA), and linear discriminant analyses (LDA). Regarding Actinobacteria, 3 orders, 5 families, 9 genera, and 19 species were identified with no differences between the CRC and control groups, while the levels of Bifidobacterium bifidum and Bifidobacterium dentium were higher, and the level of Bifidobacterium breve was lower in the CRC group compared to the healthy controls (p = 0.053). Otherwise, 2 genera (Leuco-nostoc and Salmonella) and 7 species of bacteria (Parabacteroides merdae, Alistipes shahii, Alistipes finegoldii, Clostridium nexile, Salmonella enterica, unclassified Salmonella, Enterobacter cloacae) were found to be significantly differently distributed between CRC patients and healthy controls. PCA-LDA successfully classified these 2 groups with satisfactory accuracy (84.52% for metabolic function and 77.38% for resistant genes). These findings underscore the potential of GM as a diagnostic tool for CRC, offering a promising avenue for non-invasive screening and risk assessment. The identification of specific microbial signatures, particularly those linked to metabolic functions and resistance traits, could open new doors for understanding the role of the microbiome in CRC progression and treatment resistance. |
| format | Article |
| id | doaj-art-fb2aaf6655bc4db79563df7a0e1fe640 |
| institution | Kabale University |
| issn | 2544-4646 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj-art-fb2aaf6655bc4db79563df7a0e1fe6402025-08-20T03:41:04ZengSciendoPolish Journal of Microbiology2544-46462025-03-01741718110.33073/pjm-2025-006Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy IndividualsLi Jun0Zhu Yanyun1Chang Qing2Gong Yuan3Wan Jun4Xu Shiping5Department of Gastroenterology, Second Medical Center, Chinese People’s Liberation Army General Hospital, Beijing, ChinaDepartment of Oncology, First Medical Center, Chinese People’s Liberation Army General Hospital, Beijing, ChinaDepartment of Gastroenterology, Second Medical Center, Chinese People’s Liberation Army General Hospital, Beijing, ChinaDepartment of Gastroenterology, Second Medical Center, Chinese People’s Liberation Army General Hospital, Beijing, ChinaDepartment of Gastroenterology, Second Medical Center, Chinese People’s Liberation Army General Hospital, Beijing, ChinaDepartment of Gastroenterology, Second Medical Center, Chinese People’s Liberation Army General Hospital, Beijing, ChinaAlteration of the gut microbiota (GM) is associated with various diseases, including colorectal cancer (CRC). With the development of next-generation sequencing techniques, metagenomic sequencing, along with metabolic function and antibiotic-resistant gene analyses, has been used to investigate differences in GM between CRC patients and healthy controls. Fecal samples were obtained from seven CRC patients and six healthy subjects, and the sequencing data were analyzed for similarity, a-diversity, principal component analysis (PCA), and linear discriminant analyses (LDA). Regarding Actinobacteria, 3 orders, 5 families, 9 genera, and 19 species were identified with no differences between the CRC and control groups, while the levels of Bifidobacterium bifidum and Bifidobacterium dentium were higher, and the level of Bifidobacterium breve was lower in the CRC group compared to the healthy controls (p = 0.053). Otherwise, 2 genera (Leuco-nostoc and Salmonella) and 7 species of bacteria (Parabacteroides merdae, Alistipes shahii, Alistipes finegoldii, Clostridium nexile, Salmonella enterica, unclassified Salmonella, Enterobacter cloacae) were found to be significantly differently distributed between CRC patients and healthy controls. PCA-LDA successfully classified these 2 groups with satisfactory accuracy (84.52% for metabolic function and 77.38% for resistant genes). These findings underscore the potential of GM as a diagnostic tool for CRC, offering a promising avenue for non-invasive screening and risk assessment. The identification of specific microbial signatures, particularly those linked to metabolic functions and resistance traits, could open new doors for understanding the role of the microbiome in CRC progression and treatment resistance.https://doi.org/10.33073/pjm-2025-006actinobacteriaantibiotic resistant genescolorectal cancergut microbiotametagenomics |
| spellingShingle | Li Jun Zhu Yanyun Chang Qing Gong Yuan Wan Jun Xu Shiping Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals Polish Journal of Microbiology actinobacteria antibiotic resistant genes colorectal cancer gut microbiota metagenomics |
| title | Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals |
| title_full | Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals |
| title_fullStr | Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals |
| title_full_unstemmed | Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals |
| title_short | Comparative Analysis of Microbiological Profiles and Antibiotic Resistance Genes in Subjects with Colorectal Cancer and Healthy Individuals |
| title_sort | comparative analysis of microbiological profiles and antibiotic resistance genes in subjects with colorectal cancer and healthy individuals |
| topic | actinobacteria antibiotic resistant genes colorectal cancer gut microbiota metagenomics |
| url | https://doi.org/10.33073/pjm-2025-006 |
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