Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance
Heat shock proteins 90 (HSP90s) are conserved molecular chaperones essential for cellular homeostasis under abiotic stress. While several studies have been reported to elucidate the role of HSP90s in heat stress responses in cotton, their involvement in salt stress remains underexplored. Gossypium h...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1574604/full |
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| author | Zhao Yan Hao Zhao Yan Hao Qian Feng Xu Yan Man Dong Qi Qi Yu Shi Qing Zhai Wei Yang Mohssen Elbagory Esam Sayed Kasem Muhammad Yasir Jiang Yu Rong |
| author_facet | Zhao Yan Hao Zhao Yan Hao Qian Feng Xu Yan Man Dong Qi Qi Yu Shi Qing Zhai Wei Yang Mohssen Elbagory Esam Sayed Kasem Muhammad Yasir Jiang Yu Rong |
| author_sort | Zhao Yan Hao |
| collection | DOAJ |
| description | Heat shock proteins 90 (HSP90s) are conserved molecular chaperones essential for cellular homeostasis under abiotic stress. While several studies have been reported to elucidate the role of HSP90s in heat stress responses in cotton, their involvement in salt stress remains underexplored. Gossypium hirsutum L. is highly susceptible to salt stress. The Current study investigated the evolutionary aspects, expression patterns, and functional validation of HSP90 family members in cotton under salt stress. A comprehensive genomic analysis of G. hirsutum, G. raimondii, and G. arboreum, identified 56 HSP90 genes which were classified into three distinct phylogenetic groups. Gene structure and motifs analysis revealed a conserved nature of HSP90s within each group. Additionally, cis-acting elements suggested the potential roles of HSP90s in biotic and abiotic stresses. The Ka/Ks ratio of all genes was < 1 suggesting negative and purifying selection pressure during molecular evolution. Expression analysis demonstrated the potential role of HSP90 genes in salt tolerance. Notably, out of ten HSP90 genes five genes exhibited highly differential expression under salt stress, as confirmed by qRT-PCR analysis. Moreover, virus-induced silencing of the two salt stress-responsive genes, Ghir_D03G016230 and Ghir_D02G013530, which were upregulated under salt stress, resulted in a significant decrease in SOD, POD, and CAT, accompanied by a marked increase in MDA content in the salt-tolerant cotton cultivar. These findings provide novel insights into the functional roles of HSP90s in G. hirsutum under salt stress. |
| format | Article |
| id | doaj-art-fb25f44ac2dd4d628932e8d916ff0c8c |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-fb25f44ac2dd4d628932e8d916ff0c8c2025-08-20T03:28:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-07-011610.3389/fpls.2025.15746041574604Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress toleranceZhao Yan Hao0Zhao Yan Hao1Qian Feng2Xu Yan Man3Dong Qi Qi4Yu Shi Qing5Zhai Wei Yang6Mohssen Elbagory7Esam Sayed Kasem8Muhammad Yasir9Jiang Yu Rong10College of Advanced Agricultural Science, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaAgricultural Extension Department, Tonglu County Agricultural Technology Extension Center, Hangzhou, ChinaCollege of Advanced Agricultural Science, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaJixian Honors College, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaFinancial Management Office of Sixi Town People's Government, Taishun County, Wenzhou, Zhejiang, ChinaCollege of Advanced Agricultural Science, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaCollege of Advanced Agricultural Science, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaHealth Specialties, Basic Sciences and Applications Unit, Applied College, King Khalid University, Mohail Assir, Saudi ArabiaSoil, Water and Environment Research Institute, Agriculture Research Center, Giza, EgyptCollege of Tea Science and Tea Culture, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaCollege of Advanced Agricultural Science, Zhejiang Agriculture and Forestry University, Hangzhou, ChinaHeat shock proteins 90 (HSP90s) are conserved molecular chaperones essential for cellular homeostasis under abiotic stress. While several studies have been reported to elucidate the role of HSP90s in heat stress responses in cotton, their involvement in salt stress remains underexplored. Gossypium hirsutum L. is highly susceptible to salt stress. The Current study investigated the evolutionary aspects, expression patterns, and functional validation of HSP90 family members in cotton under salt stress. A comprehensive genomic analysis of G. hirsutum, G. raimondii, and G. arboreum, identified 56 HSP90 genes which were classified into three distinct phylogenetic groups. Gene structure and motifs analysis revealed a conserved nature of HSP90s within each group. Additionally, cis-acting elements suggested the potential roles of HSP90s in biotic and abiotic stresses. The Ka/Ks ratio of all genes was < 1 suggesting negative and purifying selection pressure during molecular evolution. Expression analysis demonstrated the potential role of HSP90 genes in salt tolerance. Notably, out of ten HSP90 genes five genes exhibited highly differential expression under salt stress, as confirmed by qRT-PCR analysis. Moreover, virus-induced silencing of the two salt stress-responsive genes, Ghir_D03G016230 and Ghir_D02G013530, which were upregulated under salt stress, resulted in a significant decrease in SOD, POD, and CAT, accompanied by a marked increase in MDA content in the salt-tolerant cotton cultivar. These findings provide novel insights into the functional roles of HSP90s in G. hirsutum under salt stress.https://www.frontiersin.org/articles/10.3389/fpls.2025.1574604/fullGossypium hirsutumHsp90salt stressVIGScotton |
| spellingShingle | Zhao Yan Hao Zhao Yan Hao Qian Feng Xu Yan Man Dong Qi Qi Yu Shi Qing Zhai Wei Yang Mohssen Elbagory Esam Sayed Kasem Muhammad Yasir Jiang Yu Rong Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance Frontiers in Plant Science Gossypium hirsutum Hsp90 salt stress VIGS cotton |
| title | Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance |
| title_full | Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance |
| title_fullStr | Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance |
| title_full_unstemmed | Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance |
| title_short | Genome-wide identification and characterization of HSP90 family gene in cotton and their potential role in salt stress tolerance |
| title_sort | genome wide identification and characterization of hsp90 family gene in cotton and their potential role in salt stress tolerance |
| topic | Gossypium hirsutum Hsp90 salt stress VIGS cotton |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1574604/full |
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