Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA
Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2017-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/8363 |
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| author | Sibel Aydogan Ziya Cibal Acikgoz Aysegul Gozalan Fisun Kirca Tuba Muderris Elife Ozturk |
| author_facet | Sibel Aydogan Ziya Cibal Acikgoz Aysegul Gozalan Fisun Kirca Tuba Muderris Elife Ozturk |
| author_sort | Sibel Aydogan |
| collection | DOAJ |
| description |
Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections.
Methodology: The study was conducted on 121 and 54 clinical samples for the detection of HBV DNA and HCV RNA, respectively, with an additional 8 HCV RNA external quality control samples.
Results: Though a high correlation was observed between the two kits for the HBV DNA (r = 0.955, p = 0.001) and HCV RNA quantifications (r = 0.828, p = 0.001), discordant results were found in nine of the HBV DNA and in six of the HCV RNA samples. The mean difference between the two systems was found to be 0.4 log IU/mL in Quality Control for Molecular Diagnostics (QCMD) HCV RNA samples by Bland-Altman analysis.
Conclusions: Although there was a high correlation between HBV DNA and HCV RNA tests according to the results of the study, the RTA system requires improvement for the determination of HCV RNA.
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| format | Article |
| id | doaj-art-fb21b4c9511e4851a4e4d0cfe7a14936 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fb21b4c9511e4851a4e4d0cfe7a149362025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-07-01110710.3855/jidc.8363Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNASibel Aydogan0Ziya Cibal Acikgoz1Aysegul Gozalan2Fisun Kirca3Tuba Muderris4Elife Ozturk5Ankara Ataturk Training and Research Hospital, Ankara, TurkeyFaculty of Medicine, Yıldırım Beyazıt University, Ankara, TurkeyAnkara Ataturk Training and Research Hospital, Ankara, TurkeyAnkara Ataturk Training and Research Hospital, Ankara, TurkeyAnkara Ataturk Training and Research Hospital, Ankara, TurkeyAnkara Ataturk Training and Research Hospital, Ankara, Turkey Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. Methodology: The study was conducted on 121 and 54 clinical samples for the detection of HBV DNA and HCV RNA, respectively, with an additional 8 HCV RNA external quality control samples. Results: Though a high correlation was observed between the two kits for the HBV DNA (r = 0.955, p = 0.001) and HCV RNA quantifications (r = 0.828, p = 0.001), discordant results were found in nine of the HBV DNA and in six of the HCV RNA samples. The mean difference between the two systems was found to be 0.4 log IU/mL in Quality Control for Molecular Diagnostics (QCMD) HCV RNA samples by Bland-Altman analysis. Conclusions: Although there was a high correlation between HBV DNA and HCV RNA tests according to the results of the study, the RTA system requires improvement for the determination of HCV RNA. https://jidc.org/index.php/journal/article/view/8363Artus RGreal time PCRRTAviral hepatitisreal-time PCRspin column |
| spellingShingle | Sibel Aydogan Ziya Cibal Acikgoz Aysegul Gozalan Fisun Kirca Tuba Muderris Elife Ozturk Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA Journal of Infection in Developing Countries Artus RG real time PCR RTA viral hepatitis real-time PCR spin column |
| title | Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA |
| title_full | Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA |
| title_fullStr | Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA |
| title_full_unstemmed | Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA |
| title_short | Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA |
| title_sort | comparison of a novel real time pcr method rta and artus rg for the quantification of hbv dna and hcv rna |
| topic | Artus RG real time PCR RTA viral hepatitis real-time PCR spin column |
| url | https://jidc.org/index.php/journal/article/view/8363 |
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