Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells
Objective To explore Aliskiren on transdifferentiation of renal tubular epithelial cells（ H KC） induced by TGF-β<sub>1</sub> and to evaluate its effect on platelet derived growth factor（ PDGF-BB） 、vascular endothelialgrowth factor（ VEGF） expressing change. M ethods H KC were divided into...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2018-01-01
|
| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57915615&Fpath=home&index=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850143260518907904 |
|---|---|
| author | HUANG Jian CHEN Song YANG Jing MIN Ya-li |
| author_facet | HUANG Jian CHEN Song YANG Jing MIN Ya-li |
| author_sort | HUANG Jian |
| collection | DOAJ |
| description | Objective To explore Aliskiren on transdifferentiation of renal tubular epithelial cells（ H KC） induced by TGF-β<sub>1</sub> and to evaluate its effect on platelet derived growth factor（ PDGF-BB） 、vascular endothelialgrowth factor（ VEGF） expressing change. M ethods H KC were divided into four groups and cultured for 2 4 hours in different conditions separately : Serum free as control,Treated with Aliskiren( 1. 0 × 1 0<sup>-7</sup> m m ol/L); Treated with TGF-β<sub>1</sub>（ 8 ng/ml）; Treated with TGF-β<sub>1</sub> plus Aliskiren in the sam e concentration. The expression ofα-SMA was assessed with RT-PCR,the expression of PDGF-BB、VEGF was assessed with RTPCR and im m unohistochem istry after 2 4 hours. Results There are little expression of PDGFBB,VEGF and α-SM A m RNA in control group. Com pared with the control group,The expression of PDGF-BB and α-SM A m RNA was m arkedly increased in H CK cultured with TGF-β<sub>1</sub> group（ P < 0. 0 5）,which were suppressed significantly in H KC cultured with TGF-β<sub>1</sub> plus Aliskiren group（ P < 0. 0 5）. but there are no significant changes in the expression of VEGFbetween different groups（ P > 0. 0 5）. Conclusions Aliskiren may inhibit transdifferentiation of tubular epithelial cells induced by TGF-β<sub>1</sub> and reduce the expression of PDGF-BB. The inhibition of Aliskiren on transdifferentiation of tubular epithelial cells may be related to the down-regulation of PDGFBB. It is not very clear on the effect of the expression of VEGF. |
| format | Article |
| id | doaj-art-fb1bcddf185a49c08276e2c0f0896c23 |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2018-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-fb1bcddf185a49c08276e2c0f0896c232025-08-20T02:28:46ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902018-01-0146647057915615Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cellsHUANG JianCHEN SongYANG JingMIN Ya-liObjective To explore Aliskiren on transdifferentiation of renal tubular epithelial cells（ H KC） induced by TGF-β<sub>1</sub> and to evaluate its effect on platelet derived growth factor（ PDGF-BB） 、vascular endothelialgrowth factor（ VEGF） expressing change. M ethods H KC were divided into four groups and cultured for 2 4 hours in different conditions separately : Serum free as control,Treated with Aliskiren( 1. 0 × 1 0<sup>-7</sup> m m ol/L); Treated with TGF-β<sub>1</sub>（ 8 ng/ml）; Treated with TGF-β<sub>1</sub> plus Aliskiren in the sam e concentration. The expression ofα-SMA was assessed with RT-PCR,the expression of PDGF-BB、VEGF was assessed with RTPCR and im m unohistochem istry after 2 4 hours. Results There are little expression of PDGFBB,VEGF and α-SM A m RNA in control group. Com pared with the control group,The expression of PDGF-BB and α-SM A m RNA was m arkedly increased in H CK cultured with TGF-β<sub>1</sub> group（ P < 0. 0 5）,which were suppressed significantly in H KC cultured with TGF-β<sub>1</sub> plus Aliskiren group（ P < 0. 0 5）. but there are no significant changes in the expression of VEGFbetween different groups（ P > 0. 0 5）. Conclusions Aliskiren may inhibit transdifferentiation of tubular epithelial cells induced by TGF-β<sub>1</sub> and reduce the expression of PDGF-BB. The inhibition of Aliskiren on transdifferentiation of tubular epithelial cells may be related to the down-regulation of PDGFBB. It is not very clear on the effect of the expression of VEGF.http://www.lcszb.com/thesisDetails?columnId=57915615&Fpath=home&index=0AliskirenTransdifferentiationPDGF-BBVEGF |
| spellingShingle | HUANG Jian CHEN Song YANG Jing MIN Ya-li Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells Linchuang shenzangbing zazhi Aliskiren Transdifferentiation PDGF-BB VEGF |
| title | Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells |
| title_full | Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells |
| title_fullStr | Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells |
| title_full_unstemmed | Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells |
| title_short | Effects of Aliskiren on transdifferentiation induced by TGF-β<sub>1</sub> in human renal tubular epithelial cells |
| title_sort | effects of aliskiren on transdifferentiation induced by tgf β sub 1 sub in human renal tubular epithelial cells |
| topic | Aliskiren Transdifferentiation PDGF-BB VEGF |
| url | http://www.lcszb.com/thesisDetails?columnId=57915615&Fpath=home&index=0 |
| work_keys_str_mv | AT huangjian effectsofaliskirenontransdifferentiationinducedbytgfbsub1subinhumanrenaltubularepithelialcells AT chensong effectsofaliskirenontransdifferentiationinducedbytgfbsub1subinhumanrenaltubularepithelialcells AT yangjing effectsofaliskirenontransdifferentiationinducedbytgfbsub1subinhumanrenaltubularepithelialcells AT minyali effectsofaliskirenontransdifferentiationinducedbytgfbsub1subinhumanrenaltubularepithelialcells |