A unified-field theory of genome organization and gene regulation

Summary: Our aim is to predict how often genic and non-genic promoters fire within a cell. We first review a parsimonious pan-genomic model for genome organization and gene regulation, where transcription rate is determined by proximity in 3D space of promoters to clusters containing appropriate fac...

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Main Authors: Giuseppe Negro, Massimiliano Semeraro, Peter R. Cook, Davide Marenduzzo
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:iScience
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Online Access:http://www.sciencedirect.com/science/article/pii/S258900422402443X
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author Giuseppe Negro
Massimiliano Semeraro
Peter R. Cook
Davide Marenduzzo
author_facet Giuseppe Negro
Massimiliano Semeraro
Peter R. Cook
Davide Marenduzzo
author_sort Giuseppe Negro
collection DOAJ
description Summary: Our aim is to predict how often genic and non-genic promoters fire within a cell. We first review a parsimonious pan-genomic model for genome organization and gene regulation, where transcription rate is determined by proximity in 3D space of promoters to clusters containing appropriate factors and RNA polymerases. This model reconciles conflicting results indicating that regulatory mammalian networks are both simple (as over-expressing just 4 transcription factors switches cell state) and complex (as genome-wide association studies show phenotypes like cell type are determined by thousands of loci rarely encoding such factors). We then present 3D polymer simulations, and a proximity formula based on our biological model that enables prediction of transcriptional activities of all promoters in three human cell types. This simple fitting-free formula contains just one variable (distance on the genetic map to the nearest active promoter), and we suggest it can in principle be applied to any organism.
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spelling doaj-art-fb1829e5ebdb4f8d9123af618cfe3fff2025-08-20T02:35:05ZengElsevieriScience2589-00422024-12-01271211121810.1016/j.isci.2024.111218A unified-field theory of genome organization and gene regulationGiuseppe Negro0Massimiliano Semeraro1Peter R. Cook2Davide Marenduzzo3SUPA, School of Physics, University of Edinburgh, Peter Guthrie Tait Road, Edinburgh EH9 3FD, UK; Dipartimento Interateneo di Fisica, Università degli Studi di Bari and INFN, Sezione di Bari, Via Amendola 173, I-70126 Bari, ItalyDipartimento Interateneo di Fisica, Università degli Studi di Bari and INFN, Sezione di Bari, Via Amendola 173, I-70126 Bari, ItalySir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK; Corresponding authorSUPA, School of Physics, University of Edinburgh, Peter Guthrie Tait Road, Edinburgh EH9 3FD, UK; Corresponding authorSummary: Our aim is to predict how often genic and non-genic promoters fire within a cell. We first review a parsimonious pan-genomic model for genome organization and gene regulation, where transcription rate is determined by proximity in 3D space of promoters to clusters containing appropriate factors and RNA polymerases. This model reconciles conflicting results indicating that regulatory mammalian networks are both simple (as over-expressing just 4 transcription factors switches cell state) and complex (as genome-wide association studies show phenotypes like cell type are determined by thousands of loci rarely encoding such factors). We then present 3D polymer simulations, and a proximity formula based on our biological model that enables prediction of transcriptional activities of all promoters in three human cell types. This simple fitting-free formula contains just one variable (distance on the genetic map to the nearest active promoter), and we suggest it can in principle be applied to any organism.http://www.sciencedirect.com/science/article/pii/S258900422402443XNatural sciencesBiological sciencesSystems biologyData processing in systems biology
spellingShingle Giuseppe Negro
Massimiliano Semeraro
Peter R. Cook
Davide Marenduzzo
A unified-field theory of genome organization and gene regulation
iScience
Natural sciences
Biological sciences
Systems biology
Data processing in systems biology
title A unified-field theory of genome organization and gene regulation
title_full A unified-field theory of genome organization and gene regulation
title_fullStr A unified-field theory of genome organization and gene regulation
title_full_unstemmed A unified-field theory of genome organization and gene regulation
title_short A unified-field theory of genome organization and gene regulation
title_sort unified field theory of genome organization and gene regulation
topic Natural sciences
Biological sciences
Systems biology
Data processing in systems biology
url http://www.sciencedirect.com/science/article/pii/S258900422402443X
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