Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model

Background Periodontitis is a widespread chronic inflammatory disease impacting 20–50% of the global population.Methods This study evaluated the effects of Weissella cibaria CMU (CMU) on preventing the progression of periodontitis in a rat model. Periodontitis was induced by injecting lipopolysaccha...

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Main Authors: Kyung-Hyo Do, Soyoung Boo, Rayoung You, Sanggu Kim, Soochong Kim, Kwang-Won Seo, Mi-Sun Kang, Wan-Kyu Lee
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Oral Microbiology
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Online Access:https://www.tandfonline.com/doi/10.1080/20002297.2025.2469895
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author Kyung-Hyo Do
Soyoung Boo
Rayoung You
Sanggu Kim
Soochong Kim
Kwang-Won Seo
Mi-Sun Kang
Wan-Kyu Lee
author_facet Kyung-Hyo Do
Soyoung Boo
Rayoung You
Sanggu Kim
Soochong Kim
Kwang-Won Seo
Mi-Sun Kang
Wan-Kyu Lee
author_sort Kyung-Hyo Do
collection DOAJ
description Background Periodontitis is a widespread chronic inflammatory disease impacting 20–50% of the global population.Methods This study evaluated the effects of Weissella cibaria CMU (CMU) on preventing the progression of periodontitis in a rat model. Periodontitis was induced by injecting lipopolysaccharide into the palatal gingiva around the first and second maxillary molars. CMU was then administered at three concentrations (low: 2 × 107 CFU/rat/day; mid: 2 × 108 CFU/rat/day; high: 2 × 109 CFU/rat/day) for 14 days to assess its ability to prevent further periodontal damage.Results The administration of CMU significantly improved gingivitis and plaque indices in a dose-dependent manner. Macroscopic analysis and micro-computed tomography showed a dose-dependent reduction in alveolar bone loss in the CMU groups. Although histopathological analysis indicated a decrease in bone loss, statistical significance was not achieved in the high-dose group. Pro-inflammatory mediators such as TNF-α, IL-6, MMP-1, and MMP-9 were suppressed in a dose-dependent manner in the CMU groups. Additionally, mid- and high-dose CMU increased the relative abundance of Weissella in the oral microbiome.Conclusions CMU can influence the oral microbiome, reduce inflammatory mediators, and alleviate histological changes in periodontal tissue, highlighting its potential as a probiotic strain for preventing periodontitis.
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spelling doaj-art-fb13407ca51b42468fbb07e2b0e333b92025-08-20T02:30:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2025.2469895Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat modelKyung-Hyo Do0Soyoung Boo1Rayoung You2Sanggu Kim3Soochong Kim4Kwang-Won Seo5Mi-Sun Kang6Wan-Kyu Lee7College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Pathology and Platelet Signaling, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaLaboratory of Veterinary Pathology and Platelet Signaling, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaR&D Center, OraTicx Inc., Seoul, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaBackground Periodontitis is a widespread chronic inflammatory disease impacting 20–50% of the global population.Methods This study evaluated the effects of Weissella cibaria CMU (CMU) on preventing the progression of periodontitis in a rat model. Periodontitis was induced by injecting lipopolysaccharide into the palatal gingiva around the first and second maxillary molars. CMU was then administered at three concentrations (low: 2 × 107 CFU/rat/day; mid: 2 × 108 CFU/rat/day; high: 2 × 109 CFU/rat/day) for 14 days to assess its ability to prevent further periodontal damage.Results The administration of CMU significantly improved gingivitis and plaque indices in a dose-dependent manner. Macroscopic analysis and micro-computed tomography showed a dose-dependent reduction in alveolar bone loss in the CMU groups. Although histopathological analysis indicated a decrease in bone loss, statistical significance was not achieved in the high-dose group. Pro-inflammatory mediators such as TNF-α, IL-6, MMP-1, and MMP-9 were suppressed in a dose-dependent manner in the CMU groups. Additionally, mid- and high-dose CMU increased the relative abundance of Weissella in the oral microbiome.Conclusions CMU can influence the oral microbiome, reduce inflammatory mediators, and alleviate histological changes in periodontal tissue, highlighting its potential as a probiotic strain for preventing periodontitis.https://www.tandfonline.com/doi/10.1080/20002297.2025.2469895Periodontal disease(s)/periodontitisoral hygieneprobioticsmicrobiomeinflammationmicrobiology
spellingShingle Kyung-Hyo Do
Soyoung Boo
Rayoung You
Sanggu Kim
Soochong Kim
Kwang-Won Seo
Mi-Sun Kang
Wan-Kyu Lee
Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
Journal of Oral Microbiology
Periodontal disease(s)/periodontitis
oral hygiene
probiotics
microbiome
inflammation
microbiology
title Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
title_full Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
title_fullStr Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
title_full_unstemmed Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
title_short Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
title_sort preventive effects of weissella cibaria cmu on the progression of periodontitis in a rat model
topic Periodontal disease(s)/periodontitis
oral hygiene
probiotics
microbiome
inflammation
microbiology
url https://www.tandfonline.com/doi/10.1080/20002297.2025.2469895
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