Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats
ABSTRACT Purpose: To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis. Methods: Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by bioc...
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2025-03-01
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| Series: | Acta Cirúrgica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100209&tlng=en |
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| author | Hui Zhang Junfang Dong Jianwu Zhang Hongxue Chen Ting Liu Ruixue Gan Jing Wen Yangyou Li |
| author_facet | Hui Zhang Junfang Dong Jianwu Zhang Hongxue Chen Ting Liu Ruixue Gan Jing Wen Yangyou Li |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | ABSTRACT Purpose: To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis. Methods: Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by biochemical assay kit. Detection of apoptosis was by flow cytometry. Serum levels of creatine kinase isoenzyme MB (CK-MB), LDH, and cardiac troponin I (cTnI) were detected by enzyme-linked immunosorbent assay. Myocardial infarction area and pathological changes were observed via 2,3,5-triphenyltetrazolium chloride (TTC) staining and hematoxylin and eosin staining. The expressions of apoptosis-related proteins in cells and myocardial tissues were detected by Western blot. Results: H9c2 cell viability was significantly increased by pretreatment with 16 and 32 μg/mL of borneol. Borneol pretreatment significantly increased the T-SOD levels and reduced LDH leakage and apoptosis. In MIRI rats, borneol pretreatment significantly reduced serum levels of CK-MB, LDH and cTnI, decreased myocardial infarction area, and improved myocardial injury in different degree. Western blot results showed that borneol pretreatment significantly reduced the expression of Bcl-2-associated X protein (Bax) and Cysteine-aspartate protease-3 (Caspase-3) in cells and myocardial tissues of rats. Conclusion: Borneol can protect myocardial injury cells and mitigate MIRI by inhibiting cardiomyocyte apoptosis. |
| format | Article |
| id | doaj-art-fb12aec5588a42dbad35895df30bb024 |
| institution | OA Journals |
| issn | 1678-2674 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj-art-fb12aec5588a42dbad35895df30bb0242025-08-20T01:54:45ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742025-03-014010.1590/acb402225Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury ratsHui Zhanghttps://orcid.org/0009-0003-3876-6546Junfang Donghttps://orcid.org/0009-0006-9106-0851Jianwu Zhanghttps://orcid.org/0000-0002-9689-5448Hongxue Chenhttps://orcid.org/0009-0004-4613-225XTing Liuhttps://orcid.org/0009-0008-7221-6923Ruixue Ganhttps://orcid.org/0009-0008-3999-8564Jing Wenhttps://orcid.org/0000-0001-6980-9105Yangyou Lihttps://orcid.org/0000-0002-3574-7986ABSTRACT Purpose: To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis. Methods: Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by biochemical assay kit. Detection of apoptosis was by flow cytometry. Serum levels of creatine kinase isoenzyme MB (CK-MB), LDH, and cardiac troponin I (cTnI) were detected by enzyme-linked immunosorbent assay. Myocardial infarction area and pathological changes were observed via 2,3,5-triphenyltetrazolium chloride (TTC) staining and hematoxylin and eosin staining. The expressions of apoptosis-related proteins in cells and myocardial tissues were detected by Western blot. Results: H9c2 cell viability was significantly increased by pretreatment with 16 and 32 μg/mL of borneol. Borneol pretreatment significantly increased the T-SOD levels and reduced LDH leakage and apoptosis. In MIRI rats, borneol pretreatment significantly reduced serum levels of CK-MB, LDH and cTnI, decreased myocardial infarction area, and improved myocardial injury in different degree. Western blot results showed that borneol pretreatment significantly reduced the expression of Bcl-2-associated X protein (Bax) and Cysteine-aspartate protease-3 (Caspase-3) in cells and myocardial tissues of rats. Conclusion: Borneol can protect myocardial injury cells and mitigate MIRI by inhibiting cardiomyocyte apoptosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100209&tlng=enReperfusion InjuryMyocardial InfarctionApoptosis |
| spellingShingle | Hui Zhang Junfang Dong Jianwu Zhang Hongxue Chen Ting Liu Ruixue Gan Jing Wen Yangyou Li Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats Acta Cirúrgica Brasileira Reperfusion Injury Myocardial Infarction Apoptosis |
| title | Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats |
| title_full | Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats |
| title_fullStr | Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats |
| title_full_unstemmed | Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats |
| title_short | Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats |
| title_sort | effects of borneol on apoptosis of hypoxia reoxygenation h9c2 cells and myocardial ischemia reperfusion injury rats |
| topic | Reperfusion Injury Myocardial Infarction Apoptosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100209&tlng=en |
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