Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott
<b>Background:</b> This is a comparative metabolomic study of the medicinal plant <i>Dryopteris fragrans</i> (L.) Schott from the family <i>Dryopteridaceae</i> Herter (or <i>Aspidiaceae</i> Mett. ex Frank) growing under cold pole conditions in the Oymy...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/18/3/299 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850090884923654144 |
|---|---|
| author | Mayya P. Razgonova Zhanna M. Okhlopkova Muhammad A. Nawaz Polina S. Egorova Kirill S. Golokhvast |
| author_facet | Mayya P. Razgonova Zhanna M. Okhlopkova Muhammad A. Nawaz Polina S. Egorova Kirill S. Golokhvast |
| author_sort | Mayya P. Razgonova |
| collection | DOAJ |
| description | <b>Background:</b> This is a comparative metabolomic study of the medicinal plant <i>Dryopteris fragrans</i> (L.) Schott from the family <i>Dryopteridaceae</i> Herter (or <i>Aspidiaceae</i> Mett. ex Frank) growing under cold pole conditions in the Oymyakon region of the Republic of Sakha (Yakutia). <b>Methods:</b> The aerial parts of <i>D. fragrans</i> were subjected to extraction using supercritical CO<sub>2</sub> extraction and maceration methods. Several experimental conditions were investigated, including a pressure range of 50–300 bar and a temperature range of 31–60 °C. A 1% volume of ethanol was used as a co-solvent in the liquid phase of the extraction. <b>Results:</b> The most effective <i>D. fragrans</i> extraction conditions were 200 Bar pressure and a temperature of 55 °C. Tandem mass spectrometry was used to detect the target analytes. A total of 141 bioactive compounds (86 compounds from the polyphenol group and 55 compounds from other chemical groups) were tentatively identified in extracts of aerial parts of <i>D. fragrans</i>. Among these, thirty chemical constituents from the polyphenol group were identified for the first time. Other compound classes that were newly identified in <i>D. fragrans</i> include naphthoquinones (5,8-dihydroxy-6-methyl-2,3-dihydro-1,4-naphthoquinone, 1,8-dihydroxy-anthraquinone, 1,4,8-trihydroxyanthraquinone, chrysophanol, etc.), diterpenoids (tanshinone IIa, cryptotanshinone, isocryptotanshinone II, tanshinone IIb, etc.), polysaccharides, triterpenoids, and sesquiterpenes. <b>Conclusions:</b> These results highlight that <i>D. fragrans</i> is rich in bioactive compounds and put forward several newly detected compounds for further investigation. |
| format | Article |
| id | doaj-art-fb0f054effb844e2b88d546fcc1ba825 |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-fb0f054effb844e2b88d546fcc1ba8252025-08-20T02:42:28ZengMDPI AGPharmaceuticals1424-82472025-02-0118329910.3390/ph18030299Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) SchottMayya P. Razgonova0Zhanna M. Okhlopkova1Muhammad A. Nawaz2Polina S. Egorova3Kirill S. Golokhvast4N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B., Saint-Petersburg 190000, RussiaDepartment of Biology, North-Eastern Federal University, Yakutsk 677000, RussiaAdvanced Engineering School «Agrobiotek», National Research Tomsk State University, Tomsk 634050, RussiaYakutsk Botanical Garden, Institute for Biological Problems of Cryolithozone, Siberian Branch, Russian Academy Sciences, Yakutsk 677007, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, B., Saint-Petersburg 190000, Russia<b>Background:</b> This is a comparative metabolomic study of the medicinal plant <i>Dryopteris fragrans</i> (L.) Schott from the family <i>Dryopteridaceae</i> Herter (or <i>Aspidiaceae</i> Mett. ex Frank) growing under cold pole conditions in the Oymyakon region of the Republic of Sakha (Yakutia). <b>Methods:</b> The aerial parts of <i>D. fragrans</i> were subjected to extraction using supercritical CO<sub>2</sub> extraction and maceration methods. Several experimental conditions were investigated, including a pressure range of 50–300 bar and a temperature range of 31–60 °C. A 1% volume of ethanol was used as a co-solvent in the liquid phase of the extraction. <b>Results:</b> The most effective <i>D. fragrans</i> extraction conditions were 200 Bar pressure and a temperature of 55 °C. Tandem mass spectrometry was used to detect the target analytes. A total of 141 bioactive compounds (86 compounds from the polyphenol group and 55 compounds from other chemical groups) were tentatively identified in extracts of aerial parts of <i>D. fragrans</i>. Among these, thirty chemical constituents from the polyphenol group were identified for the first time. Other compound classes that were newly identified in <i>D. fragrans</i> include naphthoquinones (5,8-dihydroxy-6-methyl-2,3-dihydro-1,4-naphthoquinone, 1,8-dihydroxy-anthraquinone, 1,4,8-trihydroxyanthraquinone, chrysophanol, etc.), diterpenoids (tanshinone IIa, cryptotanshinone, isocryptotanshinone II, tanshinone IIb, etc.), polysaccharides, triterpenoids, and sesquiterpenes. <b>Conclusions:</b> These results highlight that <i>D. fragrans</i> is rich in bioactive compounds and put forward several newly detected compounds for further investigation.https://www.mdpi.com/1424-8247/18/3/299<i>Dryopteris fragrans</i> (L.)tandem mass spectrometrysupercritical CO<sub>2</sub> extractionpolyphenolsmetabolome |
| spellingShingle | Mayya P. Razgonova Zhanna M. Okhlopkova Muhammad A. Nawaz Polina S. Egorova Kirill S. Golokhvast Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott Pharmaceuticals <i>Dryopteris fragrans</i> (L.) tandem mass spectrometry supercritical CO<sub>2</sub> extraction polyphenols metabolome |
| title | Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott |
| title_full | Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott |
| title_fullStr | Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott |
| title_full_unstemmed | Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott |
| title_short | Supercritical Extraction and Identification of Bioactive Compounds in <i>Dryopteris fragrans</i> (L.) Schott |
| title_sort | supercritical extraction and identification of bioactive compounds in i dryopteris fragrans i l schott |
| topic | <i>Dryopteris fragrans</i> (L.) tandem mass spectrometry supercritical CO<sub>2</sub> extraction polyphenols metabolome |
| url | https://www.mdpi.com/1424-8247/18/3/299 |
| work_keys_str_mv | AT mayyaprazgonova supercriticalextractionandidentificationofbioactivecompoundsinidryopterisfragransilschott AT zhannamokhlopkova supercriticalextractionandidentificationofbioactivecompoundsinidryopterisfragransilschott AT muhammadanawaz supercriticalextractionandidentificationofbioactivecompoundsinidryopterisfragransilschott AT polinasegorova supercriticalextractionandidentificationofbioactivecompoundsinidryopterisfragransilschott AT kirillsgolokhvast supercriticalextractionandidentificationofbioactivecompoundsinidryopterisfragransilschott |