Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study
Background: Alpha-amylase is an enzyme found in saliva that plays a crucial role in the initial stages of digestion. Its levels can be influenced by various factors, including lifestyle habits such as smoking. Materials and Methods: A total of 150 male subjects were recruited for this comparative st...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_690_24 |
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| author | Amit A. Basannavar Ankita Bhargava Alkananda Sahoo Nidhi Dhakar Rahul R. Shetty Debanti Giri |
| author_facet | Amit A. Basannavar Ankita Bhargava Alkananda Sahoo Nidhi Dhakar Rahul R. Shetty Debanti Giri |
| author_sort | Amit A. Basannavar |
| collection | DOAJ |
| description | Background:
Alpha-amylase is an enzyme found in saliva that plays a crucial role in the initial stages of digestion. Its levels can be influenced by various factors, including lifestyle habits such as smoking.
Materials and Methods:
A total of 150 male subjects were recruited for this comparative study and divided into three groups: 50 smokers without any oral lesions (group A), 50 smokers with leukoplakia (group B), and 50 smokers with oral cancer (group C). Salivary samples were collected from each participant and analyzed for alpha-amylase activity using the 2-chloro-4-nitrophenyl-β-D-maltotrioside (CNPG3) method. Data were statistically analyzed using ANOVA and post-hoc tests to compare the mean alpha-amylase levels between the groups.
Results:
The mean alpha-amylase levels were found to be 140 U/mL in group A, 180 U/mL in group B, and 220 U/mL in group C. Statistical analysis revealed a significant increase in alpha-amylase levels in smokers with leukoplakia and oral cancer compared to smokers without oral lesions (P < 0.05). Moreover, a significant difference was observed between group B and group C (P < 0.05), indicating progressively higher enzyme levels with the severity of the oral condition.
Conclusion:
The study demonstrates that salivary alpha-amylase levels are elevated in smokers with leukoplakia and oral cancer compared to those without these conditions. |
| format | Article |
| id | doaj-art-fb00c929958b42f5b49e3fa9514c980f |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-fb00c929958b42f5b49e3fa9514c980f2025-01-12T14:10:56ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S3200S320210.4103/jpbs.jpbs_690_24Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative StudyAmit A. BasannavarAnkita BhargavaAlkananda SahooNidhi DhakarRahul R. ShettyDebanti GiriBackground: Alpha-amylase is an enzyme found in saliva that plays a crucial role in the initial stages of digestion. Its levels can be influenced by various factors, including lifestyle habits such as smoking. Materials and Methods: A total of 150 male subjects were recruited for this comparative study and divided into three groups: 50 smokers without any oral lesions (group A), 50 smokers with leukoplakia (group B), and 50 smokers with oral cancer (group C). Salivary samples were collected from each participant and analyzed for alpha-amylase activity using the 2-chloro-4-nitrophenyl-β-D-maltotrioside (CNPG3) method. Data were statistically analyzed using ANOVA and post-hoc tests to compare the mean alpha-amylase levels between the groups. Results: The mean alpha-amylase levels were found to be 140 U/mL in group A, 180 U/mL in group B, and 220 U/mL in group C. Statistical analysis revealed a significant increase in alpha-amylase levels in smokers with leukoplakia and oral cancer compared to smokers without oral lesions (P < 0.05). Moreover, a significant difference was observed between group B and group C (P < 0.05), indicating progressively higher enzyme levels with the severity of the oral condition. Conclusion: The study demonstrates that salivary alpha-amylase levels are elevated in smokers with leukoplakia and oral cancer compared to those without these conditions.https://journals.lww.com/10.4103/jpbs.jpbs_690_24alpha-amylasebiomarkercomparative studyleukoplakiaoral cancersalivasmokers |
| spellingShingle | Amit A. Basannavar Ankita Bhargava Alkananda Sahoo Nidhi Dhakar Rahul R. Shetty Debanti Giri Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study Journal of Pharmacy and Bioallied Sciences alpha-amylase biomarker comparative study leukoplakia oral cancer saliva smokers |
| title | Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study |
| title_full | Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study |
| title_fullStr | Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study |
| title_full_unstemmed | Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study |
| title_short | Estimation of Alpha-Amylase in Smokers with and without Leukoplakia and Oral Cancer—A Comparative Study |
| title_sort | estimation of alpha amylase in smokers with and without leukoplakia and oral cancer a comparative study |
| topic | alpha-amylase biomarker comparative study leukoplakia oral cancer saliva smokers |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_690_24 |
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