A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context
Summary: Background: Crimean-Congo hemorrhagic fever is a tick-borne febrile illness with wide geographic distribution. In recent years the geographic range of CCHFV and its tick vector have increased, placing an increasing number of people at risk of CCHFV infection. Currently there are no widely...
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Elsevier
2025-05-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396425001422 |
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| author | David W. Hawman Shanna Leventhal Kimberly Meade-White William Graham Karen Gaffney Amit Khandhar Justin Murray Jessy Prado-Smith Carl Shaia Greg Saturday Henry Buda Lenny Moise Jesse Erasmus Heinz Feldmann |
| author_facet | David W. Hawman Shanna Leventhal Kimberly Meade-White William Graham Karen Gaffney Amit Khandhar Justin Murray Jessy Prado-Smith Carl Shaia Greg Saturday Henry Buda Lenny Moise Jesse Erasmus Heinz Feldmann |
| author_sort | David W. Hawman |
| collection | DOAJ |
| description | Summary: Background: Crimean-Congo hemorrhagic fever is a tick-borne febrile illness with wide geographic distribution. In recent years the geographic range of CCHFV and its tick vector have increased, placing an increasing number of people at risk of CCHFV infection. Currently there are no widely available vaccines and although the World Health Organization recommends ribavirin for treatment, its efficacy is unclear. Vaccines are critically needed for CCHFV. Methods: Here we evaluated a promising replicating RNA vaccine for CCHFV in a Cynomolgus macaque model of disease. Findings: In primed and boosted macaques, we found that our replicating RNA vaccine expressing the CCHFV nucleoprotein (repNP) was highly immunogenic, eliciting a robust non-neutralizing antibody response that conferred significant protection against CCHFV challenge. Macaques receiving a single repNP vaccination were partially protected against CCHFV challenge. Interpretation: Our data demonstrate that our repNP vaccine and NP-specific antibody can protect against CCHFV in non-human primates. Funding: This study was supported by the Intramural Research Program of the NIAID/NIH and the Medical CBRN Defense Consortium grant #MCDC2204-011. |
| format | Article |
| id | doaj-art-faefe9ba0eb648d186f86c3c02003e37 |
| institution | OA Journals |
| issn | 2352-3964 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj-art-faefe9ba0eb648d186f86c3c02003e372025-08-20T02:11:55ZengElsevierEBioMedicine2352-39642025-05-0111510569810.1016/j.ebiom.2025.105698A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in contextDavid W. Hawman0Shanna Leventhal1Kimberly Meade-White2William Graham3Karen Gaffney4Amit Khandhar5Justin Murray6Jessy Prado-Smith7Carl Shaia8Greg Saturday9Henry Buda10Lenny Moise11Jesse Erasmus12Heinz Feldmann13Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USA; HDT Bio, Seattle, WA, 98109, USA; Corresponding author. HDT Bio, Seattle, WA, 98109, USA.Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USASeromyx Systems, Woburn, MA, 01801, USAHDT Bio, Seattle, WA, 98109, USAHDT Bio, Seattle, WA, 98109, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USARocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USARocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USARocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USASeromyx Systems, Woburn, MA, 01801, USASeromyx Systems, Woburn, MA, 01801, USAHDT Bio, Seattle, WA, 98109, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, 59840, USA; Corresponding author.Summary: Background: Crimean-Congo hemorrhagic fever is a tick-borne febrile illness with wide geographic distribution. In recent years the geographic range of CCHFV and its tick vector have increased, placing an increasing number of people at risk of CCHFV infection. Currently there are no widely available vaccines and although the World Health Organization recommends ribavirin for treatment, its efficacy is unclear. Vaccines are critically needed for CCHFV. Methods: Here we evaluated a promising replicating RNA vaccine for CCHFV in a Cynomolgus macaque model of disease. Findings: In primed and boosted macaques, we found that our replicating RNA vaccine expressing the CCHFV nucleoprotein (repNP) was highly immunogenic, eliciting a robust non-neutralizing antibody response that conferred significant protection against CCHFV challenge. Macaques receiving a single repNP vaccination were partially protected against CCHFV challenge. Interpretation: Our data demonstrate that our repNP vaccine and NP-specific antibody can protect against CCHFV in non-human primates. Funding: This study was supported by the Intramural Research Program of the NIAID/NIH and the Medical CBRN Defense Consortium grant #MCDC2204-011.http://www.sciencedirect.com/science/article/pii/S2352396425001422Crimean-CongoAntibodyVaccineRNA vaccineNon-human primates |
| spellingShingle | David W. Hawman Shanna Leventhal Kimberly Meade-White William Graham Karen Gaffney Amit Khandhar Justin Murray Jessy Prado-Smith Carl Shaia Greg Saturday Henry Buda Lenny Moise Jesse Erasmus Heinz Feldmann A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context EBioMedicine Crimean-Congo Antibody Vaccine RNA vaccine Non-human primates |
| title | A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context |
| title_full | A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context |
| title_fullStr | A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context |
| title_full_unstemmed | A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context |
| title_short | A replicating RNA vaccine confers protection against Crimean-Congo hemorrhagic fever in cynomolgus macaquesResearch in context |
| title_sort | replicating rna vaccine confers protection against crimean congo hemorrhagic fever in cynomolgus macaquesresearch in context |
| topic | Crimean-Congo Antibody Vaccine RNA vaccine Non-human primates |
| url | http://www.sciencedirect.com/science/article/pii/S2352396425001422 |
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