Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion
Objective: To evaluate the expression of matrix metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) in pituitary tumors and investigate their correlation with circulating plasma proteins and cavernous sinus invasion. In addition, the Ki-67 index was also assessed....
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Bioscientifica
2025-01-01
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| Series: | Endocrine Oncology |
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| Online Access: | https://eo.bioscientifica.com/view/journals/eo/5/1/EO-24-0037.xml |
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| author | Ane Caroline Thé B Freire Raquel S Jallad Rafael L Batista Andrea Glezer Marcio C Machado Gilberto Ochman Valter A Cescato Malebranche Berardo Carneiro Cunha Neto Fernando P Frassetto Raphael S S de Medeiros Ericka B Trarbach |
| author_facet | Ane Caroline Thé B Freire Raquel S Jallad Rafael L Batista Andrea Glezer Marcio C Machado Gilberto Ochman Valter A Cescato Malebranche Berardo Carneiro Cunha Neto Fernando P Frassetto Raphael S S de Medeiros Ericka B Trarbach |
| author_sort | Ane Caroline Thé B Freire |
| collection | DOAJ |
| description | Objective: To evaluate the expression of matrix metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) in pituitary tumors and investigate their correlation with circulating plasma proteins and cavernous sinus invasion. In addition, the Ki-67 index was also assessed. Methods: Seventy-four patients (37 females) with pituitary adenomas were included, with preoperative peripheral blood collected in 29 cases. Tumor samples were evaluated for MMP-2, MMP-9, TIMP-2 and Ki-67 expression by immunohistochemistry. Protein plasma was semi-quantitatively detected using a commercial membrane antibody array. Results: Sixteen patients presented tumors invading the cavernous sinus. MMP-2 and TIMP-2 were slightly increased in these tumors compared to the noninvasive group, but the difference was not statistically significant. MMP-9 and TIMP-2 plasma concentrations did not correlate with tumor protein expression and also did not differ between the two groups. MMP-2 was not detected in plasma in any case. No statistically significant difference was observed when different tumor subtypes were considered. A significant difference was observed in tumor size (3.4 cm (2.8–4.9) vs 1.9 cm (1.3–2.6); P < 0.001) and in the Ki-67 index (1.8% (0.3–2.5) vs 0.5% (0.2–1.0); P = 0.01) between the invasive and noninvasive groups. Conclusions: In this cohort, we found no significant correlation between tissue and plasma levels of MMP-2, MMP-9 and TIMP-2 and cavernous sinus invasion in pituitary tumors. Further investigation is needed to elucidate the potential role of these markers in the invasiveness of pituitary tumors. |
| format | Article |
| id | doaj-art-fae7a72566df4200b34d75310f1a6518 |
| institution | Kabale University |
| issn | 2634-4793 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Oncology |
| spelling | doaj-art-fae7a72566df4200b34d75310f1a65182025-01-07T17:17:35ZengBioscientificaEndocrine Oncology2634-47932025-01-015110.1530/EO-24-00371Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasionAne Caroline Thé B Freire0Raquel S Jallad1Rafael L Batista2Andrea Glezer3Marcio C Machado4Gilberto Ochman5Valter A Cescato6Malebranche Berardo Carneiro Cunha Neto7Fernando P Frassetto8Raphael S S de Medeiros9Ericka B Trarbach10Unidade de Neuroendocrinologia, Disciplina de Endocrinologia e Metabologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilUnidade de Neuroendocrinologia, Disciplina de Endocrinologia e Metabologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilUnidade de Neuroendocrinologia, Disciplina de Endocrinologia e Metabologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilUnidade de Neuroendocrinologia, Disciplina de Endocrinologia e Metabologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilUnidade de Neuroendocrinologia, Disciplina de Endocrinologia e Metabologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilDivisao de Neurocirurgia Funcional, Instituto de Psiquiatria – IPq, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilDivisao de Neurocirurgia Funcional, Instituto de Psiquiatria – IPq, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilDivisao de Neurocirurgia Funcional, Instituto de Psiquiatria – IPq, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilDivisao de Anatomia Patológica, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilDivisao de Anatomia Patológica, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilLaboratorio de Endocrinologia Celular e Molecular, LIM25, Disciplina de Endocrinologia e Metabologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BrazilObjective: To evaluate the expression of matrix metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) in pituitary tumors and investigate their correlation with circulating plasma proteins and cavernous sinus invasion. In addition, the Ki-67 index was also assessed. Methods: Seventy-four patients (37 females) with pituitary adenomas were included, with preoperative peripheral blood collected in 29 cases. Tumor samples were evaluated for MMP-2, MMP-9, TIMP-2 and Ki-67 expression by immunohistochemistry. Protein plasma was semi-quantitatively detected using a commercial membrane antibody array. Results: Sixteen patients presented tumors invading the cavernous sinus. MMP-2 and TIMP-2 were slightly increased in these tumors compared to the noninvasive group, but the difference was not statistically significant. MMP-9 and TIMP-2 plasma concentrations did not correlate with tumor protein expression and also did not differ between the two groups. MMP-2 was not detected in plasma in any case. No statistically significant difference was observed when different tumor subtypes were considered. A significant difference was observed in tumor size (3.4 cm (2.8–4.9) vs 1.9 cm (1.3–2.6); P < 0.001) and in the Ki-67 index (1.8% (0.3–2.5) vs 0.5% (0.2–1.0); P = 0.01) between the invasive and noninvasive groups. Conclusions: In this cohort, we found no significant correlation between tissue and plasma levels of MMP-2, MMP-9 and TIMP-2 and cavernous sinus invasion in pituitary tumors. Further investigation is needed to elucidate the potential role of these markers in the invasiveness of pituitary tumors.https://eo.bioscientifica.com/view/journals/eo/5/1/EO-24-0037.xmlpituitary tumorsinvasivenessmatrix metalloproteinase-2matrix metalloproteinase-9tissue inhibitor of metalloproteinase-2 |
| spellingShingle | Ane Caroline Thé B Freire Raquel S Jallad Rafael L Batista Andrea Glezer Marcio C Machado Gilberto Ochman Valter A Cescato Malebranche Berardo Carneiro Cunha Neto Fernando P Frassetto Raphael S S de Medeiros Ericka B Trarbach Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion Endocrine Oncology pituitary tumors invasiveness matrix metalloproteinase-2 matrix metalloproteinase-9 tissue inhibitor of metalloproteinase-2 |
| title | Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion |
| title_full | Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion |
| title_fullStr | Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion |
| title_full_unstemmed | Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion |
| title_short | Expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and their relationship with cavernous sinus invasion |
| title_sort | expression of mmp 2 mmp 9 and timp 2 in pituitary tumors and their relationship with cavernous sinus invasion |
| topic | pituitary tumors invasiveness matrix metalloproteinase-2 matrix metalloproteinase-9 tissue inhibitor of metalloproteinase-2 |
| url | https://eo.bioscientifica.com/view/journals/eo/5/1/EO-24-0037.xml |
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