MicroRNA Profiling: From Dark Matter to White Matter, or Identifying New Players in Neurobiology

Contemporary biology has been revolutionized by a recently discovered class of small regulatory RNA molecules, microRNAs (miRNAs). Missed by researchers for decades due to their tiny size, usually mapping to non-protein-coding regions of genomes, miRNAs and miRNA-mediated regulatory networks have be...

Full description

Saved in:
Bibliographic Details
Main Author: Anna M. Krichevsky
Format: Article
Language:English
Published: Wiley 2007-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2007.201
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Contemporary biology has been revolutionized by a recently discovered class of small regulatory RNA molecules, microRNAs (miRNAs). Missed by researchers for decades due to their tiny size, usually mapping to non-protein-coding regions of genomes, miRNAs and miRNA-mediated regulatory networks have been the “dark matter” of molecular biology. Deciphering miRNA pathways and functions in the CNS of complex organisms is tightly linked to understanding miRNA expression patterns. To facilitate these emerging studies, I here review the basic principles of medium- and high-throughput technologies available for miRNA expression profiling.
ISSN:1537-744X