Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis
Peroxiredoxin6 (Prdx6) is a bifunctional antioxidant enzyme with both peroxidase and phospholipase A₂ activities. Although its molecular roles are well established, the developmental role of Prdx6 remains poorly understood. To address this gap in the literature, this study aimed to examine the in vi...
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2025.2533823 |
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| author | Minjoo Kim Hyun-Kyung Lee Hongchan Lee Hyun-Shik Lee |
| author_facet | Minjoo Kim Hyun-Kyung Lee Hongchan Lee Hyun-Shik Lee |
| author_sort | Minjoo Kim |
| collection | DOAJ |
| description | Peroxiredoxin6 (Prdx6) is a bifunctional antioxidant enzyme with both peroxidase and phospholipase A₂ activities. Although its molecular roles are well established, the developmental role of Prdx6 remains poorly understood. To address this gap in the literature, this study aimed to examine the in vivo function of Prdx6 in primitive myelopoiesis using Xenopus laevis embryos. We found that prdx6 is specifically expressed in myeloid progenitors originating from the anterior ventral blood island during early embryogenesis. Knockdown of prdx6 significantly reduced the number of myeloid cells, without affecting their migration ability. Embryos depleted of prdx6 exhibited elevated levels of reactive oxygen species (ROS) and decreased cellular proliferation. Co-injection of morpholino (MO)-resistant prdx6 mRNA or treatment with N-acetylcysteine (NAC) successfully restored both ROS levels and myeloid cell numbers, suggesting that Prdx6 supports primitive myeloid cell development by maintaining redox homeostasis. These findings reveal a novel role of Prdx6 in ROS-dependent proliferation of myeloid progenitors during early vertebrate development. |
| format | Article |
| id | doaj-art-fac97e07031d4c92a0dc7db8cef609cd |
| institution | Kabale University |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj-art-fac97e07031d4c92a0dc7db8cef609cd2025-08-20T03:50:58ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852025-12-0129143844510.1080/19768354.2025.2533823Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesisMinjoo Kim0Hyun-Kyung Lee1Hongchan Lee2Hyun-Shik Lee3KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Biotechnology, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daegu, Republic of KoreaKNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Biotechnology, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daegu, Republic of KoreaKNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Biotechnology, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daegu, Republic of KoreaKNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Biotechnology, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daegu, Republic of KoreaPeroxiredoxin6 (Prdx6) is a bifunctional antioxidant enzyme with both peroxidase and phospholipase A₂ activities. Although its molecular roles are well established, the developmental role of Prdx6 remains poorly understood. To address this gap in the literature, this study aimed to examine the in vivo function of Prdx6 in primitive myelopoiesis using Xenopus laevis embryos. We found that prdx6 is specifically expressed in myeloid progenitors originating from the anterior ventral blood island during early embryogenesis. Knockdown of prdx6 significantly reduced the number of myeloid cells, without affecting their migration ability. Embryos depleted of prdx6 exhibited elevated levels of reactive oxygen species (ROS) and decreased cellular proliferation. Co-injection of morpholino (MO)-resistant prdx6 mRNA or treatment with N-acetylcysteine (NAC) successfully restored both ROS levels and myeloid cell numbers, suggesting that Prdx6 supports primitive myeloid cell development by maintaining redox homeostasis. These findings reveal a novel role of Prdx6 in ROS-dependent proliferation of myeloid progenitors during early vertebrate development.https://www.tandfonline.com/doi/10.1080/19768354.2025.2533823Peroxiredoxin 6myeloid cellreactive oxygen speciesredox regulationXenopus laevis |
| spellingShingle | Minjoo Kim Hyun-Kyung Lee Hongchan Lee Hyun-Shik Lee Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis Animal Cells and Systems Peroxiredoxin 6 myeloid cell reactive oxygen species redox regulation Xenopus laevis |
| title | Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis |
| title_full | Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis |
| title_fullStr | Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis |
| title_full_unstemmed | Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis |
| title_short | Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis |
| title_sort | prdx6 regulates in vivo myeloid cell development via redox control during xenopus embryogenesis |
| topic | Peroxiredoxin 6 myeloid cell reactive oxygen species redox regulation Xenopus laevis |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2025.2533823 |
| work_keys_str_mv | AT minjookim prdx6regulatesinvivomyeloidcelldevelopmentviaredoxcontrolduringxenopusembryogenesis AT hyunkyunglee prdx6regulatesinvivomyeloidcelldevelopmentviaredoxcontrolduringxenopusembryogenesis AT hongchanlee prdx6regulatesinvivomyeloidcelldevelopmentviaredoxcontrolduringxenopusembryogenesis AT hyunshiklee prdx6regulatesinvivomyeloidcelldevelopmentviaredoxcontrolduringxenopusembryogenesis |