International network for comparison of HIV neutralization assays: the NeutNet report.
<h4>Background</h4>Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an internation...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004505&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850108054721265664 |
|---|---|
| author | Eva Maria Fenyö Alan Heath Stefania Dispinseri Harvey Holmes Paolo Lusso Susan Zolla-Pazner Helen Donners Leo Heyndrickx Jose Alcami Vera Bongertz Christian Jassoy Mauro Malnati David Montefiori Christiane Moog Lynn Morris Saladin Osmanov Victoria Polonis Quentin Sattentau Hanneke Schuitemaker Ruengpung Sutthent Terri Wrin Gabriella Scarlatti |
| author_facet | Eva Maria Fenyö Alan Heath Stefania Dispinseri Harvey Holmes Paolo Lusso Susan Zolla-Pazner Helen Donners Leo Heyndrickx Jose Alcami Vera Bongertz Christian Jassoy Mauro Malnati David Montefiori Christiane Moog Lynn Morris Saladin Osmanov Victoria Polonis Quentin Sattentau Hanneke Schuitemaker Ruengpung Sutthent Terri Wrin Gabriella Scarlatti |
| author_sort | Eva Maria Fenyö |
| collection | DOAJ |
| description | <h4>Background</h4>Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays.<h4>Methods</h4>Each laboratory evaluated four neutralizing reagents (TriMab, 447-52D, 4E10, sCD4) at a given range of concentrations against a panel of 11 viruses representing a wide range of genetic subtypes and phenotypes. A total of 16 different assays were compared. The assays utilized either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (virus infectivity assays, VI assays), or their Env-pseudotyped (gp160) derivatives produced in 293T cells (PSV assays) from molecular clones or uncloned virus. Target cells included PBMC and genetically-engineered cell lines in either a single- or multiple-cycle infection format. Infection was quantified by using a range of assay read-outs that included extracellular or intracellular p24 antigen detection, RNA quantification and luciferase and beta-galactosidase reporter gene expression.<h4>Findings</h4>PSV assays were generally more sensitive than VI assays, but there were important differences according to the virus and inhibitor used. For example, for TriMab, the mean IC50 was always lower in PSV than in VI assays. However, with 4E10 or sCD4 some viruses were neutralized with a lower IC50 in VI assays than in the PSV assays. Inter-laboratory concordance was slightly better for PSV than for VI assays with some viruses, but for other viruses agreement between laboratories was limited and depended on both the virus and the neutralizing reagent.<h4>Conclusions</h4>The NeutNet project demonstrated clear differences in assay sensitivity that were dependent on both the neutralizing reagent and the virus. No single assay was capable of detecting the entire spectrum of neutralizing activities. Since it is not known which in vitro assay correlates with in vivo protection, a range of neutralization assays is recommended for vaccine evaluation. |
| format | Article |
| id | doaj-art-fac6af32cdf44af3997336dd753f82d2 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-fac6af32cdf44af3997336dd753f82d22025-08-20T02:38:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0142e450510.1371/journal.pone.0004505International network for comparison of HIV neutralization assays: the NeutNet report.Eva Maria FenyöAlan HeathStefania DispinseriHarvey HolmesPaolo LussoSusan Zolla-PaznerHelen DonnersLeo HeyndrickxJose AlcamiVera BongertzChristian JassoyMauro MalnatiDavid MontefioriChristiane MoogLynn MorrisSaladin OsmanovVictoria PolonisQuentin SattentauHanneke SchuitemakerRuengpung SutthentTerri WrinGabriella Scarlatti<h4>Background</h4>Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays.<h4>Methods</h4>Each laboratory evaluated four neutralizing reagents (TriMab, 447-52D, 4E10, sCD4) at a given range of concentrations against a panel of 11 viruses representing a wide range of genetic subtypes and phenotypes. A total of 16 different assays were compared. The assays utilized either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (virus infectivity assays, VI assays), or their Env-pseudotyped (gp160) derivatives produced in 293T cells (PSV assays) from molecular clones or uncloned virus. Target cells included PBMC and genetically-engineered cell lines in either a single- or multiple-cycle infection format. Infection was quantified by using a range of assay read-outs that included extracellular or intracellular p24 antigen detection, RNA quantification and luciferase and beta-galactosidase reporter gene expression.<h4>Findings</h4>PSV assays were generally more sensitive than VI assays, but there were important differences according to the virus and inhibitor used. For example, for TriMab, the mean IC50 was always lower in PSV than in VI assays. However, with 4E10 or sCD4 some viruses were neutralized with a lower IC50 in VI assays than in the PSV assays. Inter-laboratory concordance was slightly better for PSV than for VI assays with some viruses, but for other viruses agreement between laboratories was limited and depended on both the virus and the neutralizing reagent.<h4>Conclusions</h4>The NeutNet project demonstrated clear differences in assay sensitivity that were dependent on both the neutralizing reagent and the virus. No single assay was capable of detecting the entire spectrum of neutralizing activities. Since it is not known which in vitro assay correlates with in vivo protection, a range of neutralization assays is recommended for vaccine evaluation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004505&type=printable |
| spellingShingle | Eva Maria Fenyö Alan Heath Stefania Dispinseri Harvey Holmes Paolo Lusso Susan Zolla-Pazner Helen Donners Leo Heyndrickx Jose Alcami Vera Bongertz Christian Jassoy Mauro Malnati David Montefiori Christiane Moog Lynn Morris Saladin Osmanov Victoria Polonis Quentin Sattentau Hanneke Schuitemaker Ruengpung Sutthent Terri Wrin Gabriella Scarlatti International network for comparison of HIV neutralization assays: the NeutNet report. PLoS ONE |
| title | International network for comparison of HIV neutralization assays: the NeutNet report. |
| title_full | International network for comparison of HIV neutralization assays: the NeutNet report. |
| title_fullStr | International network for comparison of HIV neutralization assays: the NeutNet report. |
| title_full_unstemmed | International network for comparison of HIV neutralization assays: the NeutNet report. |
| title_short | International network for comparison of HIV neutralization assays: the NeutNet report. |
| title_sort | international network for comparison of hiv neutralization assays the neutnet report |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004505&type=printable |
| work_keys_str_mv | AT evamariafenyo internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT alanheath internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT stefaniadispinseri internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT harveyholmes internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT paololusso internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT susanzollapazner internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT helendonners internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT leoheyndrickx internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT josealcami internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT verabongertz internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT christianjassoy internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT mauromalnati internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT davidmontefiori internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT christianemoog internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT lynnmorris internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT saladinosmanov internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT victoriapolonis internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT quentinsattentau internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT hannekeschuitemaker internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT ruengpungsutthent internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT terriwrin internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport AT gabriellascarlatti internationalnetworkforcomparisonofhivneutralizationassaystheneutnetreport |