Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital
Background: The challenge of treating infections caused by multidrug-resistant Enterobacterales and Acinetobacter baumannii has significantly increased for medical professionals due to their resistance to conventional antibiotics. In such cases, colistin is employed as a final line of defense. This...
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Zagazig University, Faculty of Medicine
2024-08-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_355556_7d0654ef26ae31ae84d28cc32f812b26.pdf |
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| author | Nadia El mrimar El mehdi Belouad Elmostafa Benaissa Adil Maleb Mostafa Elouennass |
| author_facet | Nadia El mrimar El mehdi Belouad Elmostafa Benaissa Adil Maleb Mostafa Elouennass |
| author_sort | Nadia El mrimar |
| collection | DOAJ |
| description | Background: The challenge of treating infections caused by multidrug-resistant Enterobacterales and Acinetobacter baumannii has significantly increased for medical professionals due to their resistance to conventional antibiotics. In such cases, colistin is employed as a final line of defense. This study was aimed to determine the in-vitro efficacy of colistin against multidrug-resistant gram-negative bacteria. Methods: The research was carried out in the bacteriology department of the Mohammed V Military Teaching Hospital in Rabat, Morocco. 321 isolates of multi-resistant Enterobacterales and Acinetobacter baumannii from various clinical samples were identified by standard microbiological protocols, and the Colistin minimum inhibitory concentrations value was determined using the microdilution method. Results: Of the 321 isolates included in the study, 76.3% were Enterobacterales and 23.6% were Acinetobacter baumannii. The minimum inhibitory analysis showed that 96.3% of the isolates were sensitive, while 3.7% were identified as resistant. The prevalence of resistance to colistin among multi-resistant Enterobacterales was 4.1%, and the MIC50 and MIC90 were 0.5 μg/ml and 1μg/ml respectively. Among the collected Acinetobacter baumannii isolates, the prevalence of colistin resistance was 2.6%, with the MIC50 and MIC90 of 0.5 μg/ml. Conclusion: The research indicates that colistin could be a viable treatment option for infections caused by multi-resistant Enterobacterales and Acinetobacter baumannii. |
| format | Article |
| id | doaj-art-fac3afea189542789254fbab48dc9442 |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj-art-fac3afea189542789254fbab48dc94422024-12-21T21:03:40ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-08-01531139114610.21608/mid.2024.279217.1862355556Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospitalNadia El mrimar0El mehdi Belouad1Elmostafa Benaissa2Adil Maleb3Mostafa Elouennass4Research Team of Epidemiology and Bacterial Resistance, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, MoroccoResearch Team of Epidemiology and Bacterial Resistance, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, MoroccoResearch Team of Epidemiology and Bacterial Resistance, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, MoroccoLaboratory of Microbiology, Mohammed VI University Hospital, Faculty of Medicine and Pharmacy, University Mohammed the First, Oujda, MoroccoResearch Team of Epidemiology and Bacterial Resistance, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, MoroccoBackground: The challenge of treating infections caused by multidrug-resistant Enterobacterales and Acinetobacter baumannii has significantly increased for medical professionals due to their resistance to conventional antibiotics. In such cases, colistin is employed as a final line of defense. This study was aimed to determine the in-vitro efficacy of colistin against multidrug-resistant gram-negative bacteria. Methods: The research was carried out in the bacteriology department of the Mohammed V Military Teaching Hospital in Rabat, Morocco. 321 isolates of multi-resistant Enterobacterales and Acinetobacter baumannii from various clinical samples were identified by standard microbiological protocols, and the Colistin minimum inhibitory concentrations value was determined using the microdilution method. Results: Of the 321 isolates included in the study, 76.3% were Enterobacterales and 23.6% were Acinetobacter baumannii. The minimum inhibitory analysis showed that 96.3% of the isolates were sensitive, while 3.7% were identified as resistant. The prevalence of resistance to colistin among multi-resistant Enterobacterales was 4.1%, and the MIC50 and MIC90 were 0.5 μg/ml and 1μg/ml respectively. Among the collected Acinetobacter baumannii isolates, the prevalence of colistin resistance was 2.6%, with the MIC50 and MIC90 of 0.5 μg/ml. Conclusion: The research indicates that colistin could be a viable treatment option for infections caused by multi-resistant Enterobacterales and Acinetobacter baumannii.https://mid.journals.ekb.eg/article_355556_7d0654ef26ae31ae84d28cc32f812b26.pdfcolistinantibiotic resistancemultidrug-resistant enterobacteralesacinetobacter baumanniigram-negative bacteria |
| spellingShingle | Nadia El mrimar El mehdi Belouad Elmostafa Benaissa Adil Maleb Mostafa Elouennass Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital Microbes and Infectious Diseases colistin antibiotic resistance multidrug-resistant enterobacterales acinetobacter baumannii gram-negative bacteria |
| title | Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital |
| title_full | Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital |
| title_fullStr | Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital |
| title_full_unstemmed | Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital |
| title_short | Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital |
| title_sort | antimicrobial resistance of multidrug resistant enterobacterales and acinetobacter baumannii isolates to colistin in a moroccan hospital |
| topic | colistin antibiotic resistance multidrug-resistant enterobacterales acinetobacter baumannii gram-negative bacteria |
| url | https://mid.journals.ekb.eg/article_355556_7d0654ef26ae31ae84d28cc32f812b26.pdf |
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