Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions
Summary: Here, we present a protocol for differentiating 3T3-L1 preadipocytes and stromal vascular fraction (SVF)-derived preadipocytes from mice into mature adipocytes, followed by the isolation of crude mitochondrial fractions. This cost-effective and reproducible protocol is optimized for small-p...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004514 |
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| author | Churaibhon Wisessaowapak Jeongmin Lee Hyeonhui Kim Seunghwan Son Xue Feng Lina Chang Annie Hoang Hetty Chen Sarah Bedsted Alan R. Saltiel |
| author_facet | Churaibhon Wisessaowapak Jeongmin Lee Hyeonhui Kim Seunghwan Son Xue Feng Lina Chang Annie Hoang Hetty Chen Sarah Bedsted Alan R. Saltiel |
| author_sort | Churaibhon Wisessaowapak |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for differentiating 3T3-L1 preadipocytes and stromal vascular fraction (SVF)-derived preadipocytes from mice into mature adipocytes, followed by the isolation of crude mitochondrial fractions. This cost-effective and reproducible protocol is optimized for small-plate formats, compatible with standard reagents, and suitable for metabolic studies such as insulin resistance and mitochondrial function. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-faaec5f58d5e46fbbd3cafd55cedf191 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-faaec5f58d5e46fbbd3cafd55cedf1912025-08-25T04:14:48ZengElsevierSTAR Protocols2666-16672025-09-016310404510.1016/j.xpro.2025.104045Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractionsChuraibhon Wisessaowapak0Jeongmin Lee1Hyeonhui Kim2Seunghwan Son3Xue Feng4Lina Chang5Annie Hoang6Hetty Chen7Sarah Bedsted8Alan R. Saltiel9Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USA; Corresponding authorDivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USADivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USA; Department of Pharmacology, University of California, San Diego, San Diego, CA, USA; Corresponding authorSummary: Here, we present a protocol for differentiating 3T3-L1 preadipocytes and stromal vascular fraction (SVF)-derived preadipocytes from mice into mature adipocytes, followed by the isolation of crude mitochondrial fractions. This cost-effective and reproducible protocol is optimized for small-plate formats, compatible with standard reagents, and suitable for metabolic studies such as insulin resistance and mitochondrial function. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725004514Cell cultureMetabolismMolecular Biology |
| spellingShingle | Churaibhon Wisessaowapak Jeongmin Lee Hyeonhui Kim Seunghwan Son Xue Feng Lina Chang Annie Hoang Hetty Chen Sarah Bedsted Alan R. Saltiel Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions STAR Protocols Cell culture Metabolism Molecular Biology |
| title | Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions |
| title_full | Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions |
| title_fullStr | Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions |
| title_full_unstemmed | Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions |
| title_short | Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions |
| title_sort | protocol for differentiating murine 3t3 l1 and svf derived preadipocytes and isolating crude mitochondrial fractions |
| topic | Cell culture Metabolism Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725004514 |
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