Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation
In Mediterranean countries, Sarcophaga (Liopygia) crassipalpis, Sarcophaga (L.) argyrostoma, and Sarcophaga (L.) cultellata share the same ecological niche and can be responsible of myiasis. In this study, the main morphological characters of a larva found in a hospitalized woman were described and...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2017/9064531 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554468889067520 |
|---|---|
| author | Annunziata Giangaspero Marianna Marangi Antonio Balotta Claudio Venturelli Krzysztof Szpila Antonella Di Palma |
| author_facet | Annunziata Giangaspero Marianna Marangi Antonio Balotta Claudio Venturelli Krzysztof Szpila Antonella Di Palma |
| author_sort | Annunziata Giangaspero |
| collection | DOAJ |
| description | In Mediterranean countries, Sarcophaga (Liopygia) crassipalpis, Sarcophaga (L.) argyrostoma, and Sarcophaga (L.) cultellata share the same ecological niche and can be responsible of myiasis. In this study, the main morphological characters of a larva found in a hospitalized woman were described and illustrated by light and SEM microscopy and the features discussed. Then, a fragment within the mitochondrial encoded cytochrome c oxidase subunit I (coxI) gene of ~735 bp was amplified and sequenced. The molecular investigation was necessary to confirm the species Sarcophaga (Liopygia) argyrostoma (99% of identity). Our findings showed that morphological descriptions of larvae of three Mediterranean species of Liopygia available in several papers might not be clear enough to allow for comparison and correct identification. Until results of reliable comparative studies of larvae of all three species will be available, the use of molecular tools is crucial, to avoid misleading or incomplete identification, and in particular when a myiasis becomes a legal issue. |
| format | Article |
| id | doaj-art-fa8f6424a28c434a9c7efc45015711e2 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-fa8f6424a28c434a9c7efc45015711e22025-02-03T05:51:34ZengWileyThe Scientific World Journal2356-61401537-744X2017-01-01201710.1155/2017/90645319064531Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular InvestigationAnnunziata Giangaspero0Marianna Marangi1Antonio Balotta2Claudio Venturelli3Krzysztof Szpila4Antonella Di Palma5Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, ItalyDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, ItalyUnità Operativa di Geriatria, Ospedale M. Bufalini, AUSL della Romagna, Ravenna, ItalyDipartimento di Sanità Pubblica, AUSL della Romagna, Ravenna, ItalyChair of Ecology and Biogeography, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, ItalyIn Mediterranean countries, Sarcophaga (Liopygia) crassipalpis, Sarcophaga (L.) argyrostoma, and Sarcophaga (L.) cultellata share the same ecological niche and can be responsible of myiasis. In this study, the main morphological characters of a larva found in a hospitalized woman were described and illustrated by light and SEM microscopy and the features discussed. Then, a fragment within the mitochondrial encoded cytochrome c oxidase subunit I (coxI) gene of ~735 bp was amplified and sequenced. The molecular investigation was necessary to confirm the species Sarcophaga (Liopygia) argyrostoma (99% of identity). Our findings showed that morphological descriptions of larvae of three Mediterranean species of Liopygia available in several papers might not be clear enough to allow for comparison and correct identification. Until results of reliable comparative studies of larvae of all three species will be available, the use of molecular tools is crucial, to avoid misleading or incomplete identification, and in particular when a myiasis becomes a legal issue.http://dx.doi.org/10.1155/2017/9064531 |
| spellingShingle | Annunziata Giangaspero Marianna Marangi Antonio Balotta Claudio Venturelli Krzysztof Szpila Antonella Di Palma Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation The Scientific World Journal |
| title | Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation |
| title_full | Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation |
| title_fullStr | Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation |
| title_full_unstemmed | Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation |
| title_short | Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation |
| title_sort | wound myiasis caused by sarcophaga liopygia argyrostoma robineau desvoidy diptera sarcophagidae additional evidences of the morphological identification dilemma and molecular investigation |
| url | http://dx.doi.org/10.1155/2017/9064531 |
| work_keys_str_mv | AT annunziatagiangaspero woundmyiasiscausedbysarcophagaliopygiaargyrostomarobineaudesvoidydipterasarcophagidaeadditionalevidencesofthemorphologicalidentificationdilemmaandmolecularinvestigation AT mariannamarangi woundmyiasiscausedbysarcophagaliopygiaargyrostomarobineaudesvoidydipterasarcophagidaeadditionalevidencesofthemorphologicalidentificationdilemmaandmolecularinvestigation AT antoniobalotta woundmyiasiscausedbysarcophagaliopygiaargyrostomarobineaudesvoidydipterasarcophagidaeadditionalevidencesofthemorphologicalidentificationdilemmaandmolecularinvestigation AT claudioventurelli woundmyiasiscausedbysarcophagaliopygiaargyrostomarobineaudesvoidydipterasarcophagidaeadditionalevidencesofthemorphologicalidentificationdilemmaandmolecularinvestigation AT krzysztofszpila woundmyiasiscausedbysarcophagaliopygiaargyrostomarobineaudesvoidydipterasarcophagidaeadditionalevidencesofthemorphologicalidentificationdilemmaandmolecularinvestigation AT antonelladipalma woundmyiasiscausedbysarcophagaliopygiaargyrostomarobineaudesvoidydipterasarcophagidaeadditionalevidencesofthemorphologicalidentificationdilemmaandmolecularinvestigation |