Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway
Abstract As a strategic resource for both medicine and essential oil, the healthy development of the Pogostemon cablin industry is crucial for the traditional medicine and fragrance sectors. Bacterial wilt represents one of the most significant threats to patchouli cultivation; however, the molecula...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-07009-4 |
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| author | Jian Li Dan-Xia Wu He-Nan Bao Ke-Yu Li Ming-Yong Zhang Yun-Hao Sun Kuai-Fei Xia |
| author_facet | Jian Li Dan-Xia Wu He-Nan Bao Ke-Yu Li Ming-Yong Zhang Yun-Hao Sun Kuai-Fei Xia |
| author_sort | Jian Li |
| collection | DOAJ |
| description | Abstract As a strategic resource for both medicine and essential oil, the healthy development of the Pogostemon cablin industry is crucial for the traditional medicine and fragrance sectors. Bacterial wilt represents one of the most significant threats to patchouli cultivation; however, the molecular mechanisms underlying P. cablin’s response to bacterial wilt remain unexplored. Here, we conducted transcriptome and metabolome analyses, revealing an increase in the expression of genes associated with lipid pathways and a corresponding rise in the concentration of lipid metabolites in P. cablin following infection by the bacterial wilt pathogen SY1. Further lipidomics analysis demonstrated a significant upregulation of ceramide levels due to SY1 infection. Additionally, hormone analysis indicated that SY1 significantly induced an increase in abscisic acid (ABA) concentration, accompanied by the upregulation of genes involved in the ABA synthesis pathway and its downstream signaling pathways. Furthermore, we treated P. cablin seedlings with the ceramide synthase inhibitor FB1, which significantly reduced ceramide concentration in P. cablin. FB1 treatment also inhibited the expression of ABA-synthesizing genes, leading to a notable decrease in ABA concentration and downstream pathway genes. These data indicate that ceramides and ABA may participate in P. cablin’s response to SY1. |
| format | Article |
| id | doaj-art-fa855a9cdbf14e19a6e9abf93c2ab12a |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-fa855a9cdbf14e19a6e9abf93c2ab12a2025-08-20T03:45:59ZengBMCBMC Plant Biology1471-22292025-07-0125111310.1186/s12870-025-07009-4Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathwayJian Li0Dan-Xia Wu1He-Nan Bao2Ke-Yu Li3Ming-Yong Zhang4Yun-Hao Sun5Kuai-Fei Xia6Guangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of SciencesState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of SciencesCollege of Agriculture and Biology, Zhongkai University of Agriculture and EngineeringGuangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of SciencesAbstract As a strategic resource for both medicine and essential oil, the healthy development of the Pogostemon cablin industry is crucial for the traditional medicine and fragrance sectors. Bacterial wilt represents one of the most significant threats to patchouli cultivation; however, the molecular mechanisms underlying P. cablin’s response to bacterial wilt remain unexplored. Here, we conducted transcriptome and metabolome analyses, revealing an increase in the expression of genes associated with lipid pathways and a corresponding rise in the concentration of lipid metabolites in P. cablin following infection by the bacterial wilt pathogen SY1. Further lipidomics analysis demonstrated a significant upregulation of ceramide levels due to SY1 infection. Additionally, hormone analysis indicated that SY1 significantly induced an increase in abscisic acid (ABA) concentration, accompanied by the upregulation of genes involved in the ABA synthesis pathway and its downstream signaling pathways. Furthermore, we treated P. cablin seedlings with the ceramide synthase inhibitor FB1, which significantly reduced ceramide concentration in P. cablin. FB1 treatment also inhibited the expression of ABA-synthesizing genes, leading to a notable decrease in ABA concentration and downstream pathway genes. These data indicate that ceramides and ABA may participate in P. cablin’s response to SY1.https://doi.org/10.1186/s12870-025-07009-4Pogostemon cablinBacterial wiltPlant defense responseABA |
| spellingShingle | Jian Li Dan-Xia Wu He-Nan Bao Ke-Yu Li Ming-Yong Zhang Yun-Hao Sun Kuai-Fei Xia Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway BMC Plant Biology Pogostemon cablin Bacterial wilt Plant defense response ABA |
| title | Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway |
| title_full | Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway |
| title_fullStr | Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway |
| title_full_unstemmed | Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway |
| title_short | Ceramides play a significant role in the response of Pogostemon cablin to bacterial wilt by regulating the ABA pathway |
| title_sort | ceramides play a significant role in the response of pogostemon cablin to bacterial wilt by regulating the aba pathway |
| topic | Pogostemon cablin Bacterial wilt Plant defense response ABA |
| url | https://doi.org/10.1186/s12870-025-07009-4 |
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