Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing
Abstract Background The study of soil environment in drainage fields is important for environmental management and ecological restoration, and there is currently a knowledge gap in understanding the impact of soil microbial communities in the Shengli coalfield drainage fields and the corresponding e...
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BMC
2025-03-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-025-03761-7 |
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| author | Weixuan Zhao Ruihong Hou Mingjian Liu Haowei Shen Xiaochen Deng Mingjiu Wang Xiangjun Yun |
| author_facet | Weixuan Zhao Ruihong Hou Mingjian Liu Haowei Shen Xiaochen Deng Mingjiu Wang Xiangjun Yun |
| author_sort | Weixuan Zhao |
| collection | DOAJ |
| description | Abstract Background The study of soil environment in drainage fields is important for environmental management and ecological restoration, and there is currently a knowledge gap in understanding the impact of soil microbial communities in the Shengli coalfield drainage fields and the corresponding ecological effects. To investigate the changes in rhizosphere soil microbial communities of different dominant plants after years of restoration, this study examines the improvement effects of different dominant plants on the soil environment. Results This study is based on high-throughput sequencing to restore the slope of coal mine spoil after 15 years as the sampling site. The rhizosphere soil of five dominant plants was selected for microbial community analysis, and functional prediction of the microbial community was conducted. The dominant plants selected included Erect Milkvetch (Astragalus adsurgens), Lemongrass (Caragana korshinskii), Alfalfa (Medicago sativa), Phyllanthus pinnatifida (Elymus dahuricus), and Brassica Rapa (Brassica campestris). The results showed that after 15 years of restoration, the soil physicochemical properties in the Phyllanthus pinnatifida group were better than those in the other groups overall, but some of them were inferior to those in the lemon-stripped mallard group. Abundant saprophytic fungal communities were found in different dominant plant groups, mainly belonging to the phyla Ascomycota and Basidiomycota, resulting in significantly higher organic matter content in the dominant plant groups compared to the CK group. The bacterial communities were dominated by the phyla Actinobacteriota, Proteobacteria, Chloroflexi, and Firmicutes. Among these microbial phyla, the Phyllanthus pinnatifida group had higher abundance, which is beneficial for vegetation colonization. Redundancy analysis showed that soil pH was significantly correlated with microbial communities. Organic matter content and pH are the main factors influencing the composition of soil microbial communities, significantly affecting the composition of microorganisms in different groups. After years of restoration, the environment of the Shengli Coalfield’s spoil heap has been greatly improved. Conclusions The planting of various beneficial plants has resulted in significant improvements to the soil microbial community and physicochemical properties, with Phyllanthus pinnatifida having the most positive impact. This lays the foundation for the subsequent restoration of the slope of the spoil heap. |
| format | Article |
| id | doaj-art-fa842571a29941fba11ec1f28a2bdf23 |
| institution | DOAJ |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-fa842571a29941fba11ec1f28a2bdf232025-08-20T02:56:08ZengBMCBMC Microbiology1471-21802025-03-0125111810.1186/s12866-025-03761-7Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencingWeixuan Zhao0Ruihong Hou1Mingjian Liu2Haowei Shen3Xiaochen Deng4Mingjiu Wang5Xiangjun Yun6College of Grassland and Resource Environment, the Key Laboratory of Grassland Resources under the Ministry of Education, Inner Mongolia Agricultural UniversityInstitute of Grassland Research of CAASCollege of Grassland and Resource Environment, the Key Laboratory of Grassland Resources under the Ministry of Education, Inner Mongolia Agricultural UniversityCollege of Grassland and Resource Environment, the Key Laboratory of Grassland Resources under the Ministry of Education, Inner Mongolia Agricultural UniversityCollege of Grassland and Resource Environment, the Key Laboratory of Grassland Resources under the Ministry of Education, Inner Mongolia Agricultural UniversityCollege of Grassland and Resource Environment, the Key Laboratory of Grassland Resources under the Ministry of Education, Inner Mongolia Agricultural UniversityInstitute of Grassland Research of CAASAbstract Background The study of soil environment in drainage fields is important for environmental management and ecological restoration, and there is currently a knowledge gap in understanding the impact of soil microbial communities in the Shengli coalfield drainage fields and the corresponding ecological effects. To investigate the changes in rhizosphere soil microbial communities of different dominant plants after years of restoration, this study examines the improvement effects of different dominant plants on the soil environment. Results This study is based on high-throughput sequencing to restore the slope of coal mine spoil after 15 years as the sampling site. The rhizosphere soil of five dominant plants was selected for microbial community analysis, and functional prediction of the microbial community was conducted. The dominant plants selected included Erect Milkvetch (Astragalus adsurgens), Lemongrass (Caragana korshinskii), Alfalfa (Medicago sativa), Phyllanthus pinnatifida (Elymus dahuricus), and Brassica Rapa (Brassica campestris). The results showed that after 15 years of restoration, the soil physicochemical properties in the Phyllanthus pinnatifida group were better than those in the other groups overall, but some of them were inferior to those in the lemon-stripped mallard group. Abundant saprophytic fungal communities were found in different dominant plant groups, mainly belonging to the phyla Ascomycota and Basidiomycota, resulting in significantly higher organic matter content in the dominant plant groups compared to the CK group. The bacterial communities were dominated by the phyla Actinobacteriota, Proteobacteria, Chloroflexi, and Firmicutes. Among these microbial phyla, the Phyllanthus pinnatifida group had higher abundance, which is beneficial for vegetation colonization. Redundancy analysis showed that soil pH was significantly correlated with microbial communities. Organic matter content and pH are the main factors influencing the composition of soil microbial communities, significantly affecting the composition of microorganisms in different groups. After years of restoration, the environment of the Shengli Coalfield’s spoil heap has been greatly improved. Conclusions The planting of various beneficial plants has resulted in significant improvements to the soil microbial community and physicochemical properties, with Phyllanthus pinnatifida having the most positive impact. This lays the foundation for the subsequent restoration of the slope of the spoil heap.https://doi.org/10.1186/s12866-025-03761-7Coal mineDifferent dominant plantsRhizosphere soilMicrobial community characteristicsFunctional prediction |
| spellingShingle | Weixuan Zhao Ruihong Hou Mingjian Liu Haowei Shen Xiaochen Deng Mingjiu Wang Xiangjun Yun Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing BMC Microbiology Coal mine Different dominant plants Rhizosphere soil Microbial community characteristics Functional prediction |
| title | Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing |
| title_full | Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing |
| title_fullStr | Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing |
| title_full_unstemmed | Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing |
| title_short | Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing |
| title_sort | analysis of soil microbial community structure changes in the drainage field of the shengli coalfield based on high throughput sequencing |
| topic | Coal mine Different dominant plants Rhizosphere soil Microbial community characteristics Functional prediction |
| url | https://doi.org/10.1186/s12866-025-03761-7 |
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