Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces
Antimicrobial-resistant Escherichia coli, especially those belonging to the serotype O157, are increasingly linked to foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on E. coli O157:H7 due to its ability to produce toxins coupled with its lo...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/ijm/5087461 |
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| author | Bukola Opeyemi Oluwarinde Daniel Jesuwenu Ajose Tesleem Olatunde Abolarinwa Peter Kotsoana Montso Henry Akum Njom Collins Njie Ateba |
| author_facet | Bukola Opeyemi Oluwarinde Daniel Jesuwenu Ajose Tesleem Olatunde Abolarinwa Peter Kotsoana Montso Henry Akum Njom Collins Njie Ateba |
| author_sort | Bukola Opeyemi Oluwarinde |
| collection | DOAJ |
| description | Antimicrobial-resistant Escherichia coli, especially those belonging to the serotype O157, are increasingly linked to foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on E. coli O157:H7 due to its ability to produce toxins coupled with its low infectious dose. The aim of this study was to assess the virulome, resistome, and pathogenicity of E. coli O157:H7 using whole genome sequencing. Three previously isolated E. coli O157:H7 strains from cattle feces were subjected to whole genome sequencing. The genome sizes of all three E. coli O157:H7 strains were 5,117,276 bp, 5,039,443 bp, and 5,034,351 bp. The C + G contents were 50.22%, 50.53%, and 50.54%, while the number of contigs was 110, 43, and 42, respectively, for E. coli O157:H7 strains J32, J57, and J69. Several virulence determinants (hemorrhagic E. coli pilus (HCP), eaeA, hemolysin, etc.) were found in the genomes of these isolates. In addition, antibiotic resistance genes conferring resistance to aminoglycosides, tetracyclines, macrolides, fluoroquinolones, penams, carbapenems, cephalosporins, cephamycin, rifamycin, phenicols, monobactams, and nitroimidazole were found in the genomes. Interestingly, the genomes of these isolates also harbored determinants encoding resistance to disinfectants and antiseptics, indicating their concern in the food production and medical sectors. This highlights the public health concerns of these isolates, indicating the need for constant surveillance. |
| format | Article |
| id | doaj-art-fa8315c04cbd4e8f90d0a30817acd7db |
| institution | OA Journals |
| issn | 1687-9198 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-fa8315c04cbd4e8f90d0a30817acd7db2025-08-20T02:13:27ZengWileyInternational Journal of Microbiology1687-91982025-01-01202510.1155/ijm/5087461Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle FecesBukola Opeyemi Oluwarinde0Daniel Jesuwenu Ajose1Tesleem Olatunde Abolarinwa2Peter Kotsoana Montso3Henry Akum Njom4Collins Njie Ateba5Food Security and Safety Focus AreaFood Security and Safety Focus AreaFood Security and Safety Focus AreaFood Security and Safety Focus AreaAgricultural Research CouncilFood Security and Safety Focus AreaAntimicrobial-resistant Escherichia coli, especially those belonging to the serotype O157, are increasingly linked to foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on E. coli O157:H7 due to its ability to produce toxins coupled with its low infectious dose. The aim of this study was to assess the virulome, resistome, and pathogenicity of E. coli O157:H7 using whole genome sequencing. Three previously isolated E. coli O157:H7 strains from cattle feces were subjected to whole genome sequencing. The genome sizes of all three E. coli O157:H7 strains were 5,117,276 bp, 5,039,443 bp, and 5,034,351 bp. The C + G contents were 50.22%, 50.53%, and 50.54%, while the number of contigs was 110, 43, and 42, respectively, for E. coli O157:H7 strains J32, J57, and J69. Several virulence determinants (hemorrhagic E. coli pilus (HCP), eaeA, hemolysin, etc.) were found in the genomes of these isolates. In addition, antibiotic resistance genes conferring resistance to aminoglycosides, tetracyclines, macrolides, fluoroquinolones, penams, carbapenems, cephalosporins, cephamycin, rifamycin, phenicols, monobactams, and nitroimidazole were found in the genomes. Interestingly, the genomes of these isolates also harbored determinants encoding resistance to disinfectants and antiseptics, indicating their concern in the food production and medical sectors. This highlights the public health concerns of these isolates, indicating the need for constant surveillance.http://dx.doi.org/10.1155/ijm/5087461 |
| spellingShingle | Bukola Opeyemi Oluwarinde Daniel Jesuwenu Ajose Tesleem Olatunde Abolarinwa Peter Kotsoana Montso Henry Akum Njom Collins Njie Ateba Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces International Journal of Microbiology |
| title | Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces |
| title_full | Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces |
| title_fullStr | Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces |
| title_full_unstemmed | Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces |
| title_short | Unraveling the Resistome, Virulome, and Pathogenicity of Escherichia Coli O157:H7 From Cattle Feces |
| title_sort | unraveling the resistome virulome and pathogenicity of escherichia coli o157 h7 from cattle feces |
| url | http://dx.doi.org/10.1155/ijm/5087461 |
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