NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES
PURPOSE: To compare the effectiveness of a newly designed multi-component cryoprotective medium (15% PEO-1500, 10% DMSO, 5% 1.2-propanediol, 5% sucrose) with three known singleand two-component ones containing DMSO (20 %), PEO-1500 (30 %), and glycerol-mannitol (30 % and 4 %, respectively) in the cr...
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| Format: | Article |
| Language: | English |
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Trakia University
2019-12-01
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| Series: | Trakia Journal of Sciences |
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| Online Access: | http://tru.uni-sz.bg/tsj/vol17,sp4-2019/1.pdf |
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| author | P. Ulizko O. Bobrova O. Nardid L. Vodopyanova S. Repina |
| author_facet | P. Ulizko O. Bobrova O. Nardid L. Vodopyanova S. Repina |
| author_sort | P. Ulizko |
| collection | DOAJ |
| description | PURPOSE: To compare the effectiveness of a newly designed multi-component cryoprotective medium (15% PEO-1500, 10% DMSO, 5% 1.2-propanediol, 5% sucrose) with three known singleand two-component ones containing DMSO (20 %), PEO-1500 (30 %), and glycerol-mannitol (30 % and 4 %, respectively) in the cryopreservation of human, equine, bovine and rabbit erythrocytes.
METHODS: Cryopreservation was achieved plunging the cryotubes with erythrocyte suspensions into liquid nitrogen (-196°C) at an average cooling rate of 200 deg/min. During the three cryopreservation stages; equilibration, freeze-thaw and final removal of cryoprotectants, the survival of erythrocytes
was estimated by the level of hemolysis, measured spectrophotometrically. RESULTS: With the erythrocytes of all investigated mammals the designed medium produced a higher level of survival at all cryopreservation stages. CONCLUSION: The tested multi-component cryopreservation medium,
combining both penetrating and non-penetrating cryoprotectants, diminished their concentration and accompanying toxic effects and achieved a higher survival of human and mammal erythrocytes at all stages of their cryopreservation. |
| format | Article |
| id | doaj-art-fa813973a98940ad8c197467c8709cbc |
| institution | DOAJ |
| issn | 1312-1723 1313-3551 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Trakia University |
| record_format | Article |
| series | Trakia Journal of Sciences |
| spelling | doaj-art-fa813973a98940ad8c197467c8709cbc2025-08-20T02:55:02ZengTrakia UniversityTrakia Journal of Sciences1312-17231313-35512019-12-0117430330710.15547/tjs.2019.04.001NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTESP. UlizkoO. BobrovaO. NardidL. VodopyanovaS. RepinaPURPOSE: To compare the effectiveness of a newly designed multi-component cryoprotective medium (15% PEO-1500, 10% DMSO, 5% 1.2-propanediol, 5% sucrose) with three known singleand two-component ones containing DMSO (20 %), PEO-1500 (30 %), and glycerol-mannitol (30 % and 4 %, respectively) in the cryopreservation of human, equine, bovine and rabbit erythrocytes. METHODS: Cryopreservation was achieved plunging the cryotubes with erythrocyte suspensions into liquid nitrogen (-196°C) at an average cooling rate of 200 deg/min. During the three cryopreservation stages; equilibration, freeze-thaw and final removal of cryoprotectants, the survival of erythrocytes was estimated by the level of hemolysis, measured spectrophotometrically. RESULTS: With the erythrocytes of all investigated mammals the designed medium produced a higher level of survival at all cryopreservation stages. CONCLUSION: The tested multi-component cryopreservation medium, combining both penetrating and non-penetrating cryoprotectants, diminished their concentration and accompanying toxic effects and achieved a higher survival of human and mammal erythrocytes at all stages of their cryopreservation.http://tru.uni-sz.bg/tsj/vol17,sp4-2019/1.pdfhumanbovineequine and rabbit red blood cellspenetrating and non-penetrating cryoprotectantssurvivalhemolysis |
| spellingShingle | P. Ulizko O. Bobrova O. Nardid L. Vodopyanova S. Repina NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES Trakia Journal of Sciences human bovine equine and rabbit red blood cells penetrating and non-penetrating cryoprotectants survival hemolysis |
| title | NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES |
| title_full | NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES |
| title_fullStr | NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES |
| title_full_unstemmed | NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES |
| title_short | NEW CRYOPROTECTIVE MEDIA FOR CRYOPRESERVATION OF MAMMAL ERYTHROCYTES |
| title_sort | new cryoprotective media for cryopreservation of mammal erythrocytes |
| topic | human bovine equine and rabbit red blood cells penetrating and non-penetrating cryoprotectants survival hemolysis |
| url | http://tru.uni-sz.bg/tsj/vol17,sp4-2019/1.pdf |
| work_keys_str_mv | AT pulizko newcryoprotectivemediaforcryopreservationofmammalerythrocytes AT obobrova newcryoprotectivemediaforcryopreservationofmammalerythrocytes AT onardid newcryoprotectivemediaforcryopreservationofmammalerythrocytes AT lvodopyanova newcryoprotectivemediaforcryopreservationofmammalerythrocytes AT srepina newcryoprotectivemediaforcryopreservationofmammalerythrocytes |