RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.

<h4>Background</h4>The strong familiality of living to extreme ages suggests that human longevity is genetically regulated. The majority of genes found thus far to be associated with longevity primarily function in lipoprotein metabolism and insulin/IGF-1 signaling. There are likely many...

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Main Authors: Paola Sebastiani, Monty Montano, Annibale Puca, Nadia Solovieff, Toshio Kojima, Meng C Wang, Efthymia Melista, Micah Meltzer, Sylvia E J Fischer, Stacy Andersen, Stephen H Hartley, Amanda Sedgewick, Yasumichi Arai, Aviv Bergman, Nir Barzilai, Dellara F Terry, Alberto Riva, Chiara Viviani Anselmi, Alberto Malovini, Aya Kitamoto, Motoji Sawabe, Tomio Arai, Yasuyuki Gondo, Martin H Steinberg, Nobuyoshi Hirose, Gil Atzmon, Gary Ruvkun, Clinton T Baldwin, Thomas T Perls
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-12-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0008210&type=printable
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author Paola Sebastiani
Monty Montano
Annibale Puca
Nadia Solovieff
Toshio Kojima
Meng C Wang
Efthymia Melista
Micah Meltzer
Sylvia E J Fischer
Stacy Andersen
Stephen H Hartley
Amanda Sedgewick
Yasumichi Arai
Aviv Bergman
Nir Barzilai
Dellara F Terry
Alberto Riva
Chiara Viviani Anselmi
Alberto Malovini
Aya Kitamoto
Motoji Sawabe
Tomio Arai
Yasuyuki Gondo
Martin H Steinberg
Nobuyoshi Hirose
Gil Atzmon
Gary Ruvkun
Clinton T Baldwin
Thomas T Perls
author_facet Paola Sebastiani
Monty Montano
Annibale Puca
Nadia Solovieff
Toshio Kojima
Meng C Wang
Efthymia Melista
Micah Meltzer
Sylvia E J Fischer
Stacy Andersen
Stephen H Hartley
Amanda Sedgewick
Yasumichi Arai
Aviv Bergman
Nir Barzilai
Dellara F Terry
Alberto Riva
Chiara Viviani Anselmi
Alberto Malovini
Aya Kitamoto
Motoji Sawabe
Tomio Arai
Yasuyuki Gondo
Martin H Steinberg
Nobuyoshi Hirose
Gil Atzmon
Gary Ruvkun
Clinton T Baldwin
Thomas T Perls
author_sort Paola Sebastiani
collection DOAJ
description <h4>Background</h4>The strong familiality of living to extreme ages suggests that human longevity is genetically regulated. The majority of genes found thus far to be associated with longevity primarily function in lipoprotein metabolism and insulin/IGF-1 signaling. There are likely many more genetic modifiers of human longevity that remain to be discovered.<h4>Methodology/principal findings</h4>Here, we first show that 18 single nucleotide polymorphisms (SNPs) in the RNA editing genes ADARB1 and ADARB2 are associated with extreme old age in a U.S. based study of centenarians, the New England Centenarian Study. We describe replications of these findings in three independently conducted centenarian studies with different genetic backgrounds (Italian, Ashkenazi Jewish and Japanese) that collectively support an association of ADARB1 and ADARB2 with longevity. Some SNPs in ADARB2 replicate consistently in the four populations and suggest a strong effect that is independent of the different genetic backgrounds and environments. To evaluate the functional association of these genes with lifespan, we demonstrate that inactivation of their orthologues adr-1 and adr-2 in C. elegans reduces median survival by 50%. We further demonstrate that inactivation of the argonaute gene, rde-1, a critical regulator of RNA interference, completely restores lifespan to normal levels in the context of adr-1 and adr-2 loss of function.<h4>Conclusions/significance</h4>Our results suggest that RNA editors may be an important regulator of aging in humans and that, when evaluated in C. elegans, this pathway may interact with the RNA interference machinery to regulate lifespan.
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spelling doaj-art-fa7f218462b9403da2649f61a12fb2b82025-08-20T02:01:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-12-01412e821010.1371/journal.pone.0008210RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.Paola SebastianiMonty MontanoAnnibale PucaNadia SolovieffToshio KojimaMeng C WangEfthymia MelistaMicah MeltzerSylvia E J FischerStacy AndersenStephen H HartleyAmanda SedgewickYasumichi AraiAviv BergmanNir BarzilaiDellara F TerryAlberto RivaChiara Viviani AnselmiAlberto MaloviniAya KitamotoMotoji SawabeTomio AraiYasuyuki GondoMartin H SteinbergNobuyoshi HiroseGil AtzmonGary RuvkunClinton T BaldwinThomas T Perls<h4>Background</h4>The strong familiality of living to extreme ages suggests that human longevity is genetically regulated. The majority of genes found thus far to be associated with longevity primarily function in lipoprotein metabolism and insulin/IGF-1 signaling. There are likely many more genetic modifiers of human longevity that remain to be discovered.<h4>Methodology/principal findings</h4>Here, we first show that 18 single nucleotide polymorphisms (SNPs) in the RNA editing genes ADARB1 and ADARB2 are associated with extreme old age in a U.S. based study of centenarians, the New England Centenarian Study. We describe replications of these findings in three independently conducted centenarian studies with different genetic backgrounds (Italian, Ashkenazi Jewish and Japanese) that collectively support an association of ADARB1 and ADARB2 with longevity. Some SNPs in ADARB2 replicate consistently in the four populations and suggest a strong effect that is independent of the different genetic backgrounds and environments. To evaluate the functional association of these genes with lifespan, we demonstrate that inactivation of their orthologues adr-1 and adr-2 in C. elegans reduces median survival by 50%. We further demonstrate that inactivation of the argonaute gene, rde-1, a critical regulator of RNA interference, completely restores lifespan to normal levels in the context of adr-1 and adr-2 loss of function.<h4>Conclusions/significance</h4>Our results suggest that RNA editors may be an important regulator of aging in humans and that, when evaluated in C. elegans, this pathway may interact with the RNA interference machinery to regulate lifespan.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0008210&type=printable
spellingShingle Paola Sebastiani
Monty Montano
Annibale Puca
Nadia Solovieff
Toshio Kojima
Meng C Wang
Efthymia Melista
Micah Meltzer
Sylvia E J Fischer
Stacy Andersen
Stephen H Hartley
Amanda Sedgewick
Yasumichi Arai
Aviv Bergman
Nir Barzilai
Dellara F Terry
Alberto Riva
Chiara Viviani Anselmi
Alberto Malovini
Aya Kitamoto
Motoji Sawabe
Tomio Arai
Yasuyuki Gondo
Martin H Steinberg
Nobuyoshi Hirose
Gil Atzmon
Gary Ruvkun
Clinton T Baldwin
Thomas T Perls
RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.
PLoS ONE
title RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.
title_full RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.
title_fullStr RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.
title_full_unstemmed RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.
title_short RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans.
title_sort rna editing genes associated with extreme old age in humans and with lifespan in c elegans
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0008210&type=printable
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