T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol

T-2 toxin, classified as a mycotoxin, harms immune and cognitive systems. Recently, an increasing number of studies have focused on the neurotoxic effects of T-2 toxin. However, there is still a need for a better understanding of the molecular mechanisms involved. In this study, we aimed to elucidat...

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Main Authors: Sheng-Quan Su, Gui-Yuan Xiong, Chun-Yan Yao, Xiao-Ling Liu, Yin-Yin Xia, Jin-Yun Long, Xiu-Kuan Li, Liang-Mei Wang, Long Yi, Wu-Wei Xu, Wu-Qiang Lu, Qian-Qian Li, Ting Hu, Hao Liu, Ke-Xue Wang, Peng Luo, Tong-Jian Cai
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325009686
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author Sheng-Quan Su
Gui-Yuan Xiong
Chun-Yan Yao
Xiao-Ling Liu
Yin-Yin Xia
Jin-Yun Long
Xiu-Kuan Li
Liang-Mei Wang
Long Yi
Wu-Wei Xu
Wu-Qiang Lu
Qian-Qian Li
Ting Hu
Hao Liu
Ke-Xue Wang
Peng Luo
Tong-Jian Cai
author_facet Sheng-Quan Su
Gui-Yuan Xiong
Chun-Yan Yao
Xiao-Ling Liu
Yin-Yin Xia
Jin-Yun Long
Xiu-Kuan Li
Liang-Mei Wang
Long Yi
Wu-Wei Xu
Wu-Qiang Lu
Qian-Qian Li
Ting Hu
Hao Liu
Ke-Xue Wang
Peng Luo
Tong-Jian Cai
author_sort Sheng-Quan Su
collection DOAJ
description T-2 toxin, classified as a mycotoxin, harms immune and cognitive systems. Recently, an increasing number of studies have focused on the neurotoxic effects of T-2 toxin. However, there is still a need for a better understanding of the molecular mechanisms involved. In this study, we aimed to elucidate the protective effect of resveratrol (Res) supplementation against T-2 toxin-induced spatial learning and memory impairment and to investigate the communication mechanisms between the gut and the brain. Mice received a single intraperitoneal dose of T-2 toxin (4 mg/kg) followed by Res (100 mg/kg) administered via the same route. The Morris water maze and novel object recognition tests demonstrated that T-2 toxin had a profound negative impact on spatial learning and memory in mice. However, the administration of Res successfully mitigated the cognitive impairments caused by T-2 toxin and notably enhanced the levels of brain-derived neurotrophic factor (BDNF), synaptophysin I (SYN1), and postsynaptic density protein 95 (PSD-95). Moreover, Res demonstrated a protective effect against T-2 toxin-induced damage to hippocampal tissue. Simultaneously, it played a pivotal role in restoring gut microbiota equilibrium and maintaining the integrity of intestinal tight junctions, effectively curbing both localized and systemic inflammation triggered by T-2 toxin exposure. In conclusion, our findings suggest that resveratrol may alleviate T-2 toxin-induced spatial learning and memory deficits, possibly via gut-brain axis modulation.
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spelling doaj-art-fa7c77a40af341e39071e22be2c6f35e2025-08-20T04:02:31ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211862310.1016/j.ecoenv.2025.118623T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrolSheng-Quan Su0Gui-Yuan Xiong1Chun-Yan Yao2Xiao-Ling Liu3Yin-Yin Xia4Jin-Yun Long5Xiu-Kuan Li6Liang-Mei Wang7Long Yi8Wu-Wei Xu9Wu-Qiang Lu10Qian-Qian Li11Ting Hu12Hao Liu13Ke-Xue Wang14Peng Luo15Tong-Jian Cai16School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China; Department of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China; Department of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaDepartment of Epidemiology, College of Preventive Medicine, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400038, ChinaDepartment of Epidemiology, College of Preventive Medicine, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400038, ChinaSchool of Public Health, Chongqing Medical University, Chongqing 400016, ChinaChongqing Jiangjin District Center for Disease Control and Prevention, Chongqing 402260, ChinaShizong County People's Hospital, Shizong 655700, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China; Department of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaResearch Center for Nutrition and Food Safety, College of Preventive Medicine, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400038, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China; Department of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaDepartment of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaDepartment of Epidemiology, College of Preventive Medicine, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400038, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, ChinaDepartment of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaDepartment of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 550014, China; Corresponding author at: School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China.Department of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Chongqing 400042, China; School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China; Correspondence to: Department of Disease Prevention and Control, Daping Hospital, State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Third Military Medical University), Changjiang Branch St, 10#, Yuzhong, Chongqing 400042, China.T-2 toxin, classified as a mycotoxin, harms immune and cognitive systems. Recently, an increasing number of studies have focused on the neurotoxic effects of T-2 toxin. However, there is still a need for a better understanding of the molecular mechanisms involved. In this study, we aimed to elucidate the protective effect of resveratrol (Res) supplementation against T-2 toxin-induced spatial learning and memory impairment and to investigate the communication mechanisms between the gut and the brain. Mice received a single intraperitoneal dose of T-2 toxin (4 mg/kg) followed by Res (100 mg/kg) administered via the same route. The Morris water maze and novel object recognition tests demonstrated that T-2 toxin had a profound negative impact on spatial learning and memory in mice. However, the administration of Res successfully mitigated the cognitive impairments caused by T-2 toxin and notably enhanced the levels of brain-derived neurotrophic factor (BDNF), synaptophysin I (SYN1), and postsynaptic density protein 95 (PSD-95). Moreover, Res demonstrated a protective effect against T-2 toxin-induced damage to hippocampal tissue. Simultaneously, it played a pivotal role in restoring gut microbiota equilibrium and maintaining the integrity of intestinal tight junctions, effectively curbing both localized and systemic inflammation triggered by T-2 toxin exposure. In conclusion, our findings suggest that resveratrol may alleviate T-2 toxin-induced spatial learning and memory deficits, possibly via gut-brain axis modulation.http://www.sciencedirect.com/science/article/pii/S0147651325009686T-2 toxinResveratrolGut-Brain axisIntestinal microbiotaCognitive impairmentHippocampus
spellingShingle Sheng-Quan Su
Gui-Yuan Xiong
Chun-Yan Yao
Xiao-Ling Liu
Yin-Yin Xia
Jin-Yun Long
Xiu-Kuan Li
Liang-Mei Wang
Long Yi
Wu-Wei Xu
Wu-Qiang Lu
Qian-Qian Li
Ting Hu
Hao Liu
Ke-Xue Wang
Peng Luo
Tong-Jian Cai
T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol
Ecotoxicology and Environmental Safety
T-2 toxin
Resveratrol
Gut-Brain axis
Intestinal microbiota
Cognitive impairment
Hippocampus
title T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol
title_full T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol
title_fullStr T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol
title_full_unstemmed T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol
title_short T-2 toxin-induced spatial learning and memory impairment in mice via the gut-brain axis and the intervening effects of resveratrol
title_sort t 2 toxin induced spatial learning and memory impairment in mice via the gut brain axis and the intervening effects of resveratrol
topic T-2 toxin
Resveratrol
Gut-Brain axis
Intestinal microbiota
Cognitive impairment
Hippocampus
url http://www.sciencedirect.com/science/article/pii/S0147651325009686
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