Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy
In order to improve the efficiency of monitoring and detecting the residues of the anticoccidial veterinary drug lasalocid (LAS) in chicken, a sensitive method for the determination of LAS residues was established by surface-enhanced Raman spectroscopy (SERS). In this study, gold nanoparticles (AuNP...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030216 |
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| author | Yao WANG Yingying SUN Yubing JING Xiyan YANG Yanfei LI Jiaxiang HUANG Xiujin CHEN Zhaozhou LI Li CAO Huaibin KANG |
| author_facet | Yao WANG Yingying SUN Yubing JING Xiyan YANG Yanfei LI Jiaxiang HUANG Xiujin CHEN Zhaozhou LI Li CAO Huaibin KANG |
| author_sort | Yao WANG |
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| description | In order to improve the efficiency of monitoring and detecting the residues of the anticoccidial veterinary drug lasalocid (LAS) in chicken, a sensitive method for the determination of LAS residues was established by surface-enhanced Raman spectroscopy (SERS). In this study, gold nanoparticles (AuNPs) with different particle sizes were first prepared, AuNPs with the optimal particle size were selected as the substrate according to SERS effect, and 4-aminophenthiophenol (PATP) was used as the probe molecule to evaluate the recurrence of AuNPs substrate. Then, by adding HNO3, AuNPs aggregation degree was changed and LAS solubility was increased to further enhance SERS signal. Finally, the method was evaluated by establishing the standard curve and the recovery test of chicken sample. Results showed that AuNPs with a particle size of 40 nm had good enhancement effect, the SERS characteristic peak of LAS was at 1118 cm−1, the AuNPs substrate had good reproducibility, and the optimal addition concentration of HNO3 was 0.5 mol/L. The linear range of this method was 0.55×10−6~0.55 mg/mL, the detection limit was 0.23 ng/mL, the recovery rate of chicken samples was 91.9%~107.3%, and the relative standard deviation was 3.6%~5.7%, which was consistent with the detection results of the national standard method (GB31613.5-2022). This study provides a new method for the sensitive detection of LAS residues, and has reference value for the study of SERS detection method of other veterinary drug residues. |
| format | Article |
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| institution | Kabale University |
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| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-fa6f2cbe3e0a4abcb9c63233321691832025-02-08T08:06:21ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146429930510.13386/j.issn1002-0306.20240302162024030216-4Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman SpectroscopyYao WANG0Yingying SUN1Yubing JING2Xiyan YANG3Yanfei LI4Jiaxiang HUANG5Xiujin CHEN6Zhaozhou LI7Li CAO8Huaibin KANG9College of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Food and Bioengineering/National Demonstration Center for Experimental Food Processing and Safety Education/Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471000, ChinaIn order to improve the efficiency of monitoring and detecting the residues of the anticoccidial veterinary drug lasalocid (LAS) in chicken, a sensitive method for the determination of LAS residues was established by surface-enhanced Raman spectroscopy (SERS). In this study, gold nanoparticles (AuNPs) with different particle sizes were first prepared, AuNPs with the optimal particle size were selected as the substrate according to SERS effect, and 4-aminophenthiophenol (PATP) was used as the probe molecule to evaluate the recurrence of AuNPs substrate. Then, by adding HNO3, AuNPs aggregation degree was changed and LAS solubility was increased to further enhance SERS signal. Finally, the method was evaluated by establishing the standard curve and the recovery test of chicken sample. Results showed that AuNPs with a particle size of 40 nm had good enhancement effect, the SERS characteristic peak of LAS was at 1118 cm−1, the AuNPs substrate had good reproducibility, and the optimal addition concentration of HNO3 was 0.5 mol/L. The linear range of this method was 0.55×10−6~0.55 mg/mL, the detection limit was 0.23 ng/mL, the recovery rate of chicken samples was 91.9%~107.3%, and the relative standard deviation was 3.6%~5.7%, which was consistent with the detection results of the national standard method (GB31613.5-2022). This study provides a new method for the sensitive detection of LAS residues, and has reference value for the study of SERS detection method of other veterinary drug residues.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030216surface enhanced raman spectroscopylasalocidgold nanoparticleschickendetection |
| spellingShingle | Yao WANG Yingying SUN Yubing JING Xiyan YANG Yanfei LI Jiaxiang HUANG Xiujin CHEN Zhaozhou LI Li CAO Huaibin KANG Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy Shipin gongye ke-ji surface enhanced raman spectroscopy lasalocid gold nanoparticles chicken detection |
| title | Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy |
| title_full | Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy |
| title_fullStr | Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy |
| title_full_unstemmed | Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy |
| title_short | Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy |
| title_sort | sensitive detection of lasalocid residue in chicken by surface enhanced raman spectroscopy |
| topic | surface enhanced raman spectroscopy lasalocid gold nanoparticles chicken detection |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030216 |
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