Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis
<i>Pseudomonas aeruginosa</i> otitis is common in dogs and antibiotic-resistant strains are often isolated. We are unaware of reports evaluating the combination of silver sulfadiazine (SSD) with a biofilm disruptor solution containing Tris-EDTA (tromethamine-ethylenediaminetetraacetic ac...
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2025-07-01
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| author | Ioanna Papadogiannaki Rosario Cerundolo Jennifer Plant Elizabeth Villiers Jenny Littler Anika Wisniewska Panagiotis Sgardelis |
| author_facet | Ioanna Papadogiannaki Rosario Cerundolo Jennifer Plant Elizabeth Villiers Jenny Littler Anika Wisniewska Panagiotis Sgardelis |
| author_sort | Ioanna Papadogiannaki |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> otitis is common in dogs and antibiotic-resistant strains are often isolated. We are unaware of reports evaluating the combination of silver sulfadiazine (SSD) with a biofilm disruptor solution containing Tris-EDTA (tromethamine-ethylenediaminetetraacetic acid) and N-acetylcysteine (Tris-NAC). Forty-eight <i>P. aeruginosa</i> strains from dogs with suppurative otitis were analysed using the agar well diffusion method. A volume of 70 μL of Tris-NAC, a water solution of 10% SSD and their combination in equal amount was pipetted into the designated wells. After incubation, the diameter of the inhibition zone was measured. A synergy experiment using the checkerboard assay was performed to look at any potential synergistic effects of SSD and Tris-NAC against only 10 randomly selected isolates of <i>P. aeruginosa</i>. The samples tested with Tris-NAC + 10% SSD solution, compared with the samples tested with 10% SSD alone, demonstrated significantly higher inhibition zones against <i>P. aeruginosa</i>, <i>p</i> < 0.00001. The checkerboard assay results showed an additive effect between the two compounds. The use of 10% SSD could be evaluated as a therapeutic tool against strains of <i>P. aeruginosa</i> if combined with Tris-NAC. |
| format | Article |
| id | doaj-art-fa6734db77f44e31989254ca9c871f12 |
| institution | DOAJ |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-fa6734db77f44e31989254ca9c871f122025-08-20T02:47:04ZengMDPI AGMicrobiology Research2036-74812025-07-0116713810.3390/microbiolres16070138Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative OtitisIoanna Papadogiannaki0Rosario Cerundolo1Jennifer Plant2Elizabeth Villiers3Jenny Littler4Anika Wisniewska5Panagiotis Sgardelis6Dermatology Section, Dick White Referrals, Veterinary Specialist Centre, Station Farm, London Road, Six Mile Bottom, Suffolk CB8 0UH, UKDermatology Section, Dick White Referrals, Veterinary Specialist Centre, Station Farm, London Road, Six Mile Bottom, Suffolk CB8 0UH, UKMicrobiology Section, Antech Laboratory, Dick White Referrals, Veterinary Specialist Centre, Station Farm, London Road, Six Mile Bottom, Suffolk CB8 0UH, UKMicrobiology Section, Antech Laboratory, Dick White Referrals, Veterinary Specialist Centre, Station Farm, London Road, Six Mile Bottom, Suffolk CB8 0UH, UKWarwick Antimicrobial Screening Facility, University of Warwick, Interdisciplinary Biomedical Research Building, Gibbet Hill, Coventry CV4 7AL, UKMicrobiology and Molecular Biology, Antech Diagnostics Limited, Unit 1, Titan Business Centre, Tachbrook Park, Warwick CV34 6RR, UKNorfolk & Norwich University Hospital, Colney Ln, Norwich NR4 7UY, UK<i>Pseudomonas aeruginosa</i> otitis is common in dogs and antibiotic-resistant strains are often isolated. We are unaware of reports evaluating the combination of silver sulfadiazine (SSD) with a biofilm disruptor solution containing Tris-EDTA (tromethamine-ethylenediaminetetraacetic acid) and N-acetylcysteine (Tris-NAC). Forty-eight <i>P. aeruginosa</i> strains from dogs with suppurative otitis were analysed using the agar well diffusion method. A volume of 70 μL of Tris-NAC, a water solution of 10% SSD and their combination in equal amount was pipetted into the designated wells. After incubation, the diameter of the inhibition zone was measured. A synergy experiment using the checkerboard assay was performed to look at any potential synergistic effects of SSD and Tris-NAC against only 10 randomly selected isolates of <i>P. aeruginosa</i>. The samples tested with Tris-NAC + 10% SSD solution, compared with the samples tested with 10% SSD alone, demonstrated significantly higher inhibition zones against <i>P. aeruginosa</i>, <i>p</i> < 0.00001. The checkerboard assay results showed an additive effect between the two compounds. The use of 10% SSD could be evaluated as a therapeutic tool against strains of <i>P. aeruginosa</i> if combined with Tris-NAC.https://www.mdpi.com/2036-7481/16/7/138dogN-acetylcysteineotitis<i>Pseudomonas aeruginosa</i>silver sulfadiazineTris-EDTA |
| spellingShingle | Ioanna Papadogiannaki Rosario Cerundolo Jennifer Plant Elizabeth Villiers Jenny Littler Anika Wisniewska Panagiotis Sgardelis Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis Microbiology Research dog N-acetylcysteine otitis <i>Pseudomonas aeruginosa</i> silver sulfadiazine Tris-EDTA |
| title | Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis |
| title_full | Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis |
| title_fullStr | Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis |
| title_full_unstemmed | Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis |
| title_short | Antibacterial In Vitro Properties of Silver Sulfadiazine in Combination with Tris-EDTA and N-Acetylcysteine Against <i>Pseudomonas aeruginosa</i> Isolates from Dogs with Suppurative Otitis |
| title_sort | antibacterial in vitro properties of silver sulfadiazine in combination with tris edta and n acetylcysteine against i pseudomonas aeruginosa i isolates from dogs with suppurative otitis |
| topic | dog N-acetylcysteine otitis <i>Pseudomonas aeruginosa</i> silver sulfadiazine Tris-EDTA |
| url | https://www.mdpi.com/2036-7481/16/7/138 |
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