Antibiofilm activity of pyocin against local isolates of Pseudomonas aeruginosa
Background: Pyocins are narrow-spectrum bacteriocins that kill bacteria closely related to the producing strain and play a key role in colonization and competition in bacterial communities. This study aims to investigate the antibacterial and antibiofilm activity of pyocin. Methodology: Twenty bact...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Al-Nahrain University/ Biotechnology Research Center
2025-07-01
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| Series: | مجلة مركز بحوث التقنيات الاحيائية |
| Subjects: | |
| Online Access: | https://jobrc.org/index.php/jobrc/article/view/923 |
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| Summary: | Background: Pyocins are narrow-spectrum bacteriocins that kill bacteria closely related to the producing strain and play a key role in colonization and competition in bacterial communities. This study aims to investigate the antibacterial and antibiofilm activity of pyocin. Methodology: Twenty bacterial isolates of Pseudomonas aeruginosa were isolated from wounds and burns; the bacteriocin-producing isolates were detected using the cup assay method. Then, the bacteriocin was extracted from a highly producing strain, and this bacteriocin was tested against bacterial isolates other than the producing strain in both planktonic and biofilm form. The ability to form biofilm was tested using the microtiter plate method to isolate isolates that form strong biofilms. The antibiofilm activity of bacteriocin was also studied in this study using the same procedure as that used for testing biofilm. Results: The results showed that all bacterial isolates possess the ability to produce bacteriocin against each other, but not against their own bacteriocin, because each isolate contains an immunity gene against its bacteriocin. The results of the biofilm study showed that six isolates produced a strong biofilm, while others produced a moderate biofilm. Other findings showed that bacteriocin exhibited high activity against biofilms formed by potent isolates, with the percentage of inhibition ranging from 48 to 67%. Conclusion: The bacteriocin can be used to inhibit biofilm-producing isolates of the same species, making it a potential alternative to antibiotics.
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| ISSN: | 1815-1140 2708-1370 |