Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut

Quorum sensing (QS) mechanisms play an essential role in mediating several signals and plant-bacteria interactions, promoting plant growth. This study demonstrated production of multiple Homoserine lactone (HSL) molecules like C6 HSL, C7 HSL, C8 HSL, 3-Hydroxy-C8-HSL and 3-oxo-C14 HSL in rhizobial a...

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Main Authors: Sivakumar Madhan, Yuvasri Errakutty Arunan, Anandham Rangasamy, Balachandar Dananjeyan, Johnson Iruthayasamy, Manimaran Gajendiran, Krishnamoorthy Ramasamy, Raghu Rajasekaran, Vincent Saminathan
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Biotechnology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X25000207
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author Sivakumar Madhan
Yuvasri Errakutty Arunan
Anandham Rangasamy
Balachandar Dananjeyan
Johnson Iruthayasamy
Manimaran Gajendiran
Krishnamoorthy Ramasamy
Raghu Rajasekaran
Vincent Saminathan
author_facet Sivakumar Madhan
Yuvasri Errakutty Arunan
Anandham Rangasamy
Balachandar Dananjeyan
Johnson Iruthayasamy
Manimaran Gajendiran
Krishnamoorthy Ramasamy
Raghu Rajasekaran
Vincent Saminathan
author_sort Sivakumar Madhan
collection DOAJ
description Quorum sensing (QS) mechanisms play an essential role in mediating several signals and plant-bacteria interactions, promoting plant growth. This study demonstrated production of multiple Homoserine lactone (HSL) molecules like C6 HSL, C7 HSL, C8 HSL, 3-Hydroxy-C8-HSL and 3-oxo-C14 HSL in rhizobial and passenger endophytes and phyllospheric bacteria which regulated production of plant growth promoting traits viz., indole acetic acid and exo-polysaccharide production, biofilm formation, and motility. Quorum quenching (QQ) molecules like salicylic acid, gallic acid, and disalicylic acid impaired these traits, but exogenous addition of QS molecules (C7HSL and 3-oxo-C14 HSL) restored these inhibitory effects of QQ compounds. The pot culture experiment revealed that the treatment involving Methylobacterium populi TMV7–4 or Enterobacter cloacae S23 with salicylic acid, C7HSL and 3-oxo-C14 HSL significantly enhanced plant growth including root length, nodulation, pod formation, soil available nutrients and plant nutrients uptake. In future field validation is required for the use of QS molecules in improving groundnut production.
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spelling doaj-art-fa5f88a7958d41feb8332195b688832c2025-08-20T03:12:39ZengElsevierBiotechnology Reports2215-017X2025-06-0146e0089310.1016/j.btre.2025.e00893Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnutSivakumar Madhan0Yuvasri Errakutty Arunan1Anandham Rangasamy2Balachandar Dananjeyan3Johnson Iruthayasamy4Manimaran Gajendiran5Krishnamoorthy Ramasamy6Raghu Rajasekaran7Vincent Saminathan8Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaDepartment of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaDepartment of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, India; Corresponding author.Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaDepartment of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaDepartment of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaDepartment of Crop Management, Vanavarayar Institute of Agriculture, Pollachi, Tamil Nadu 642103, IndiaCentre for Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaDepartment of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, IndiaQuorum sensing (QS) mechanisms play an essential role in mediating several signals and plant-bacteria interactions, promoting plant growth. This study demonstrated production of multiple Homoserine lactone (HSL) molecules like C6 HSL, C7 HSL, C8 HSL, 3-Hydroxy-C8-HSL and 3-oxo-C14 HSL in rhizobial and passenger endophytes and phyllospheric bacteria which regulated production of plant growth promoting traits viz., indole acetic acid and exo-polysaccharide production, biofilm formation, and motility. Quorum quenching (QQ) molecules like salicylic acid, gallic acid, and disalicylic acid impaired these traits, but exogenous addition of QS molecules (C7HSL and 3-oxo-C14 HSL) restored these inhibitory effects of QQ compounds. The pot culture experiment revealed that the treatment involving Methylobacterium populi TMV7–4 or Enterobacter cloacae S23 with salicylic acid, C7HSL and 3-oxo-C14 HSL significantly enhanced plant growth including root length, nodulation, pod formation, soil available nutrients and plant nutrients uptake. In future field validation is required for the use of QS molecules in improving groundnut production.http://www.sciencedirect.com/science/article/pii/S2215017X25000207N-acyl homoserine lactonePlant growth promoting traitsSalicylic acidSwimmingSwarming
spellingShingle Sivakumar Madhan
Yuvasri Errakutty Arunan
Anandham Rangasamy
Balachandar Dananjeyan
Johnson Iruthayasamy
Manimaran Gajendiran
Krishnamoorthy Ramasamy
Raghu Rajasekaran
Vincent Saminathan
Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
Biotechnology Reports
N-acyl homoserine lactone
Plant growth promoting traits
Salicylic acid
Swimming
Swarming
title Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
title_full Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
title_fullStr Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
title_full_unstemmed Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
title_short Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
title_sort rhizobial passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut
topic N-acyl homoserine lactone
Plant growth promoting traits
Salicylic acid
Swimming
Swarming
url http://www.sciencedirect.com/science/article/pii/S2215017X25000207
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