M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis
Regular monitoring of patients with a history of hepatitis C virus (HCV) infection is critical for the detection and management of hepatocellular carcinoma (HCC). Mac-2 binding protein glycosylation isomer (M2BPGi) has been used to monitor fibrosis progression and predict HCC. However, HCC predictio...
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MDPI AG
2024-11-01
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| Online Access: | https://www.mdpi.com/1420-3049/29/23/5640 |
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| author | Hiroko Shimazaki Haruki Uojima Kazumi Yamasaki Tomomi Obayashi Sayaka Fuseya Takashi Sato Masashi Mizokami Atsushi Kuno |
| author_facet | Hiroko Shimazaki Haruki Uojima Kazumi Yamasaki Tomomi Obayashi Sayaka Fuseya Takashi Sato Masashi Mizokami Atsushi Kuno |
| author_sort | Hiroko Shimazaki |
| collection | DOAJ |
| description | Regular monitoring of patients with a history of hepatitis C virus (HCV) infection is critical for the detection and management of hepatocellular carcinoma (HCC). Mac-2 binding protein glycosylation isomer (M2BPGi) has been used to monitor fibrosis progression and predict HCC. However, HCC prediction based on M2BPGi has not been optimized. Here, we identified HCC risk-related glycan signatures of M2BP using a newly developed automated bead array with multiplexed lectins. Among 955 patients with HCV who achieved sustained virological response following direct-acting antiviral treatment, we compared M2BP glycosylation from sera of 42 patients diagnosed with HCC during follow-up and 43 without HCC (control) by the lectin microarray. At the HCC observation point, we found significant differences in 17 lectins. Using an automated bead array with 12 of 17 lectins, a principal component analysis (PCA) biplot differentiated HCC from control, along the PC1 axis, explaining 75.2% of variance. Based on PC1, we generated a scoring formula for an HCC-related glycosylation signature on M2BP (M2BPgs-HCC), showing good diagnostic performance for HCC (<i>p</i> = 2.92 × 10<sup>−8</sup>, AUC = 0.829). This automated multilectin bead array improved the ability of M2BP to detect HCC, providing a candidate test for HCC surveillance in combination with other HCC markers. |
| format | Article |
| id | doaj-art-fa5d9e856d71420fac226aa4a3b2418e |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-fa5d9e856d71420fac226aa4a3b2418e2025-08-20T02:50:38ZengMDPI AGMolecules1420-30492024-11-012923564010.3390/molecules29235640M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma PrognosisHiroko Shimazaki0Haruki Uojima1Kazumi Yamasaki2Tomomi Obayashi3Sayaka Fuseya4Takashi Sato5Masashi Mizokami6Atsushi Kuno7Molecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science & Technology, Tsukuba 305-8565, JapanGenome Medical Science Project, National Center for Global Health and Medicine, Ichikawa 272-8516, JapanClinical Research Center, NHO National Hospital Organization Nagasaki Medical Center, Omura 856-0835, JapanMolecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science & Technology, Tsukuba 305-8565, JapanMolecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science & Technology, Tsukuba 305-8565, JapanMolecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science & Technology, Tsukuba 305-8565, JapanGenome Medical Science Project, National Center for Global Health and Medicine, Ichikawa 272-8516, JapanMolecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science & Technology, Tsukuba 305-8565, JapanRegular monitoring of patients with a history of hepatitis C virus (HCV) infection is critical for the detection and management of hepatocellular carcinoma (HCC). Mac-2 binding protein glycosylation isomer (M2BPGi) has been used to monitor fibrosis progression and predict HCC. However, HCC prediction based on M2BPGi has not been optimized. Here, we identified HCC risk-related glycan signatures of M2BP using a newly developed automated bead array with multiplexed lectins. Among 955 patients with HCV who achieved sustained virological response following direct-acting antiviral treatment, we compared M2BP glycosylation from sera of 42 patients diagnosed with HCC during follow-up and 43 without HCC (control) by the lectin microarray. At the HCC observation point, we found significant differences in 17 lectins. Using an automated bead array with 12 of 17 lectins, a principal component analysis (PCA) biplot differentiated HCC from control, along the PC1 axis, explaining 75.2% of variance. Based on PC1, we generated a scoring formula for an HCC-related glycosylation signature on M2BP (M2BPgs-HCC), showing good diagnostic performance for HCC (<i>p</i> = 2.92 × 10<sup>−8</sup>, AUC = 0.829). This automated multilectin bead array improved the ability of M2BP to detect HCC, providing a candidate test for HCC surveillance in combination with other HCC markers.https://www.mdpi.com/1420-3049/29/23/5640M2BPGihepatocellular carcinomaglycosylationlectin array |
| spellingShingle | Hiroko Shimazaki Haruki Uojima Kazumi Yamasaki Tomomi Obayashi Sayaka Fuseya Takashi Sato Masashi Mizokami Atsushi Kuno M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis Molecules M2BPGi hepatocellular carcinoma glycosylation lectin array |
| title | M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis |
| title_full | M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis |
| title_fullStr | M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis |
| title_full_unstemmed | M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis |
| title_short | M2BPgs-HCC: An Automated Multilectin Bead Array Indicating Aberrant Glycosylation Signatures Toward Hepatitis C Virus-Associated Hepatocellular Carcinoma Prognosis |
| title_sort | m2bpgs hcc an automated multilectin bead array indicating aberrant glycosylation signatures toward hepatitis c virus associated hepatocellular carcinoma prognosis |
| topic | M2BPGi hepatocellular carcinoma glycosylation lectin array |
| url | https://www.mdpi.com/1420-3049/29/23/5640 |
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