Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells
Protease-activated receptor 1 (PAR1) has been associated to tissue repair and bone healing. The aim of the present study was to evaluate the effect of PAR1 activation on the osteogenic activity of human periodontal ligament stem cells (PDLSCs). PDLSCs were cultured in the presence of PAR1-selective...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/6857386 |
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| author | Emanuel Silva Rovai Lucas Macedo Batitucci Ambrósio Bruno Nunes de França Letícia Rodrigues de Oliveira Letícia Miquelitto Gasparoni Carla Renata Sipert Marinella Holzhausen |
| author_facet | Emanuel Silva Rovai Lucas Macedo Batitucci Ambrósio Bruno Nunes de França Letícia Rodrigues de Oliveira Letícia Miquelitto Gasparoni Carla Renata Sipert Marinella Holzhausen |
| author_sort | Emanuel Silva Rovai |
| collection | DOAJ |
| description | Protease-activated receptor 1 (PAR1) has been associated to tissue repair and bone healing. The aim of the present study was to evaluate the effect of PAR1 activation on the osteogenic activity of human periodontal ligament stem cells (PDLSCs). PDLSCs were cultured in the presence of PAR1-selective agonist peptide (100 nM), thrombin (0.1 U/mL), or PAR1 antagonist peptide (100 nM). Calcium deposits, calcium concentration (supernatant), alkaline phosphatase activity (ALP), cell proliferation, and gene (qPCR) and protein expression (ELISA assay) of osteogenic factors were assessed at 2, 7, and 14 days. PAR1 activation led to increased calcium deposits (p<0.05), calcium concentration (p<0.05), ALP activity (p<0.05), and cell proliferation (p<0.05). Further, PAR1 activation may increase gene and protein expression of Runx2 (p<0.05) and OPG (p<0.05). In conclusion, PAR1 activation increases osteogenic activity of PDLSCs, providing a possible new strategy for periodontal regenerative therapies. |
| format | Article |
| id | doaj-art-fa50f82d04a44616989ed17f86daff6d |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-fa50f82d04a44616989ed17f86daff6d2025-08-20T02:22:37ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/68573866857386Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem CellsEmanuel Silva Rovai0Lucas Macedo Batitucci Ambrósio1Bruno Nunes de França2Letícia Rodrigues de Oliveira3Letícia Miquelitto Gasparoni4Carla Renata Sipert5Marinella Holzhausen6Division of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, BrazilDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, BrazilDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, BrazilDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, BrazilDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, BrazilDivision of Endodontics, Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, BrazilDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, BrazilProtease-activated receptor 1 (PAR1) has been associated to tissue repair and bone healing. The aim of the present study was to evaluate the effect of PAR1 activation on the osteogenic activity of human periodontal ligament stem cells (PDLSCs). PDLSCs were cultured in the presence of PAR1-selective agonist peptide (100 nM), thrombin (0.1 U/mL), or PAR1 antagonist peptide (100 nM). Calcium deposits, calcium concentration (supernatant), alkaline phosphatase activity (ALP), cell proliferation, and gene (qPCR) and protein expression (ELISA assay) of osteogenic factors were assessed at 2, 7, and 14 days. PAR1 activation led to increased calcium deposits (p<0.05), calcium concentration (p<0.05), ALP activity (p<0.05), and cell proliferation (p<0.05). Further, PAR1 activation may increase gene and protein expression of Runx2 (p<0.05) and OPG (p<0.05). In conclusion, PAR1 activation increases osteogenic activity of PDLSCs, providing a possible new strategy for periodontal regenerative therapies.http://dx.doi.org/10.1155/2019/6857386 |
| spellingShingle | Emanuel Silva Rovai Lucas Macedo Batitucci Ambrósio Bruno Nunes de França Letícia Rodrigues de Oliveira Letícia Miquelitto Gasparoni Carla Renata Sipert Marinella Holzhausen Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells Stem Cells International |
| title | Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells |
| title_full | Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells |
| title_fullStr | Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells |
| title_full_unstemmed | Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells |
| title_short | Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells |
| title_sort | protease activated receptor type 1 activation enhances osteogenic activity in human periodontal ligament stem cells |
| url | http://dx.doi.org/10.1155/2019/6857386 |
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