Determination of 19 polyphenolic compounds in tea by ultra-high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry
A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). Tea samples were extracted by 50% (V/V) ethanol, then separated by Waters Acquity BEH C18 column using...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2022-05-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001579 |
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| Summary: | A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). Tea samples were extracted by 50% (V/V) ethanol, then separated by Waters Acquity BEH C18 column using a binary solvent system composed of acetonitrile and water (0.1% formic acid) by gradient elution. The analytes were determined by Q-TOF MS in TOF MS and information dependent acquisition (IDA)-MS/MS mode. The results showed that mass accuracy error of the 19 polyphenols were lower than 5.0 × 10–6, good linear relationship was got in range of 0.2–500 μg/L and correlation coefficient was higher than 0.9990. The LOD was in the range of 0.002–0.100 mg/kg and the LOQ was in the range of 0.004–0.200 mg/kg. Recovery of the method was in range of 78.4%–109.2% with spike levels of 0.004–2.000 mg/kg, relative standard deviations were lower than 10%. The method was simple, rapid and accurate. It could be used for the rapid screening and quantitative analysis of 19 polyphenols in tea. |
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| ISSN: | 2213-4530 |