Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time
Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Advances in Urology |
| Online Access: | http://dx.doi.org/10.1155/2010/136898 |
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| _version_ | 1849694514442141696 |
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| author | Amjad Hossain Subhash Aryal Collin Osuampke John Phelps |
| author_facet | Amjad Hossain Subhash Aryal Collin Osuampke John Phelps |
| author_sort | Amjad Hossain |
| collection | DOAJ |
| description | Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA. |
| format | Article |
| id | doaj-art-fa4f2da2512b4dfc8bc1b5a954117aab |
| institution | DOAJ |
| issn | 1687-6369 1687-6377 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Urology |
| spelling | doaj-art-fa4f2da2512b4dfc8bc1b5a954117aab2025-08-20T03:20:02ZengWileyAdvances in Urology1687-63691687-63772010-01-01201010.1155/2010/136898136898Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay TimeAmjad Hossain0Subhash Aryal1Collin Osuampke2John Phelps3Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADepartment of Biostatistics, Health Science Center, University of North Texas, Fort Worth, TX 76107, USADivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USAHuman sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA.http://dx.doi.org/10.1155/2010/136898 |
| spellingShingle | Amjad Hossain Subhash Aryal Collin Osuampke John Phelps Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time Advances in Urology |
| title | Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time |
| title_full | Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time |
| title_fullStr | Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time |
| title_full_unstemmed | Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time |
| title_short | Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time |
| title_sort | human sperm bioassay for reprotoxicity testing in embryo culture media some practical considerations in reducing the assay time |
| url | http://dx.doi.org/10.1155/2010/136898 |
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