Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth

While antibiotics are occasionally used in orchard bacterial disease management, their ecological impacts remain understudied. This investigation reveals significant sublethal effects of norfloxacin on the key fruit tree pest Grapholita molesta (oriental fruit moth). Our results showed that the anti...

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Main Authors: Shaokai Liu, Xueying Liu, Donghan Wang, Junjie Li, Yanshen Fu, Chunyang Guo, Huilin Tang, Junheng Gao, Xiangqun Yuan, Yiping Li
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S014765132500658X
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author Shaokai Liu
Xueying Liu
Donghan Wang
Junjie Li
Yanshen Fu
Chunyang Guo
Huilin Tang
Junheng Gao
Xiangqun Yuan
Yiping Li
author_facet Shaokai Liu
Xueying Liu
Donghan Wang
Junjie Li
Yanshen Fu
Chunyang Guo
Huilin Tang
Junheng Gao
Xiangqun Yuan
Yiping Li
author_sort Shaokai Liu
collection DOAJ
description While antibiotics are occasionally used in orchard bacterial disease management, their ecological impacts remain understudied. This investigation reveals significant sublethal effects of norfloxacin on the key fruit tree pest Grapholita molesta (oriental fruit moth). Our results showed that the antibiotic norfloxacin induced multiple developmental alterations by reducing the abundance of Enterobacteriaceae bacteria in the larval guts and adult ovaries: larval stage prolongation (+8.64 %), reduced pupal weight (-6.95 %), shortened adult period (-9.55 %), and impaired female fecundity with 24.77 % lower egg production and 16.57 % reduced egg hatchability. Our findings demonstrate that optimized antibiotic applications could simultaneously manage bacterial pathogens and suppress pest populations, providing dual agricultural benefits. This study proposes a novel integrated approach for orchard management through targeted antibiotic utilization.
format Article
id doaj-art-fa44fc044cb541eaac48d7fa6291701a
institution Kabale University
issn 0147-6513
language English
publishDate 2025-06-01
publisher Elsevier
record_format Article
series Ecotoxicology and Environmental Safety
spelling doaj-art-fa44fc044cb541eaac48d7fa6291701a2025-08-20T03:53:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-06-0129811832210.1016/j.ecoenv.2025.118322Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female mothShaokai Liu0Xueying Liu1Donghan Wang2Junjie Li3Yanshen Fu4Chunyang Guo5Huilin Tang6Junheng Gao7Xiangqun Yuan8Yiping Li9Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCorresponding author.; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaWhile antibiotics are occasionally used in orchard bacterial disease management, their ecological impacts remain understudied. This investigation reveals significant sublethal effects of norfloxacin on the key fruit tree pest Grapholita molesta (oriental fruit moth). Our results showed that the antibiotic norfloxacin induced multiple developmental alterations by reducing the abundance of Enterobacteriaceae bacteria in the larval guts and adult ovaries: larval stage prolongation (+8.64 %), reduced pupal weight (-6.95 %), shortened adult period (-9.55 %), and impaired female fecundity with 24.77 % lower egg production and 16.57 % reduced egg hatchability. Our findings demonstrate that optimized antibiotic applications could simultaneously manage bacterial pathogens and suppress pest populations, providing dual agricultural benefits. This study proposes a novel integrated approach for orchard management through targeted antibiotic utilization.http://www.sciencedirect.com/science/article/pii/S014765132500658XGrapholita molestaAntibioticNorfloxacinGut microbiotaEnterobacteriaceaeReproduction
spellingShingle Shaokai Liu
Xueying Liu
Donghan Wang
Junjie Li
Yanshen Fu
Chunyang Guo
Huilin Tang
Junheng Gao
Xiangqun Yuan
Yiping Li
Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
Ecotoxicology and Environmental Safety
Grapholita molesta
Antibiotic
Norfloxacin
Gut microbiota
Enterobacteriaceae
Reproduction
title Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
title_full Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
title_fullStr Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
title_full_unstemmed Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
title_short Norfloxacin inhibited the growth, development and reproduction of Grapholita molesta by reducing the abundance of Enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
title_sort norfloxacin inhibited the growth development and reproduction of grapholita molesta by reducing the abundance of enterobacteriaceae bacteria in the guts of larva and ovaries of female moth
topic Grapholita molesta
Antibiotic
Norfloxacin
Gut microbiota
Enterobacteriaceae
Reproduction
url http://www.sciencedirect.com/science/article/pii/S014765132500658X
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