Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia

IntroductionCoffee is one of the most economically essential agricultural commodities worldwide and in Ethiopia in particular. Despite its importance, it is constrained by different factors. Among these, coffee leaf rust, caused by Hemileia vastatrix, and wilt diseases, caused by Gibberella xylarioi...

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Main Authors: Hailu Negesa, Gabisa Gidisa, Zenebe Wubshet, Desalegn Alemayehu, Kifle Belachew, Wakuma Merga, Lemi Beksisa, Dawit Merga, Mohammedsani Zakir
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1560091/full
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author Hailu Negesa
Gabisa Gidisa
Zenebe Wubshet
Desalegn Alemayehu
Kifle Belachew
Wakuma Merga
Lemi Beksisa
Dawit Merga
Mohammedsani Zakir
author_facet Hailu Negesa
Gabisa Gidisa
Zenebe Wubshet
Desalegn Alemayehu
Kifle Belachew
Wakuma Merga
Lemi Beksisa
Dawit Merga
Mohammedsani Zakir
author_sort Hailu Negesa
collection DOAJ
description IntroductionCoffee is one of the most economically essential agricultural commodities worldwide and in Ethiopia in particular. Despite its importance, it is constrained by different factors. Among these, coffee leaf rust, caused by Hemileia vastatrix, and wilt diseases, caused by Gibberella xylarioides, are major limiting factors of coffee production. However, Ethiopia has not yet reported a commercialized resistant variety for both of these diseases. Therefore, the present study was conducted to evaluate lowland coffee genotypes against coffee leaf rust and wilt diseases under field and greenhouse conditions, respectively.MethodsA field experiment was conducted across four locations (Agaro, Teppi, Bebeka, and Gelesha) from 2021 to 2023. A randomized complete block design with three replications was used. The experiment for wilt disease was conducted on seedlings using seedling stem-nicking inoculation techniques.ResultsThe results revealed a significant difference among the genotypes in reaction to leaf rust and wilt diseases. Among the tested genotypes, I-1, I-2, K-1, and K-2 consistently showed a highly resistant reaction to leaf rust across locations, whereas one genotype (EB-1) indicated a susceptible reaction across all locations. The highest mean leaf rust severity was recorded on EB-1 (27.1%), while the lowest severity was recorded on genotype I-2 (0.35%). Similarly, four genotypes (I-1, I-2, K-1, and K-2) showed moderate resistance to wilt disease and indicated an extended incubation period compared to the susceptible control (Geisha).DiscussionAnalysis of genotype by environment (G×E) interaction indicated a highly significant interaction (P < 0.01). Among climate factors, relative humidity and maximum temperature showed a highly significant and positive correlation with coffee leaf rust. These resistant genotypes could be used by farmers as a component of integrated disease management in coffee leaf rust-prone areas of the country. In addition, end users must integrate these genotypes with other wilt management options. Overall, these genotypes can enhance the resilience of coffee production when combined with other management strategies for coffee leaf rust and wilt diseases across the lowland coffee production areas of Ethiopia.
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spelling doaj-art-fa41ca6f88ca4565a48cb1deed9efcfe2025-08-20T02:40:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-07-011610.3389/fpls.2025.15600911560091Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern EthiopiaHailu Negesa0Gabisa Gidisa1Zenebe Wubshet2Desalegn Alemayehu3Kifle Belachew4Wakuma Merga5Lemi Beksisa6Dawit Merga7Mohammedsani Zakir8Department of Plant Pathology, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Plant Pathology, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Plant Pathology, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Coffee Breeding and Genetics, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Plant Pathology, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Coffee Breeding and Genetics, Teppi Agricultural Research Center Ethiopian Institute of Agricultural Research, Teppi, EthiopiaDepartment of Coffee Breeding and Genetics, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Coffee Breeding and Genetics, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaDepartment of Coffee Breeding and Genetics, Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Jimma, EthiopiaIntroductionCoffee is one of the most economically essential agricultural commodities worldwide and in Ethiopia in particular. Despite its importance, it is constrained by different factors. Among these, coffee leaf rust, caused by Hemileia vastatrix, and wilt diseases, caused by Gibberella xylarioides, are major limiting factors of coffee production. However, Ethiopia has not yet reported a commercialized resistant variety for both of these diseases. Therefore, the present study was conducted to evaluate lowland coffee genotypes against coffee leaf rust and wilt diseases under field and greenhouse conditions, respectively.MethodsA field experiment was conducted across four locations (Agaro, Teppi, Bebeka, and Gelesha) from 2021 to 2023. A randomized complete block design with three replications was used. The experiment for wilt disease was conducted on seedlings using seedling stem-nicking inoculation techniques.ResultsThe results revealed a significant difference among the genotypes in reaction to leaf rust and wilt diseases. Among the tested genotypes, I-1, I-2, K-1, and K-2 consistently showed a highly resistant reaction to leaf rust across locations, whereas one genotype (EB-1) indicated a susceptible reaction across all locations. The highest mean leaf rust severity was recorded on EB-1 (27.1%), while the lowest severity was recorded on genotype I-2 (0.35%). Similarly, four genotypes (I-1, I-2, K-1, and K-2) showed moderate resistance to wilt disease and indicated an extended incubation period compared to the susceptible control (Geisha).DiscussionAnalysis of genotype by environment (G×E) interaction indicated a highly significant interaction (P < 0.01). Among climate factors, relative humidity and maximum temperature showed a highly significant and positive correlation with coffee leaf rust. These resistant genotypes could be used by farmers as a component of integrated disease management in coffee leaf rust-prone areas of the country. In addition, end users must integrate these genotypes with other wilt management options. Overall, these genotypes can enhance the resilience of coffee production when combined with other management strategies for coffee leaf rust and wilt diseases across the lowland coffee production areas of Ethiopia.https://www.frontiersin.org/articles/10.3389/fpls.2025.1560091/fullcoffee genotypescoffee leaf rustcoffee wilt diseasereactionresistant
spellingShingle Hailu Negesa
Gabisa Gidisa
Zenebe Wubshet
Desalegn Alemayehu
Kifle Belachew
Wakuma Merga
Lemi Beksisa
Dawit Merga
Mohammedsani Zakir
Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia
Frontiers in Plant Science
coffee genotypes
coffee leaf rust
coffee wilt disease
reaction
resistant
title Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia
title_full Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia
title_fullStr Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia
title_full_unstemmed Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia
title_short Evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern Ethiopia
title_sort evaluating lowland coffee genotypes against leaf rust and wilt diseases in southwestern ethiopia
topic coffee genotypes
coffee leaf rust
coffee wilt disease
reaction
resistant
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1560091/full
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