Multiplex one-step RT–qPCR assays for simultaneous detection of BRV, BCoV, Escherichia coli K99+ and Cryptosporidium parvum

Multiplex one-step RT–qPCR assays for simultaneous detection of BRV, BCoV, Escherichia coli K99+ and Cryptosporidium parvum

IntroductionBovine rotavirus (BRV), bovine coronavirus (BCoV), Escherichia coli K99+ (E. coli K99+), and Cryptosporidium parvum (C. parvum) are the most common pathogens involved in calf production. These pathogens can cause calf diarrhea, leading to significant economic losses in the cattle farming...

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Bibliographic Details
Main Authors: Xinru Zhao, Min Li, Yingying Yang, Yidan Wang, Xiaoru Zheng, Dehua Yin, Haihui Gao, Huatao Li, Kaiqiang Fu, Zhi Cao
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-03-01
Series:Frontiers in Veterinary Science
Subjects:
BRV
BCoV
E. coli K99+
C. parvum
multiplex TaqMan real-time PCR
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1561533/full
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