The effect of l-ergothioneine and fetuin on blastocyst development rates
<b>Aim: </b>This study, it was aimed to determine the effect of L-ergothionine and Fetuin added to the culture medium on blastocyst development.<p> <b>Materials and Methods:</b> The material of the study consisted of oocytes collected from the ovaries taken from the aba...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2022-03-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1381 |
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| Summary: | <b>Aim: </b>This study, it was aimed to determine the effect of L-ergothionine and
Fetuin added to the culture medium on blastocyst development.<p>
<b>Materials and Methods:</b> The material of the study consisted of oocytes
collected from the ovaries taken from the abattoir. Oocytes, which were
incubated for 22 hours in maturation (IVM) medium, were kept in the incubator
for 20-22 hours with semen in fertilization (IVF) medium. After
the fertilization step, zygotes were transferred in to culture (IVC) drops
with antioxidant (Grup 1: L-ergothionine (L-Erg) 0.05 mM (n:100), Grup
2: fetuin 1 mg/ml (n:100), Grup 3: control (n:120)). The 6th and 7th-day
blastocysts development were checked. Differences in in vitro embryo production
stages were evaluated with the Chi-square test.<p>
<b>Results:</b> It was determined that 299 (93.44%) of 320 oocytes were mature.
It was determined that the maturation rates of the groups were 94%,
92%, and 94.17%, respectively. It was determined that the cleavage rates
were 92.55%, 94.57%, and 92.04%. It was observed that 65 (21.74%)
blastocysts were obtained from 299 zygotes taken into the culture stage,
and the blastocyst rates in the groups were 45.74%, 11.96%, and 18.58%,
respectively. It was determined that the statistical difference between
L-ergothionine and the other two groups in blastocyst rates was significant
(p<0.05).<p>
<b>Conclusion:</b> As a result, L-Erg was seen to improve blastocyst development
rates. By investigating the use of L-Erg in different doses, it was thought
that the dose can be used with the highest antioxidant activity in vitro
embryo production and more blastocysts could be produced. |
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| ISSN: | 1309-6958 2146-1953 |