Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress

Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress

Abstract Background Quantitative Real-time PCR (qRT-PCR) is a powerful technique to analyze gene expression patterns by measuring the relative abundance of mRNA transcription levels. The most crucial step in obtaining accurate results of qRT-PCR is to select suitable reference genes. Water temperatu...

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Bibliographic Details
Main Authors: Ping Han, Jianming Chen, Zhennan Sun, Shengjie Ren, Xubo Wang
Format: Article
Language:English
Published: BMC 2025-02-01
Series:BMC Genomics
Subjects:
Japanese flounder
Reference gene
qRT-PCR
Temperature stress
Transcriptome-wide
Online Access:https://doi.org/10.1186/s12864-025-11285-7
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