Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model
Purpose. Using the classical Ankaferd Blood Stopper (ABS) solution to create active hemostasis during partial nephrectomy (PN) may not be so effective due to insufficient contact surface between the ABS hemostatic liquid agent and the bleeding area. In order to broaden the contact surface, we genera...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2013/949460 |
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| author | Emre Huri Yavuz Beyazit Rashad Mammadov Sila Toksoz Ayse B. Tekinay Mustafa O. Guler Huseyin Ustun Murat Kekilli Mumtaz Dadali Tugrul Celik Müzeyyen Astarci Ibrahim C. Haznedaroglu |
| author_facet | Emre Huri Yavuz Beyazit Rashad Mammadov Sila Toksoz Ayse B. Tekinay Mustafa O. Guler Huseyin Ustun Murat Kekilli Mumtaz Dadali Tugrul Celik Müzeyyen Astarci Ibrahim C. Haznedaroglu |
| author_sort | Emre Huri |
| collection | DOAJ |
| description | Purpose. Using the classical Ankaferd Blood Stopper (ABS) solution to create active hemostasis during partial nephrectomy (PN) may not be so effective due to insufficient contact surface between the ABS hemostatic liquid agent and the bleeding area. In order to broaden the contact surface, we generated a chimeric hemostatic agent, ABS nanohemostat, via combining a self-assembling peptide amphiphile molecule with the traditional Ankaferd hemostat. Materials and Methods. In order to generate ABS nanohemostat, a positively charged Peptide Amphiphile (PA) molecule was synthesized by using solid phase peptide synthesis. For animal experiments, 24 Wistar rats were divided into the following 4 groups: Group 1: control; Group 2: conventional PN with only 0.5 ml Ankaferd hemostat; Group 3: conventional PN with ABS + peptide gel; Group 4: conventional PN with only 0.5 ml peptide solution. Results. Mean warm ischemia times (WITs) were 232.8 ± 56.3, 65.6±11.4, 75.5± 17.2, and 58.1±17.6 seconds in Group 1 to Group 4, respectively. Fibrosis was not different among the groups, while inflammation was detected to be significantly different in G3 and G4. Conclusions. ABS nanohemostat has comparable hemostatic efficacy to the traditional Ankaferd hemostat in the partial nephrectomy experimental model. Elucidation of the cellular and tissue effects of this chimeric compound may establish a catalytic spark and open new avenues for novel experimental and clinical studies in the battlefield of hemostasis. |
| format | Article |
| id | doaj-art-fa2d3e8afc804a84accaae7af0424b54 |
| institution | OA Journals |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-fa2d3e8afc804a84accaae7af0424b542025-08-20T02:22:41ZengWileyInternational Journal of Biomaterials1687-87871687-87952013-01-01201310.1155/2013/949460949460Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy ModelEmre Huri0Yavuz Beyazit1Rashad Mammadov2Sila Toksoz3Ayse B. Tekinay4Mustafa O. Guler5Huseyin Ustun6Murat Kekilli7Mumtaz Dadali8Tugrul Celik9Müzeyyen Astarci10Ibrahim C. Haznedaroglu11Department of Urology, Ankara Training and Research Hospital, Ulucanlar, 06340 Ankara, TurkeyDepartment of Gastroenterology, Turkiye Yuksek Ihtisas Training and Research Hospital, Sihhiye, 06100 Ankara, TurkeyInstitute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, 06800 Ankara, TurkeyInstitute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, 06800 Ankara, TurkeyInstitute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, 06800 Ankara, TurkeyInstitute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, 06800 Ankara, TurkeyDepartment of Pathology, Ankara Training and Research Hospital, Ulucanlar, 06340 Ankara, TurkeyDepartment of Gastroenterology, Turkiye Yuksek Ihtisas Training and Research Hospital, Sihhiye, 06100 Ankara, TurkeyDepartment of Urology, Ankara Training and Research Hospital, Ulucanlar, 06340 Ankara, TurkeyDepartment of Biochemistry, Fatih University Medical School, 06560 Ankara, TurkeyDepartment of Pathology, Ankara Training and Research Hospital, Ulucanlar, 06340 Ankara, TurkeyDepartment of Hematology, Hacettepe University Medical School, Sihhiye, 06100 Ankara, TurkeyPurpose. Using the classical Ankaferd Blood Stopper (ABS) solution to create active hemostasis during partial nephrectomy (PN) may not be so effective due to insufficient contact surface between the ABS hemostatic liquid agent and the bleeding area. In order to broaden the contact surface, we generated a chimeric hemostatic agent, ABS nanohemostat, via combining a self-assembling peptide amphiphile molecule with the traditional Ankaferd hemostat. Materials and Methods. In order to generate ABS nanohemostat, a positively charged Peptide Amphiphile (PA) molecule was synthesized by using solid phase peptide synthesis. For animal experiments, 24 Wistar rats were divided into the following 4 groups: Group 1: control; Group 2: conventional PN with only 0.5 ml Ankaferd hemostat; Group 3: conventional PN with ABS + peptide gel; Group 4: conventional PN with only 0.5 ml peptide solution. Results. Mean warm ischemia times (WITs) were 232.8 ± 56.3, 65.6±11.4, 75.5± 17.2, and 58.1±17.6 seconds in Group 1 to Group 4, respectively. Fibrosis was not different among the groups, while inflammation was detected to be significantly different in G3 and G4. Conclusions. ABS nanohemostat has comparable hemostatic efficacy to the traditional Ankaferd hemostat in the partial nephrectomy experimental model. Elucidation of the cellular and tissue effects of this chimeric compound may establish a catalytic spark and open new avenues for novel experimental and clinical studies in the battlefield of hemostasis.http://dx.doi.org/10.1155/2013/949460 |
| spellingShingle | Emre Huri Yavuz Beyazit Rashad Mammadov Sila Toksoz Ayse B. Tekinay Mustafa O. Guler Huseyin Ustun Murat Kekilli Mumtaz Dadali Tugrul Celik Müzeyyen Astarci Ibrahim C. Haznedaroglu Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model International Journal of Biomaterials |
| title | Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model |
| title_full | Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model |
| title_fullStr | Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model |
| title_full_unstemmed | Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model |
| title_short | Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model |
| title_sort | generation of chimeric abs nanohemostat complex and comparing its histomorphological in vivo effects to the traditional ankaferd hemostat in controlled experimental partial nephrectomy model |
| url | http://dx.doi.org/10.1155/2013/949460 |
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