Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection
Systemic infection with Cryptococcus neoformans, a dangerous and contagious pathogen found throughout the world, frequently results in lethal cryptococcal pneumonia and meningoencephalitis, and no effective treatments and vaccination of cryptococcosis are available. Here, we describe Prm1, a novel r...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2244087 |
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| author | Chao Li Yang Meng Hailong Li Wei Du Xindi Gao Chenhao Suo Yiru Gao Yue Ni Tianshu Sun Sheng Yang Tian Lan Meiling Xin Chen Ding |
| author_facet | Chao Li Yang Meng Hailong Li Wei Du Xindi Gao Chenhao Suo Yiru Gao Yue Ni Tianshu Sun Sheng Yang Tian Lan Meiling Xin Chen Ding |
| author_sort | Chao Li |
| collection | DOAJ |
| description | Systemic infection with Cryptococcus neoformans, a dangerous and contagious pathogen found throughout the world, frequently results in lethal cryptococcal pneumonia and meningoencephalitis, and no effective treatments and vaccination of cryptococcosis are available. Here, we describe Prm1, a novel regulator of C. neoformans virulence. C. neoformans prm1Δ cells exhibit extreme sensitivity to various environmental stress conditions. Furthermore, prm1Δ cells show deficiencies in the biosynthesis of chitosan and mannoprotein, which in turn result in impairment of cell wall integrity. Treatment of mice with heat-killed prm1Δ cells was found to facilitate the host immunological defence against infection with wild-type C. neoformans. Further investigation demonstrated that prm1Δ cells strongly promote pulmonary production of interferon-γ, leading to activation of macrophage M1 differentiation and inhibition of M2 polarization. Therefore, our findings suggest that C. neoformans Prm1 may be a viable target for the development of anti-cryptococcosis medications and, cells lacking Prm1 represent a promising candidate for a vaccine. |
| format | Article |
| id | doaj-art-fa073cd5159f48aca583e04047ebee63 |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-fa073cd5159f48aca583e04047ebee632025-08-20T03:31:10ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112210.1080/22221751.2023.2244087Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infectionChao Li0Yang Meng1Hailong Li2Wei Du3Xindi Gao4Chenhao Suo5Yiru Gao6Yue Ni7Tianshu Sun8Sheng Yang9Tian Lan10Meiling Xin11Chen Ding12College of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaNHC Key Laboratory of AIDS Immunology, National Clinical Research Center for Laboratory Medicine, The First Hospital of China Medical University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, People’s Republic of ChinaSystemic infection with Cryptococcus neoformans, a dangerous and contagious pathogen found throughout the world, frequently results in lethal cryptococcal pneumonia and meningoencephalitis, and no effective treatments and vaccination of cryptococcosis are available. Here, we describe Prm1, a novel regulator of C. neoformans virulence. C. neoformans prm1Δ cells exhibit extreme sensitivity to various environmental stress conditions. Furthermore, prm1Δ cells show deficiencies in the biosynthesis of chitosan and mannoprotein, which in turn result in impairment of cell wall integrity. Treatment of mice with heat-killed prm1Δ cells was found to facilitate the host immunological defence against infection with wild-type C. neoformans. Further investigation demonstrated that prm1Δ cells strongly promote pulmonary production of interferon-γ, leading to activation of macrophage M1 differentiation and inhibition of M2 polarization. Therefore, our findings suggest that C. neoformans Prm1 may be a viable target for the development of anti-cryptococcosis medications and, cells lacking Prm1 represent a promising candidate for a vaccine.https://www.tandfonline.com/doi/10.1080/22221751.2023.2244087Cryptococcus neoformansPrm1cell wallfungal vaccineIFN-γmacrophage polarization |
| spellingShingle | Chao Li Yang Meng Hailong Li Wei Du Xindi Gao Chenhao Suo Yiru Gao Yue Ni Tianshu Sun Sheng Yang Tian Lan Meiling Xin Chen Ding Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection Emerging Microbes and Infections Cryptococcus neoformans Prm1 cell wall fungal vaccine IFN-γ macrophage polarization |
| title | Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection |
| title_full | Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection |
| title_fullStr | Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection |
| title_full_unstemmed | Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection |
| title_short | Immunization with a heat-killed prm1 deletion strain protects the host from Cryptococcus neoformans infection |
| title_sort | immunization with a heat killed prm1 deletion strain protects the host from cryptococcus neoformans infection |
| topic | Cryptococcus neoformans Prm1 cell wall fungal vaccine IFN-γ macrophage polarization |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2244087 |
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