Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt

Genus Salix is among family Salicaceae, distributing in the northern hemisphere. It is represented in Egypt by two species (Salix mucronata and Salix tetrasperma). The classification of Salix at the generic and infra-generic levels is still outstanding. We have agreed to list the Egyptian species of...

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Main Authors: Walaa azmey Hassan, Rahma Alshamrani, Wafaa Amer, Emad Alsherif
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2021-03-01
Series:مجلة بغداد للعلوم
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Online Access:https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5222
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author Walaa azmey Hassan
Rahma Alshamrani
Wafaa Amer
Emad Alsherif
author_facet Walaa azmey Hassan
Rahma Alshamrani
Wafaa Amer
Emad Alsherif
author_sort Walaa azmey Hassan
collection DOAJ
description Genus Salix is among family Salicaceae, distributing in the northern hemisphere. It is represented in Egypt by two species (Salix mucronata and Salix tetrasperma). The classification of Salix at the generic and infra-generic levels is still outstanding. We have agreed to list the Egyptian species of this genus. We collected them during field trips to most Egyptian habitats; fresh and herbarium specimens were subjected to taxonomic revision based on morphological characters; scanning electron microscope (SEM) for pollen grains; isozyme analysis using esterase and peroxidase enzymes and genetic diversity using random amplified polymorphic DNA (RAPD). We recorded that both sexes of S. mucronata existed but there were only male trees from S. tetrasperma. SEM of pollen grain revealed that there were many differences between them. Also, the application of isozyme analysis giving 12 sex indicating bands. In addition, ten RAPD primers retrieved a total of 227 amplified bands (77 for female S. mucronata, 74 for male S. mucronata and 76 for male S. tetrasperma).
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series مجلة بغداد للعلوم
spelling doaj-art-fa040780e96c4a72bd5758b32f52e16d2025-08-20T03:58:04ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862021-03-0118110.21123/bsj.2021.18.1.0077Morphological, Molecular and Pollen Grain Investigations of Salix Species in EgyptWalaa azmey Hassan0Rahma Alshamrani 1Wafaa Amer Emad Alsherif 2botany department, faculty of science, beni-suef universityKing Abdulaziz UniversityBeni-Suef UniversityGenus Salix is among family Salicaceae, distributing in the northern hemisphere. It is represented in Egypt by two species (Salix mucronata and Salix tetrasperma). The classification of Salix at the generic and infra-generic levels is still outstanding. We have agreed to list the Egyptian species of this genus. We collected them during field trips to most Egyptian habitats; fresh and herbarium specimens were subjected to taxonomic revision based on morphological characters; scanning electron microscope (SEM) for pollen grains; isozyme analysis using esterase and peroxidase enzymes and genetic diversity using random amplified polymorphic DNA (RAPD). We recorded that both sexes of S. mucronata existed but there were only male trees from S. tetrasperma. SEM of pollen grain revealed that there were many differences between them. Also, the application of isozyme analysis giving 12 sex indicating bands. In addition, ten RAPD primers retrieved a total of 227 amplified bands (77 for female S. mucronata, 74 for male S. mucronata and 76 for male S. tetrasperma).https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5222Egypt; Salix; Sex segregation; Pollen grain; RAPD.
spellingShingle Walaa azmey Hassan
Rahma Alshamrani
Wafaa Amer
Emad Alsherif
Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
مجلة بغداد للعلوم
Egypt; Salix; Sex segregation; Pollen grain; RAPD.
title Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
title_full Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
title_fullStr Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
title_full_unstemmed Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
title_short Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
title_sort morphological molecular and pollen grain investigations of salix species in egypt
topic Egypt; Salix; Sex segregation; Pollen grain; RAPD.
url https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5222
work_keys_str_mv AT walaaazmeyhassan morphologicalmolecularandpollengraininvestigationsofsalixspeciesinegypt
AT rahmaalshamrani morphologicalmolecularandpollengraininvestigationsofsalixspeciesinegypt
AT wafaaamer morphologicalmolecularandpollengraininvestigationsofsalixspeciesinegypt
AT emadalsherif morphologicalmolecularandpollengraininvestigationsofsalixspeciesinegypt