Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model
We compared the functional outcome of Isl-1+ cardiac progenitors, CD90+ bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1+ cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1+/CD90+ cells were...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/3945850 |
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| author | Sergio Li Calzi Todd Cook Domenico G. Della Rocca Juan Zhang Vinayak Shenoy Yuanqing Yan Andrew Espejo Anandharajan Rathinasabapathy Max H. Jacobsen Tatiana Salazar George E. Sandusky Lynn C. Shaw Keith March Mohan K. Raizada Carl J. Pepine Michael J. Katovich Maria B. Grant |
| author_facet | Sergio Li Calzi Todd Cook Domenico G. Della Rocca Juan Zhang Vinayak Shenoy Yuanqing Yan Andrew Espejo Anandharajan Rathinasabapathy Max H. Jacobsen Tatiana Salazar George E. Sandusky Lynn C. Shaw Keith March Mohan K. Raizada Carl J. Pepine Michael J. Katovich Maria B. Grant |
| author_sort | Sergio Li Calzi |
| collection | DOAJ |
| description | We compared the functional outcome of Isl-1+ cardiac progenitors, CD90+ bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1+ cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1+/CD90+ cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1+ cells, CD90+ cells, or a combination of Isl-1+ and CD90+ cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1+cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90+ cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1+ or CD90+ cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1+ cardiac progenitors and adult bone marrow-derived CD90+ cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair. |
| format | Article |
| id | doaj-art-f9dabeab83944c5a9c5678c68a798860 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f9dabeab83944c5a9c5678c68a7988602025-02-03T05:55:18ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/39458503945850Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury ModelSergio Li Calzi0Todd Cook1Domenico G. Della Rocca2Juan Zhang3Vinayak Shenoy4Yuanqing Yan5Andrew Espejo6Anandharajan Rathinasabapathy7Max H. Jacobsen8Tatiana Salazar9George E. Sandusky10Lynn C. Shaw11Keith March12Mohan K. Raizada13Carl J. Pepine14Michael J. Katovich15Maria B. Grant16Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, AL 35294-0001, USADepartment of Medicine, IUPUI, Indianapolis, IN 46202, USADepartment of Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Pharmacodynamics, University of Florida, Gainesville, FL 32611, USADepartment of Physiology and Functional Genomics, University of Florida, Gainesville, FL 32611, USADepartment of Biostatistics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USADepartment of Pharmacodynamics, University of Florida, Gainesville, FL 32611, USAAllergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, USAPathology and Laboratory Med., IUPUI, Indianapolis, IN 46202, USADepartment of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, AL 35294-0001, USAPathology and Laboratory Med., IUPUI, Indianapolis, IN 46202, USADepartment of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, AL 35294-0001, USADepartment of Medicine, IUPUI, Indianapolis, IN 46202, USADepartment of Physiology and Functional Genomics, University of Florida, Gainesville, FL 32611, USADepartment of Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Pharmacodynamics, University of Florida, Gainesville, FL 32611, USADepartment of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, AL 35294-0001, USAWe compared the functional outcome of Isl-1+ cardiac progenitors, CD90+ bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1+ cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1+/CD90+ cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1+ cells, CD90+ cells, or a combination of Isl-1+ and CD90+ cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1+cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90+ cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1+ or CD90+ cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1+ cardiac progenitors and adult bone marrow-derived CD90+ cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair.http://dx.doi.org/10.1155/2019/3945850 |
| spellingShingle | Sergio Li Calzi Todd Cook Domenico G. Della Rocca Juan Zhang Vinayak Shenoy Yuanqing Yan Andrew Espejo Anandharajan Rathinasabapathy Max H. Jacobsen Tatiana Salazar George E. Sandusky Lynn C. Shaw Keith March Mohan K. Raizada Carl J. Pepine Michael J. Katovich Maria B. Grant Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model Stem Cells International |
| title | Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model |
| title_full | Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model |
| title_fullStr | Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model |
| title_full_unstemmed | Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model |
| title_short | Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model |
| title_sort | complementary embryonic and adult cell populations enhance myocardial repair in rat myocardial injury model |
| url | http://dx.doi.org/10.1155/2019/3945850 |
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