Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i>
We investigated the molecular mechanisms underlying azole resistance in seven <i>Candida tropicalis</i> isolates that caused candidemia and candiduria in Paraná, Brazil (2016–2022). Biofilm production, antifungal susceptibility testing, multilocus sequence typing, amplification and seque...
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MDPI AG
2025-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/11/1/24 |
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| author | Adriele Celine Siqueira Gisele Aparecida Bernardi Lavinia Nery Villa Stangler Arend Gabrielle Tomé Cordeiro Daiane Rosolen Fernanda Costa Brandão Berti Amanda Maria Martins Ferreira Thaís Muniz Vasconcelos Bianca Cruz Neves Luiza Souza Rodrigues Libera Maria Dalla-Costa |
| author_facet | Adriele Celine Siqueira Gisele Aparecida Bernardi Lavinia Nery Villa Stangler Arend Gabrielle Tomé Cordeiro Daiane Rosolen Fernanda Costa Brandão Berti Amanda Maria Martins Ferreira Thaís Muniz Vasconcelos Bianca Cruz Neves Luiza Souza Rodrigues Libera Maria Dalla-Costa |
| author_sort | Adriele Celine Siqueira |
| collection | DOAJ |
| description | We investigated the molecular mechanisms underlying azole resistance in seven <i>Candida tropicalis</i> isolates that caused candidemia and candiduria in Paraná, Brazil (2016–2022). Biofilm production, antifungal susceptibility testing, multilocus sequence typing, amplification and sequencing of <i>ERG11</i>, and quantification of <i>ERG11</i>, <i>MDR1</i>, and <i>CDR1</i> expression levels were performed. Notably, five isolates (71.4%) were from urine samples and two (28.6%) were from blood samples. All strains were biofilm producers, with levels ranging from moderate to strong. The minimum inhibitory concentration (MIC) values ranged from 8–>64 mg/L for fluconazole and 0.25–1 mg/L for voriconazole. All isolates had mutations in <i>ERG11</i>; Y132F and Y257N were predominant (71.4%), followed by Y132F and S154F (14.3%) and Y257H (14.3%). No differences in <i>ERG11</i> expression were found between the susceptible and resistant groups, but <i>MDR1</i> and <i>CDR1</i> were more highly expressed in the susceptible isolates. All the isolates contained previously unassigned diploid sequence types. The emergence of <i>C. tropicalis</i> azole resistance has been previously described in Brazil; however, the presence of resistant isolates in urine highlights the need for surveillance resistant strains in both urinary and invasive contexts. In our study mutations in <i>ERG11</i> were the main resistance mechanism identified in <i>C. tropicalis</i>. |
| format | Article |
| id | doaj-art-f9d6293cfa7943ba8a2b13b65c4fc457 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-f9d6293cfa7943ba8a2b13b65c4fc4572025-01-24T13:37:15ZengMDPI AGJournal of Fungi2309-608X2025-01-011112410.3390/jof11010024Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i>Adriele Celine Siqueira0Gisele Aparecida Bernardi1Lavinia Nery Villa Stangler Arend2Gabrielle Tomé Cordeiro3Daiane Rosolen4Fernanda Costa Brandão Berti5Amanda Maria Martins Ferreira6Thaís Muniz Vasconcelos7Bianca Cruz Neves8Luiza Souza Rodrigues9Libera Maria Dalla-Costa10Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, BrazilLaboratório Central do Estado do Paraná (LACEN-PR), São José dos Pinhais 83060-500, BrazilLaboratório Central do Estado do Paraná (LACEN-PR), São José dos Pinhais 83060-500, BrazilInstituto de Química, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, BrazilFaculdades Pequeno Príncipe (FPP), Curitiba 80230-020, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, BrazilInstituto de Química, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, BrazilWe investigated the molecular mechanisms underlying azole resistance in seven <i>Candida tropicalis</i> isolates that caused candidemia and candiduria in Paraná, Brazil (2016–2022). Biofilm production, antifungal susceptibility testing, multilocus sequence typing, amplification and sequencing of <i>ERG11</i>, and quantification of <i>ERG11</i>, <i>MDR1</i>, and <i>CDR1</i> expression levels were performed. Notably, five isolates (71.4%) were from urine samples and two (28.6%) were from blood samples. All strains were biofilm producers, with levels ranging from moderate to strong. The minimum inhibitory concentration (MIC) values ranged from 8–>64 mg/L for fluconazole and 0.25–1 mg/L for voriconazole. All isolates had mutations in <i>ERG11</i>; Y132F and Y257N were predominant (71.4%), followed by Y132F and S154F (14.3%) and Y257H (14.3%). No differences in <i>ERG11</i> expression were found between the susceptible and resistant groups, but <i>MDR1</i> and <i>CDR1</i> were more highly expressed in the susceptible isolates. All the isolates contained previously unassigned diploid sequence types. The emergence of <i>C. tropicalis</i> azole resistance has been previously described in Brazil; however, the presence of resistant isolates in urine highlights the need for surveillance resistant strains in both urinary and invasive contexts. In our study mutations in <i>ERG11</i> were the main resistance mechanism identified in <i>C. tropicalis</i>.https://www.mdpi.com/2309-608X/11/1/24<i>Candida tropicalis</i>CandidemiaCandiduriaazole resistance<i>ERG11</i> geneefflux pumps |
| spellingShingle | Adriele Celine Siqueira Gisele Aparecida Bernardi Lavinia Nery Villa Stangler Arend Gabrielle Tomé Cordeiro Daiane Rosolen Fernanda Costa Brandão Berti Amanda Maria Martins Ferreira Thaís Muniz Vasconcelos Bianca Cruz Neves Luiza Souza Rodrigues Libera Maria Dalla-Costa Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i> Journal of Fungi <i>Candida tropicalis</i> Candidemia Candiduria azole resistance <i>ERG11</i> gene efflux pumps |
| title | Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i> |
| title_full | Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i> |
| title_fullStr | Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i> |
| title_full_unstemmed | Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i> |
| title_short | Azole Resistance and <i>ERG11</i> Mutation in Clinical Isolates of <i>Candida tropicalis</i> |
| title_sort | azole resistance and i erg11 i mutation in clinical isolates of i candida tropicalis i |
| topic | <i>Candida tropicalis</i> Candidemia Candiduria azole resistance <i>ERG11</i> gene efflux pumps |
| url | https://www.mdpi.com/2309-608X/11/1/24 |
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