Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes
In the study, electrophoretic analysis of globulin storage proteins was performed in 32 samples of local and introduced soybean plants. The goal was the identification, passportization, and study of the genetic diversity of soybean genotypes. In addition, the genetic diversity index (H-) was calcula...
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| Language: | English |
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Altai State University
2024-12-01
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| Series: | Acta Biologica Sibirica |
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| Online Access: | http://journal.asu.ru/biol/article/view/16584 |
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| author | Gunay H. Zeynalova Saltanat A. Aghayeva |
| author_facet | Gunay H. Zeynalova Saltanat A. Aghayeva |
| author_sort | Gunay H. Zeynalova |
| collection | DOAJ |
| description | In the study, electrophoretic analysis of globulin storage proteins was performed in 32 samples of local and introduced soybean plants. The goal was the identification, passportization, and study of the genetic diversity of soybean genotypes. In addition, the genetic diversity index (H-) was calculated for zones (ω-, ϒ- β- and α-) according to the frequency of occurrence of patterns in the electropherograms of globulin storage proteins in the seeds of soybean plant samples. 26 spectra and 56 patterns were detected in soybean samples, and polymorphism was observed in most of them. 9 spectra and 20 patterns were observed in the ω-zone, 5 spectra and 8 patterns in the ϒ-zone, 5 spectra and 7 patterns in the β-zone, and 7 spectra and 21 patterns in the α-zone. The genetic diversity index was calculated based on Nei's formula for each of the 4 zones - ω, ϒ, β, and α. According to the calculations, genetic diversity was observed to be higher in the α - zone (H=0.927), slightly lower in the ω- (H=0.796) and ϒ- (H=0.680) zones, and the lowest in the β- zone (H= 0.480). Based on cluster analysis, genotypes were divided into 6 groups and subgroups. Based on the obtained results, electrophoretic analysis of globulin storage proteins was performed for the first time in polyacrylamide gel (A-PAGE) and polymorphism was detected among soybean genotypes. |
| format | Article |
| id | doaj-art-f9c215db80644adca0222c7670902a0d |
| institution | Kabale University |
| issn | 2412-1908 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Altai State University |
| record_format | Article |
| series | Acta Biologica Sibirica |
| spelling | doaj-art-f9c215db80644adca0222c7670902a0d2025-01-23T08:30:28ZengAltai State UniversityActa Biologica Sibirica2412-19082024-12-01101689–16971689–169710.5281/zenodo.1443866716584Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypesGunay H. Zeynalova0Saltanat A. Aghayeva1Ministry of Science and Education of the Republic of Azerbaijan, Institute of Bioresources, Nakhchivan, AzerbaijanWestern Caspian University, Baku, AzerbaijanIn the study, electrophoretic analysis of globulin storage proteins was performed in 32 samples of local and introduced soybean plants. The goal was the identification, passportization, and study of the genetic diversity of soybean genotypes. In addition, the genetic diversity index (H-) was calculated for zones (ω-, ϒ- β- and α-) according to the frequency of occurrence of patterns in the electropherograms of globulin storage proteins in the seeds of soybean plant samples. 26 spectra and 56 patterns were detected in soybean samples, and polymorphism was observed in most of them. 9 spectra and 20 patterns were observed in the ω-zone, 5 spectra and 8 patterns in the ϒ-zone, 5 spectra and 7 patterns in the β-zone, and 7 spectra and 21 patterns in the α-zone. The genetic diversity index was calculated based on Nei's formula for each of the 4 zones - ω, ϒ, β, and α. According to the calculations, genetic diversity was observed to be higher in the α - zone (H=0.927), slightly lower in the ω- (H=0.796) and ϒ- (H=0.680) zones, and the lowest in the β- zone (H= 0.480). Based on cluster analysis, genotypes were divided into 6 groups and subgroups. Based on the obtained results, electrophoretic analysis of globulin storage proteins was performed for the first time in polyacrylamide gel (A-PAGE) and polymorphism was detected among soybean genotypes.http://journal.asu.ru/biol/article/view/16584soybeangenotypeseedglobulinstorage proteingenepatternelectropherogramcluster |
| spellingShingle | Gunay H. Zeynalova Saltanat A. Aghayeva Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes Acta Biologica Sibirica soybean genotype seed globulin storage protein gene pattern electropherogram cluster |
| title | Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes |
| title_full | Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes |
| title_fullStr | Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes |
| title_full_unstemmed | Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes |
| title_short | Study of genetic diversity of globulin proteins in soybean (Glycine max (L.) Merr.) genotypes |
| title_sort | study of genetic diversity of globulin proteins in soybean glycine max l merr genotypes |
| topic | soybean genotype seed globulin storage protein gene pattern electropherogram cluster |
| url | http://journal.asu.ru/biol/article/view/16584 |
| work_keys_str_mv | AT gunayhzeynalova studyofgeneticdiversityofglobulinproteinsinsoybeanglycinemaxlmerrgenotypes AT saltanataaghayeva studyofgeneticdiversityofglobulinproteinsinsoybeanglycinemaxlmerrgenotypes |