In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis
Abstract Fusarium oxysporum f. sp albedinis (Foa) is a devastating fungus of date palms. To unravel the genetic characteristics associated with its pathogenesis, the two available genomes of Foa 133 and Foa 9 were compared with 49 genomes of 29 other pathogenic formae speciales belonging to Fusarium...
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21858-1 |
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| author | Hafida Ayada Boutayna Dhioui Hamid Mazouz Abdelhay El harrak Fatima Jaiti Bouchra Ouhmidou Mohammed Diouri Mohieddine Moumni |
| author_facet | Hafida Ayada Boutayna Dhioui Hamid Mazouz Abdelhay El harrak Fatima Jaiti Bouchra Ouhmidou Mohammed Diouri Mohieddine Moumni |
| author_sort | Hafida Ayada |
| collection | DOAJ |
| description | Abstract Fusarium oxysporum f. sp albedinis (Foa) is a devastating fungus of date palms. To unravel the genetic characteristics associated with its pathogenesis, the two available genomes of Foa 133 and Foa 9 were compared with 49 genomes of 29 other pathogenic formae speciales belonging to Fusarium oxysporum species complex (FOSC). Foa 133 and Foa 9 have genomes of 56.23 Mb and 65.56 Mb with 17460 and 19514 putative coding genes. Of these genes, 30% lack functional annotation with no similarity to characterized proteins. The remaining genes were involved in pathways essential to the fungi's life and their adaptation. Foa secretome analysis revealed that both Foa strains possess an expanded number of secreted effectors (3003 in Foa 133 and 2418 in Foa 9). Those include effectors encoded by Foa unique genes that are involved in Foa penetration (Egh16-like family), host defense mechanisms suppression (lysM family) and pathogen protection (cysteine-rich protein family). The accessory protein SIX6, which induces plant cell death, was also predicted in Foa. Further analysis of secreted CAZymes revealed an arsenal of enzymes involved in plant cell wall degradation. This arsenal includes an exclusively Foa-specific CAZyme (GH5-7). Transcription factors and membrane transporters (MFS) involved in fungicide efflux have been predicted in Foa, in addition to a variety of secondary metabolites. These comprise mycotoxins as well as chrysogin, the latter provides Foa with resistance against adverse environmental conditions. Our results revealed new Foa proteins that could be targeted in future research in order to manage Bayoud disease. |
| format | Article |
| id | doaj-art-f9af15a94910492f91cbb1dcf419065a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-f9af15a94910492f91cbb1dcf419065a2025-02-02T12:25:38ZengNature PortfolioScientific Reports2045-23222022-11-0112111810.1038/s41598-022-21858-1In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesisHafida Ayada0Boutayna Dhioui1Hamid Mazouz2Abdelhay El harrak3Fatima Jaiti4Bouchra Ouhmidou5Mohammed Diouri6Mohieddine Moumni7Biotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University of MeknèsBiotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University of MeknèsBiotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University of MeknèsBiotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University of MeknèsBiodiversity, Environment and Plant Protection Team, Faculty of Sciences and Technologies, Moulay Ismail University of MeknèsMicrobial biotechnology and bioactive molecules laboratory, Faculty of Sciences and Technologies, Sidi Mohammed Ben Abdellah University of FezBiotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University of MeknèsBiotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University of MeknèsAbstract Fusarium oxysporum f. sp albedinis (Foa) is a devastating fungus of date palms. To unravel the genetic characteristics associated with its pathogenesis, the two available genomes of Foa 133 and Foa 9 were compared with 49 genomes of 29 other pathogenic formae speciales belonging to Fusarium oxysporum species complex (FOSC). Foa 133 and Foa 9 have genomes of 56.23 Mb and 65.56 Mb with 17460 and 19514 putative coding genes. Of these genes, 30% lack functional annotation with no similarity to characterized proteins. The remaining genes were involved in pathways essential to the fungi's life and their adaptation. Foa secretome analysis revealed that both Foa strains possess an expanded number of secreted effectors (3003 in Foa 133 and 2418 in Foa 9). Those include effectors encoded by Foa unique genes that are involved in Foa penetration (Egh16-like family), host defense mechanisms suppression (lysM family) and pathogen protection (cysteine-rich protein family). The accessory protein SIX6, which induces plant cell death, was also predicted in Foa. Further analysis of secreted CAZymes revealed an arsenal of enzymes involved in plant cell wall degradation. This arsenal includes an exclusively Foa-specific CAZyme (GH5-7). Transcription factors and membrane transporters (MFS) involved in fungicide efflux have been predicted in Foa, in addition to a variety of secondary metabolites. These comprise mycotoxins as well as chrysogin, the latter provides Foa with resistance against adverse environmental conditions. Our results revealed new Foa proteins that could be targeted in future research in order to manage Bayoud disease.https://doi.org/10.1038/s41598-022-21858-1 |
| spellingShingle | Hafida Ayada Boutayna Dhioui Hamid Mazouz Abdelhay El harrak Fatima Jaiti Bouchra Ouhmidou Mohammed Diouri Mohieddine Moumni In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis Scientific Reports |
| title | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
| title_full | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
| title_fullStr | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
| title_full_unstemmed | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
| title_short | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
| title_sort | in silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with fusarium oxysporum f sp albedinis pathogenesis |
| url | https://doi.org/10.1038/s41598-022-21858-1 |
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